Labshake search
Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... were amplified by RT-PCR using the Verso 1-Step RT-PCR Kit with Thermo-Start Taq (Hot Start; Thermo Fisher Scientific, Waltham, MA, USA) using primers Mar- CPL-F and MarCP2R (876 bp ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by quantitative PCR on an ABI Prism 7500 real-time PCR system using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and primers amplifying a fragment of DHX37 transcript (forward 5’-CCCGATATCGAGAAAGCCTGG-3’ ...
-
bioRxiv - Microbiology 2020Quote: Quantitative PCR was performed on a QuantStudio™ 12K Flex Real-Time PCR System using a Taqman™ Universal PCR Mastermix (Applied Biosystems, Grand Island, NY). For the quantification of eubacteria and archaea ...
-
bioRxiv - Biochemistry 2020Quote: ... The quantitative RT-PCR reactions were performed on an Applied Biosystems 7500 Sequence Detection system (Applied Biosystems, Foster, CA, USA) using ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme BioTech Co.,Ltd.) ...
-
bioRxiv - Genetics 2023Quote: ... RT–PCRs of different genes using respective primers (Table S2) were performed using ABI-7300 RT–PCR with Sequence Detection System v1.4 (Applied Biosystems). Relative mRNA expression was calculated using the previously established 2−ΔΔCT method [48] ...
-
bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA was diluted 4 times with H2O and mixed with 2X TaqMan OpenArray Real Time PCR Master Mix (Life Technologies, Grand Island, NY) at a ratio of 3.8:1.2 (PCR mix:cDNA) ...
-
bioRxiv - Plant Biology 2021Quote: Initial validation of the flg22 treatment was assessed with real-time (RT) quantitative polymerase chain reactions (qPCR) on a QuantStudio 6 instrument (Thermo Fisher Scientific), using the total RNA extracted for the CAGE library preparations and the ΔΔCt method ...
-
bioRxiv - Immunology 2023Quote: DDA-SPS-MS3 method with online real-time database search (RTS) was performed on an UltiMate 3000 Dual LC nano-HPLC System (Dionex, Thermo Fisher Scientific), containing both a trapping column for peptide concentration (PepMap C18 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Reverse-transcriptase real-time quantitative polymerase chain reaction (RT-qPCR) was carried out with TaqMan® Gene Expression Assays (Thermo Fisher Scientific) in complementary deoxyribonucleic acid (cDNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... The TaqPathTM COVID-19 CE-IVD RT-PCR kit (ThermoFisher Scientific, Waltham, US) was used to identify the presence of SARS-CoV-2 ...
-
bioRxiv - Genomics 2020Quote: ... RT-PCR was performed using the Superscript III first-strand synthesis kit (Invitrogen) and cytochrome gene-specific primers (Table S1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... for RT-PCR and Arcturus PicoPure RNA isolation kit (Applied Biosystems 12204-01) for qPCR and sequencing using manual guidelines ...
-
bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using SYBR-Green PCR Master Mix kit (Applied Biosystems) with an ABI7500 real-time qPCR system (ABI Company ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were performed using the One-Step RT-PCR kit High Fidelity (Invitrogen) in a volume of 50 μl containing 5.0 μl eluted RNA with final concentrations of 1xSuperScript III One-Step RT-PCR buffer ...
-
bioRxiv - Genetics 2020Quote: ... RT-PCR products were purified using the PureLink Quick Gel Extraction Kit (Invitrogen) and identities confirmed by Sanger sequencing by Eurofins Genomics.
-
bioRxiv - Immunology 2021Quote: ... 0.1μl SuperScript III RT/Platinum Taq (CellsDirect One-Step qRT-PCR kit; Invitrogen); 0.25μl pooled 0.5μM external Vb3 primers (Vb3 ...
-
bioRxiv - Physiology 2022Quote: ... subjected to RT-PCR using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific). Resulting cDNA was then utilized for qPCR using Taqman Q-PCR probes in QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... the TaqMan RNA-to-Ct One-Step-RT-PCR kit (Applied Biosystems, # 4392938) was used along with CHIKV primers and a FAM-probe targeting the nonstructural protein 4 (CHIKV Fwd IOLqPCR-6856F ...
-
bioRxiv - Cell Biology 2020Quote: ... Brilliant SYBR Green RT-PCR (Invitrogen) was used in the qPCR mix to amplify the cDNA product ...
-
bioRxiv - Microbiology 2022Quote: ... RT-PCR grade water (Ambion #AM9935) was used for setting up all reactions ...
-
bioRxiv - Microbiology 2023Quote: ... Superscript III RT-PCR system (ThermoFisher) was used to isolate cDNA for sequence analysis ...
-
bioRxiv - Neuroscience 2023Quote: One step RT-PCR (Invitrogen #12594025) was used to isolate let-381 and unc-30 cDNAs (primers for let-381 ...
-
bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
-
bioRxiv - Microbiology 2021Quote: ... Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit; Thermo Fisher Scientific, MA, USA), Swine influenza virus [SIV] (EXOone Influenza A ...
-
bioRxiv - Microbiology 2022Quote: All RNA samples were quantified and followed by cDNA synthesis using a First-Strand cDNA Synthesis Kit for Real-Time PCR (USB-Affymetrix, Santa Clara, CA, USA; #75780). Samples were prepared according to manufacturer’s instructions for a 20-µL reaction ...
-
bioRxiv - Cell Biology 2020Quote: ... and quantified by qPCR with the quantification kit (Tian Gen company, China) for Illumina with QuantStudio 12K Flex Real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were diluted to 2 nM in each and pooled with equal volume before sequenced on Hiseq X Ten (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time PCR with three multiplex wells once with TaqMan gene assay kit (Thermo Fisher Scientific, Applied Biosystems, Carlsbad, CA, USA). The results were normalized to GAPDH ...
-
ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: Candidate SNP genotyping was performed using TaqMan® Genotyping Assay kit on ABI 7500 Real-Time PCR System from Applied Biosystems (Foster City, CA, USA). Each polymerase chain reaction sample was composed of 10 ng of DNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low) (cat# KK4873) on an Applied Biosystems ViiA 7 Real-Time qPCR / PCR Thermal Cycler System (Applied Biosystems, San Francisco, CA, USA) and was analyzed using QuantStudio software (RRID ...
-
bioRxiv - Plant Biology 2019Quote: ... qRT-PCR amplification was performed using the specific primer pairs (Table S12) on a 7500 real time PCR system (Applied Biosystems Inc., USA). The amplification process was performed according the method of Suo et al ...
-
bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described97 ...
-
bioRxiv - Cell Biology 2019Quote: ... reverse-transcribed and quantitative PCR was performed using Fast SYBR Green Master Mix in a Viia7 Real-Time PCR system (Applied Biosystems, Warrington, UK). Human primers were pre-validated Quantitect primers (Qiagen ...
-
bioRxiv - Developmental Biology 2021Quote: ... The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems, CA, USA) using SYBR Green PCR Master Mix (Takara ...
-
bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 6 Flex Real-Time PCR System using SYBR Green Power SYBR™ Green PCR-Master-Mix (Applied Biosystems™) and primers listed in Supplementary Table 2.
-
bioRxiv - Bioengineering 2021Quote: ... and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701). Data analysis was performed on https://geneglobe.qiagen.com/us/analyze ...
-
bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR to check expression of transcript levels was performed using StepOneTM Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA) with the following programme ...
-
bioRxiv - Microbiology 2020Quote: ... Real-time qualitative PCR analysis was performed as described previously (9) using an Applied Sciences StepOne Plus Real Time PCR System (Life Technologies, Carlsbad, CA). Primer and probe sequences used for gene expression analysis are listed in Supplementary Table S2 ...
-
bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was performed in a QuantStudio™ 6 Flex Real-Time PCR system instrument (384-well, Applied Biosystems, Foster City, CA) with an initial incubation at 95°C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described (23) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Standard Fast TaqMan PCR amplification was performed in triplicate on a Step One Plus Real-Time PCR System (Applied Biosystems; Grand Island, NY) in a final volume of 20 µL containing ∼10 ng of cDNA (1.5 µL of RT product) ...
-
bioRxiv - Microbiology 2022Quote: ... Real-time qualitative PCR analysis was performed as described previously(42) using an Applied Sciences Step One Plus Real Time PCR System (Life Technologies, Carlsbad, CA).Primers and probes were designed using IDT and are mentioned in supplementary material (Table:S1) ...
-
bioRxiv - Physiology 2021Quote: ... Hypothalamic gene expression levels were analyzed by quantitative PCR using a StepOnePlus Real-Time PCR System (Applied Biosystems, Inc. Foster City, CA, USA). elongation factor 1 alpha (ef1α ...
-
bioRxiv - Molecular Biology 2022Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA). PCR cycling ...
-
bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
-
bioRxiv - Neuroscience 2019Quote: ... Quantitative real-time polymerase chain reaction (qPCR) analysis was performed on an Applied Biosystems™ StepOnePlus™ Real-Time PCR Systems using Power SYBRGreen PCR Master Mix (Applied Biosystems) or TaqMan™ Gene Expression Master Mix (Applied Biosystems™) ...
-
bioRxiv - Neuroscience 2020Quote: ... The presence of the SARS-CoV-2 in these samples was evaluated by one-step qRT-PCR in a final volume of 25 μL per reaction in 96-well PCR plates using a thermocycler (7500t Real-time PCR system, Applied Biosystems, Applied Biosystems). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: Relative mRNA expression was determined by quantitative PCR (qPCR) using SYBR Green Real-Time PCR Master Mix (Applied Biosystems, Foster City, CA, USA). Total RNA was isolated from soleus muscle (10 µg ...
-
bioRxiv - Genetics 2019Quote: ... cDNA was amplified on an Applied Biosystems ViiA7 Real-Time PCR System with Power SYBR Green PCR Master Mix (ThermoFisher Scientific, Waltham, MA). GAPDH was used as control ...
-
bioRxiv - Genomics 2021Quote: qRT-PCR was performed using Quantstudio 3 Real-Time PCR system and SYBR green master mixture (Thermo Fisher Scientific, USA Catalog number: A25778). All gene expression levels were normalized to Rpl32 (in Drosophila) ...