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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), a pre-formulated universal 2X master mix containing Dual-Lock Taq DNA polymerase for real-time PCR ...
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bioRxiv - Biochemistry 2024Quote: Thermal shift assays were performed with a StepOnePlus real-time polymerase chain reaction (PCR) machine (Life Technologies) using SYPRO Orange dye (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Transcript levels were assessed using a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). In the presence of 10-µL KAPA SYBR FAST qPCR Kit (Nippon Genetics Co ...
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bioRxiv - Cancer Biology 2024Quote: ... using an Applied Biosystems™ QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific, cat# 4471087) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was amplified using the 7300 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Darmstadt, Germany) with the following program for the SYBRGreen method ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed using the Applied Biosystems 7500 system (Applied Biosystems, Foster City, CA). Gene expression changes were calculated by the ΔCt method ...
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bioRxiv - Biochemistry 2024Quote: ... The details of the real-time-qPCR reaction using Power SYBR Green PCR Master Mix (Applied Biosystems) in a Quant Studio 12K Flex Real-Time PCR machine 285880228 (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... The amplified cDNA library was quantified using the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher) through qPCR ...
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bioRxiv - Immunology 2024Quote: ... Relative gene expression was determined using a QuantStudio 12K Flex Real-Time PCR system (Thermo Fisher Scientific) with SYBR Green and relative standard curves normalized against expression of cyclophilin a (Cypa) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The thermal cycling and data collection were performed on Quantstudio 5 Real-Time PCR instrument (Thermo Fisher), using the thermal cycling protocol 2 min at 50 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... qPCR was conducted with three biological replicates on a StepOnePlus Real-Time PCR System (Applied Biosystems, USA) using the GB-Amp™ Sybr Green qPCR Mix (GeneBio ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were created using the Superscript IV One Step RT-PCR system (Thermofisher) and primers designed based on the results of Sanger Sequencing (Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... an iterative process of RT-PCR (Superscript III platinum 1-step RT-PCR, Thermo Fisher) and topo cloning (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR reactions were performed using SuperScript III One-Step RT-PCR system (ThermoFisher, #12574030) following manufacturing protocol ...
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bioRxiv - Plant Biology 2019Quote: Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR) using the StepOne plus thermocycler (Applied Biosystems), using the Power SyBR Green master mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for DNA was performed using PowerUP SYBR Green master mix (Thermo fisher scientific, Grand Island, NY, USA) and specific primers on a Stratagene Mx3005P instrument (Agilent Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for mRNA was performed using PowerUP SYBR Green master mix (Thermo fisher scientific, Grand Island, NY, USA) and specific primers on a Stratagene Mx3005P instrument (Agilent Technology ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was performed on an Applied Biosystems StepOnePlus™ using Fast SYBR (Thermofisher). The relative change in gene expression was analyzed in triplicate using the 2-ΔΔCT method47 with β-actin as the reference gene.
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bioRxiv - Biochemistry 2019Quote: ... connected to a stainless steel emitter (Thermo Scientific, part. no. ES 542) using an EASY-nLC 1200 (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the DNA fragment encoding miRNA for Dsp (5’-TGC TGT TGA GAT GGA CAT CAA TGC ACG TTT TGG CCA CTG ACT GAC GTG CAT TGG TCC ATC TCA A-3’ and 5’-CCT GTT GAG ATG GAC CAA TGC ACG TCA GTC AGT GGC CAA AAC GTG CAT TGA TGT CCA TCT CAA C-3’) was subcloned into pcDNA6.2-GW-EmGFP-miR using BLOCK-iT Pol II miR RNAi Expression Vector Kit (Invitrogen). The EmGFP and Dsp miRNA fragments were amplified by PCR from this vector and inserted between the KpnI and EcoRV sites of the pAAV-EF1α-DIO EYFP vector (#27056 ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers nCoV_IP2-12669Fw ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Neuroscience 2024Quote: ... Two microliters of complementary DNA was diluted 5 times and added to a 20-µL reaction volume with SYBR Green real-time PCR mixture (Thermo Fisher Scientific, #A25778). Real-time PCR was performed using the QuantStudio 6 Flex real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific, A38524500) in a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific).Both batches were sequenced in a NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Zoology 2019Quote: ... followed by qPCR analysis on a 7500 Real-Time PCR System with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems, USA) using primers listed in Table 1 ...
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bioRxiv - Biophysics 2022Quote: ... was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Carlsbad CA, cat #) followed by real-time quantitative PCR (qPCR) reactions using SYBR Green (Life Technologies, Carlsbad, CA, cat #) 73 ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was performed using a real-time system and SYBR green kit (Applied Biosystems). Relative gene expression was compared to actin as an internal control ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described97 ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was measured using real-time quantitative PCR (qRT-PCR) with TaqMan™ Master Mix II no UNG (Cat 4440049, Thermofisher Scientific) with the following primer/probe sets ...
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bioRxiv - Cell Biology 2019Quote: Quantitative RT-PCRs for CAP-D2 and CAP-D3 transcripts were done in triplicates and from three independent biological samples using SYBR™ Green PCR Master Mix (Thermo Fischer Scientific) in a 7900HT Fast Real-Time PCR System (Applied Biosystems). For each reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... All reactions were performed by a 2-step PCR in triplicates followed by melting curve analysis on a ViiA7 real-time PCR cycler (Thermo Fisher Scientific). Primers are specified in Supplementary table 2 ...
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bioRxiv - Pathology 2022Quote: ... Real-time PCR reactions were performed using a QuantStudioTM 12K Flex Real-Time PCR System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (probes, Thermo Fisher Scientific). Following probes were used ...
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bioRxiv - Immunology 2022Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and QuantiStudio 5 Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: DNA or cDNAs were subjected to quantitative PCR (qPCR) using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 µL of DNA or cDNA per 10 µL reaction in duplicate) ...
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bioRxiv - Developmental Biology 2019Quote: ... Residual plasmids used for iPSC establishment were analyzed by TaqMan™ quantitative PCR using StepOnePlus™ Real-Time PCR Systems (Thermo Fisher). Primer and probe sequences (cmCAG ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... qRT-PCR and data collection were carried out on an ABI 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). The relative expression was calculated and normalized using the 2−ΔΔCt method relative to 18S and/or GUSB.
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed with a 1:2 dilution of cDNA by using the StepOnePlus Real-Time PCR-System (Thermo Fisher Scientific) or the CFX384 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was diluted 1:5 in water and used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) in a LightCycler® 96 (Roche) ...
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bioRxiv - Immunology 2021Quote: ... mRNA expression levels were analyzed using SYBR Green PCR Master Mix and a QuantStudio 12K Flex Real-Time PCR System (both from Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1 ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR (qPCR) was performed using a QuantStudio 3 Real-Time PCR System in conjunction with a SybrGreen System (Thermo Fisher Scientific) in conjunction with a SybrGreen System for the expression of Atf4 ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR reactions were performed on a StepOne Real Time PCR System using TaqMan or SYBR Green Universal PCR Master Mix (Applied Biosystems, USA) and Rn18s (18S rRNA ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using QuantStudio™ 6 Flex real-time PCR system with power SYBR® green PCR master mix (Applied Biosystems). All gene expression data were normalized by GAPDH using the comparative CT method (2-ΔΔCt) ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was prepared using 1 μg of DNase treated RNA followed by q-PCR using StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a 7900HT Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described (23) ...