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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription PCR was performed with the SYBR master mix and StepOne real-time PCR machine (Applied Biosystems, Waltham, MA, USA). The thermal cycling program was as follows ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR reactions were performed on an ABI Prism 7500 Fast real-time PCR System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:5 in water and 2 µl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... 25 ng of cDNA was subjected to quantitative PCR (qPCR) on a Step One Plus™ Real-Time PCR System (Thermo Fisher) using the TaqMan Fast Universal PCR Master Mix (Thermo Fisher # 4364103) ...
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bioRxiv - Cancer Biology 2022Quote: ... Relative quantitation of gene expression was determined by real-time PCR which was carried out using SYBR® Green PCR Master Mix (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: cDNA synthesis was performed using SuperScript VILO and quantitative PCR performed using TaqMan probes and reagents with the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific) with 50ng of product per reaction ...
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bioRxiv - Physiology 2022Quote: Quantitative PCR (qPCR) (Aw et al., 2018) was performed using a ABI 7300 Real Time PCR System (Applied Biosystems, Waltham, MA, USA) and Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... as an internal control in Applied Biosystems 7500 Real Time PCR System with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... PCR was run using THUNDERBIRD SYBR qPCR Mix (TOYOBO, Osaka, Japan) on QuantStudi 12K Flex Real-Time PCR System (Thermo Fisher Scientific). The primers for transgene expression were designed using the terminator sequence of pCAMBIA1390 (Table S2) ...
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bioRxiv - Cancer Biology 2022Quote: ... This single-stranded cDNA was then used to perform quantitative real-time PCR (qRT-PCR) with specific primers using PowerUP SYBR Green 2x Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... PCRs were carried out using the standard setting in a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific, Waltham, MA, U.S.A.). The mean cycle threshold (Ct ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative reverse transcription-PCR (qRT-PCR) analyses were carried out using a PowerUp SYBR Green Master Mix (Applied Biosystems, USA) on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative-PCR (qPCR) was performed with Power SYBR Green on the 7500 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). Chromatin immunoprecipitation was performed as previously described (Garcia-Gutierrez et al ...
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bioRxiv - Neuroscience 2020Quote: ... The presence of the SARS-CoV-2 in these samples was evaluated by one-step qRT-PCR in a final volume of 25 μL per reaction in 96-well PCR plates using a thermocycler (7500t Real-time PCR system, Applied Biosystems, Applied Biosystems). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cDNA was diluted to 100 ng/μl and quantitative PCR (qPCR) was performed using TaqMan Real-Time PCR Assays for designated genes and TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... One μl of the reaction products was then used for Q-PCR in an Applied Biosystem™ 7500 Fast Real-Time PCR system (ThermoFisher Scientific) with gene-specific primers (Table S3).
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bioRxiv - Microbiology 2020Quote: DNA or cDNAs were subjected to quantitative PCR (qPCR) using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 μL of DNA or cDNA per 10 μL reaction in duplicate) ...
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bioRxiv - Immunology 2021Quote: cDNA was quantified by real-time PCR using the SYBR green method with 2x SYBR Green PCR Master Mix (Thermo Fisher Scientific) and a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using qPCRBIO SyGreen Blue Mix (PCR Biosystems) and run on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Primer sequences used were predesigned KiCqStart SYBR® Green primers (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan© assays were used to quantitate ERRα (Hs00607062_gH) ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative real-time PCR was performed using the Power SYBR Green PCR Master Mix and a Step-one Plus Real-Time PCR system (Thermo Fisher Scientific) to assess copy numbers for the mitochondrial gene cytochrome b (cytB ...
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bioRxiv - Cell Biology 2022Quote: ... DNA prepared from the whole-cell extracts or immunoprecipitated fractions was analyzed via quantitative PCR (qPCR) with the StepOnePlus Real-Time PCR system (Thermo Fisher Scientific) using Power SYBR Green PCR Master Mix© Green I Master (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: Quantification using real-time PCR was initiated using 10 ng of cDNA with SYBR Green PCR Master Mix (2×) (Applied Biosystems, 4364346) in a LightCycler II® 480 (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was performed by using the ViiA7 or QuantStudio Real-time PCR system (Thermo Fisher Scientific) using TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems, Foster City, CA, USA) in a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... 384 well-format real time PCR in a Roche Light Cycler 480 II using SYBR Green PCR reagents (#4309155, Thermo Fisher scientific). The primers used for mouse and human IFN-β mRNAs have been described before (18).
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using SYBR Green PCR mastermix (Genetix Biotech Asia Pvt. Ltd) on the QuantStudio Real-Time PCR System (Thermo Fisher Scientific). Primer sequences are provided in the supplementary table 7.
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bioRxiv - Cancer Biology 2023Quote: ... β-Actin was used as reference control and qRt-PCR was run on the PikoReal Real-Time PCR System (Thermo Fisher Scientific). The relative fold change in gene expressions were measured with the 2(−ΔΔCT ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was performed with biological triplicates and technical duplicates using QuantStudio 5 real-time PCR System and Power SYBR Green PCR Master Mix (Thermo Fisher Bioscientific) (13 ...
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bioRxiv - Cell Biology 2023Quote: ... were added to 10 µL PCR mix and fluorescence was detected using StepOnePlus™ Real-Time PCR System (Applied Biosystems™, 4376600). Data were analyzed using High Resolution Melting (HRM ...
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bioRxiv - Cell Biology 2023Quote: ... 2 pmol forward/ reverse primer pairs (sequences listed in Table S2), and the BIO SyGreen Lo-ROX mix (PCR Biosystems, London, UK) on an ImageQuantQ3 Real-time PCR System (Thermo Fisher Scientific) in sealed 96-well optical reaction plates (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... The titers of AAVs were calculated by real-time quantitative PCR using SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA). FUS-BBBO was performed on Ai9 mice to deliver AAV9s to the left hemisphere with 22 target sites ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR analysis was conducted on an Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System with Power SYBR Green PCR Master Mix (Applied Biosystems). Expression levels were determined with the delta Ct method and normalized to GAPDH mRNA ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:5 in water and 2 μl used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Thermal cycling and rRT-PCR analyses for all assays were performed on the ABI 7500 FAST real-time PCR system (Applied Biosystems, USA) with the following thermal cycling profile ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was then diluted and used as the template for real-time PCR analysis using the Power up SYBR Green PCR Master Mix (ThermoFisher Scientific, # A25779) on the QuantStudio3 Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten nanograms of cDNA were used for the real time PCR using ABI SyBr Green PCR Master mix (Applied Biosystems, Life Technologies) in QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten nanograms of cDNA were used for the real time PCR using ABI SyBr Green PCR Master mix (Applied Biosystems, Life Technologies) in QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed by using the Powerup™ SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918). TATA Binding Protein (TBP ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO) on ABI QuantStudio 6 flex Real-time PCR System (Thermo Fisher Scientific). The RT-qPCR Primer sequences for targeting respective genes are displayed in Table S1 ...
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bioRxiv - Cancer Biology 2021Quote: ... miR-122 and anti-miR-122 were purchased from Ambion and was used at 100 pmoles to transfect cells per well of a six-well plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a or miR scramble control (ThermoFisher Scientific, Waltham, MA) were mixed with PEI at an N:P ratio of 25 (the ratio of moles of PEI amine groups to nucleic acid phosphate groups ...
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bioRxiv - Microbiology 2020Quote: We performed a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher CN A47814) together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523) ...
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR reaction was prepared using the AgPath-ID-One-Step RT-PCR kit (Thermo Fisher Scientific, USA) adjusted to a volume of 12.5 μl [19] ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-PCR reaction using SYBR Green PCR Master Kit in QuantStudio 5 Systems (Applied Biosystems). Gene expression was normalized to GAPDH using the comparative CT method.
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bioRxiv - Microbiology 2020Quote: ... from the swab samples was based on Cq values obtained by reverse transcriptase quantitative real-time PCR (RT-qPCR) targeting the matrix (M) gene 63 and using AgPath-ID One-Step RT-PCR reagent kit (Thermo Fisher Scientific, Waltham, MA,USA) on a a BioRad CFX1000 Real-Time PCR System (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated viral RNA was tested by one-step RT-PCR using the Verso 1-Step RT-PCR Kit (Hot Start) (Thermo Fisher Scientific, Waltham, MA, USA) and nested primers—MarBox1F and MarBox1R ...