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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 250 ng of total DNA was used as template for a real time PCR reaction (QuantStudio3, Thermo Fisher) using the Hot FIREPol EvaGreen qPCR kit according to the suppliers’ instructions (Solis Biodyne) ...
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bioRxiv - Pathology 2024Quote: ... cDNA was amplified with SYBR green (BIONEER 2XGreenStarqPCRMaster MIX) using a real-time PCR system (Applied Biosystems 7500) under the condition (95℃ for 3 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative real-time PCR were performed using the Power Syber Green Master Mix (Applied Biosystems, cat. no. 4368708) in a StepOne Plus RT-PCR apparatus (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... one-step cDNA synthesis and qPCR were performed on a 7500 Real-Time PCR System (Thermo Fisher Scientific) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Target gene mRNA expression was quantified by real-time PCR using iTaq Universal SYBR Green Supermix (Applied Biosystems) and normalized to ubiquitin mRNA levels ...
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bioRxiv - Microbiology 2024Quote: ... and KHQ143/KHQ144 for the rnc gene) and monitored using a StepOnePlus Real-Time PCR system (Applied Biosystems). The mRNA levels of each target gene were calculated using a standard curve of 14028s genomic DNA with known concentration and data were normalized to the levels of 16S ribosomal RNA amplified with primers 6970 and 6971.
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bioRxiv - Neuroscience 2024Quote: ... Amplification was performed on a Step One Plus Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification was performed on a Step One Plus Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the alleles amplification was performed using a QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific, Cat.#: A28567).
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bioRxiv - Cell Biology 2024Quote: ... 0.15 μM of each primer and 125 μg cDNA using a StepOneTM Real-Time PCR System (Applied Biosystems). PCR conditions were ...
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bioRxiv - Microbiology 2024Quote: ... All reactions were carried out on a 7300 ABI real-time PCR system with TaqGold polymerase (Applied Biosystems) according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2024Quote: ... and real-time quantitative PCRs were performed on an ABI PRISM 7500 using Power SYBR Green (Applied Biosystems) as previously described (Simeonova et al. ...
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bioRxiv - Neuroscience 2024Quote: ... These reactions were run in a ThermoFisher QuantStudio 5 Real-Time PCR system (Applied Biosystems, Foster City, California). All data were analyzed using the comparative Ct method (2-ΔΔCt ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was placed in the 7900HT Fast Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, USA) qPCR machine to evaluate the gene expression ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 μL reverse primer and 6.8 μL autoclaved filtered water in StepOnePlus Real-Time PCR Systems (Applied Biosystems). Gene expression was calculated as expression fold change using double-delta Ct method.
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bioRxiv - Immunology 2024Quote: ... Gene expression was quantified by QuantStudio™ 3 or 12K Flex Real-Time PCR Systems (Thermo Fisher Scientific). The mRNA expression of Gnrhr was normalized to the expression of Actb ...
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bioRxiv - Microbiology 2024Quote: ... The Real-time PCR reactions were performed using Power-Up SYBR Green Master Mix (Applied Biosystems; Lot: 2843446) in QuantStudio3 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was placed in the 7900HT Fast Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, USA) qPCR machine to evaluate the gene expression ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using powerUp SYBR Green or TaqMan Fast Advanced Master Mix (Ifnl2,3) (ThermoFisher) and using LightCycler 480 II (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... and -125b-5p (Thermo Fisher Assay ID #00449). We also measured miR-375-3p (Thermo Fisher Assay ID #00564) ...
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bioRxiv - Microbiology 2023Quote: ... RNA from blood and tissue samples from NPs were used for RT-PCR amplification using SuperScript™ IV One-Step RT-PCR System kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with a set of primers sequences targeting hantavirus nucleoprotein and polymerase genes [25,26] ...
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bioRxiv - Genomics 2020Quote: ... on a 7900HT real-time system (Applied Biosystems).
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bioRxiv - Biophysics 2019Quote: ... A real-time thermocycler QuantStudio 3 (ThermoFisher, USA) was used for collecting data from 20 to 80 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... or QuantStudio-6 Real-Time System (Applied Biosystems). Power SYBR Green Cells-to-Ct kit was used to perform quantitative PCR on 10,000 cells sorted from tumors ...
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bioRxiv - Cancer Biology 2022Quote: ... with the StepOne Real-Time System (Applied Biosystems). TaqMan gene expression assays were used ...
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bioRxiv - Molecular Biology 2022Quote: ... on ABI7500 Real-time system (Applied Biosystems, USA). Rice U6 snRNA gene was used as the control for miRNA expression analysis ...
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bioRxiv - Neuroscience 2021Quote: ... on ABI 7900HT real-time system (Applied Biosystems). GAPDH was used as a reference gene for normalization and the relative expression differences were calculated based on the 2ΛΛCt method ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using Real-Time QuantStudio7 Flex (Thermo Fisher Scientific). After 2 minutes at 50°C and 10 minutes at 94.5°C ...
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bioRxiv - Microbiology 2020Quote: Real time qPCR (SYBR®Green Applied Biosystems™ ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR analysis was performed using Power SYBR Green RNA to CT 1 step RT-PCR kit (Applied Biosystems, #4389986) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR reactions were performed with SYBR green PCR reagents using the ABI Prism 7300 sequence detection system (Applied Biosystems-Life Technologies, Waltham, MA). Relative gene expression levels were calculated using the 2 delta Ct method (Livak & Schmittgen ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using a TaqMan One-Step RT-PCR Master Mix Reagents Kit (ThermoFisher, cat. # 4309169) and primers for a genomic locus (b-actin ...
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bioRxiv - Pathology 2021Quote: ... The PCR mastermix was prepared using the AgPath-ID One-step RT-PCR kit (Thermo Fisher Scientific, USA) in a volume of 10 µl including 0.5 µl of β-Actin-mix2-HEX as internal control and 2.5 µl of extracted RNA ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed with the TaqPath™ COVID□19 CE□IVD RT□PCR Kit (Thermo Fisher Scientific) to obtain mean cycle threshold (Ct ...
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bioRxiv - Biochemistry 2023Quote: ... RT quantitative PCR was performed using the Applied Biosystems StepOnePlus™ RT PCR system (Thermo Fisher Scientific) with FastStart Universal SYBR Green Master (Roche Applied Science ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). GAPDH (AT1G13440 ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Neuroscience 2020Quote: ... using the Quant Studio OpenArray AccuFill platform and the PCR reactions were performed in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Foster City, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real time PCR was performed using a SYBR green PCR master Mix according to the manufacturer’s instructions (Applied Biosystems, Foster city, CA, USA), on an ABI prism 7900 sequence detection system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using the QuantStudio 6 Flex Real-Time PCR System and Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA). Probe sets for Il6 (Rn01410330_m1) ...
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bioRxiv - Microbiology 2022Quote: ... rRNA by qRT-PCR using TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech, Beijing, China) in a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). Each reaction included 3 μL diluted cell lysate ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of cDNA were used for quantifying various cytokine immune response-related factor cDNAs via qRT-PCR using the StepOne real-time PCR system (Applied Biosystems, Foster City, California). qRT-PCR analysis was performed using KOD SYBR qPCR Mix (Toyobo Co ...
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bioRxiv - Neuroscience 2020Quote: ... A 10 µL reaction scale TaqMan method qPCR for Gapdh was performed using 1 µL of 1:40 diluted 20 cycle PCR products with 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) or CFX384 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in 384-well reaction plates using 2X TaqMan Fast Universal Master Mix (Applied Biosystems, Foster City, CA), 20X TaqMan specific gene expression probes (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed using the PerfeCTa SYBR Green Fastmix (Quantabio, Beverly, MA) on a StepOne Real-time PCR system (Applied biosystems, Foster City, CA). PCR products were amplified with the following primer pairs ...
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bioRxiv - Genomics 2019Quote: The expression of mRNA transcripts was determined using cDNA reverse-transcribed from total RNA and quantitative PCR (qPCR) was performed on the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) using SYBR Green master mixes (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression levels of SHANK3 were determined by TaqMan® qRT-PCR reaction using QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific). The level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2022Quote: ... experiments were performed using an AB Applied Biosystems Step One Pulse Real-Time PCR System and TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA). The housekeeping gene GAPDH was used for all delta-delta Ct calculations as described earlier [57].
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR was performed in 96-well plates (PCR-96-LP-AB-C, Axygen Inc.) with SYBR® green Real-Time PCR Master mix (Thermo Fisher Scientific Inc.). The PCR program included an enzyme activation step at 95 °C (10 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... qRT-PCR was performed using the PowerUp SyBr Green Master Mix on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Scientific). Amplification was performed using the rapid cycling mode following the manufacturer’s specifications.The primers were designed based on nucleic acid sequences obtained from the National Center for Biotechnology Information and the primer sequences are displayed in Supplementary Table S1 ...