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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The samples were then loaded onto a NuPAGE 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific Cat# NP0335BOX) and a short gel was run using NuPAGE MOPS SDS Running Buffer (Thermo Fisher Scientific Cat# NP0001 ...
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bioRxiv - Genomics 2021Quote: ... we seeded cells onto Geltrex-coated Nunc Lab-Tek 8-well chambered coverglass (Thermo Scientific, cat. 12-565-470) at a density of 4-5×104 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: ... 25 μg of protein per lane were resolved on NuPAGE Novex Bis-Tris 4-12% polyacrylamide gels (Invitrogen, #NP0321BOX) and transferred onto nitrocellulose blotting membranes (Amersham Protran ...
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bioRxiv - Cancer Biology 2021Quote: ... The dissociated epithelium was collected from the epithelial channel and digestion was quenched using Advanced DMEM/F-12 (Gibco). All single-cell samples were distributed at 5×105 cells/mL for live staining with mouse anti-human CEA IgG (Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... proteins with a molecular weight (MW) below 100 kDa were separated on 4-12% Bis/Tris gels (Life Technologies) in MES SDS Buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun to remove any debris and loaded onto a 4-12% Bis-Tris NuPAGE gel (Thermo Fisher) and separated for 3 hours at 4C at 100V ...
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bioRxiv - Neuroscience 2020Quote: ... The sample was digested with nuclease and electrophoresed on Novex TBE Gels (10%, 12 well; Thermo Fisher Scientific, EC62752BOX) and then subjected to nucleic acid staining with GelRed (Fujifilm Wako Chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... extracted nuclear proteins were subjected to NuPAGE Bis-Tris (4 to 12%) gel electrophoresis under reducing conditions (ThermoFisher Scientific) and then electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes adhered to glass coverslips in 12-well plates (1.5×106 cells/well) and were subsequently cultured for 7–9 days in RPMI (Gibco) with 10% FBS (Wisent ...
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bioRxiv - Neuroscience 2020Quote: ... and NPCs were cultured as monolayers in proliferation medium consisting of Dulbecco’s Modified Eagle Medium (DMEM)/F-12 with Glutamax (31331-093, Gibco) supplemented with 1x B-27 (17504-044 ...
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bioRxiv - Biochemistry 2020Quote: ... were then separated by 4 to 12% bis-tris SDS-PAGE in NuPAGE MOPS SDS Running Buffer (Life Technologies) either at room temperature (for Western blot ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and were transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: Full-length N was separated from the lower molecular weight proteoforms on a 0.8 mm 4-12% bis-tris SDS-PAGE gel (Invitrogen) and stained with Coomassie Blue ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the EMX1 sgRNA was confirmed by electrophoresis on a 12% TBE-UREA-PAGE gel (Thermo Scientific). Concentrations were determined by A260nm using a NanoDrop instrument (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... washed in distilled water and air dried before observation on a Philips TECNAI 12 electron microscope (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Neuroscience 2021Quote: ... to measure total protein concentrations according to manufacturer’s instructions 25 µg of total proteins of all samples were loaded onto a 4-12% gradient gel (Invitrogen) and blotted onto a PVDF membrane (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The protein samples were run on pre-cast Bis-Tris gels (NuPageTM 4–12% gradient gels, NP0322, Invitrogen™) and immunoblotting was performed ...
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bioRxiv - Neuroscience 2021Quote: ... The cell suspension was plated in poly-D-lysine culture flasks containing Dulbecco’s modified Eagle’s medium/F-12 medium (Gibco BRL) and 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples ranging from 15-40 μg were loaded onto a NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... The eluates were loaded on a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and analysed with IstantBlue staining (Merck).
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bioRxiv - Cell Biology 2021Quote: ... the insoluble pellet fractions (2 A260 units) were separated on NuPAGE 4-12% Bis-Tris 15-well gels (Invitrogen), run in NuPAGE 1x MOPS SDS running buffer (Novex) ...
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bioRxiv - Cell Biology 2021Quote: ... and insoluble pellet (0.5 A260 units) were separated on NuPAGE 4-12% Bis-Tris 26-well Midi gels (Invitrogen), run in NuPAGE 1x MES SDS running buffer (Novex).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) and supplemented with fetal bovine serum (FBS, 10%) (Gibco), Penicillin/Streptomycin (1% ...
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bioRxiv - Cell Biology 2021Quote: ... which are seeded in 12-well plates until it’s cultured at density of 85% confluence using the Lipofectamine 3000 (Invitrogen) following the protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with serum-free medium [DMEM/F-12 medium containing insulin-transferrin-selenium (ITS) (Thermo Fisher Scientific), 25 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: All proteins were separated on pre-cast 4-12 % Bis-Tris SDS-PAGE gels (NPO321BOX, NPO322BOX, NPO336BOX) from Invitrogen using MES running buffer (50mM MES ...
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bioRxiv - Systems Biology 2020Quote: ... 600 mM DTT) was added to lysates before separation on 12% Novex Tris-glycine gels (Thermo Fisher Scientific, XP00120BOX). Separated proteins were transferred onto PVDF membranes (Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Digitonin-solubilized mitochondria proteins (100 µg) were separated on pre-cast 3%-12% gradient blue native gels (Invitrogen #BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Ganglia were dissociated by trituration using a fire polished Pasteur pipette, followed by centrifugation (5 minutes, 160 x g) and trituration in wash buffer (DMEM Ham’s F-12, Gibco, 10565-018 ...
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bioRxiv - Genomics 2020Quote: SUM149 P/T cells (gift from Dr. Martin Tenniswood, SUNY Albany) were grown in Ham’s F-12 medium (Invitrogen) supplemented with 5% FBS (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... One million cells from lung cell suspension were resolved on 4 – 12 % NuPAGE Bis-Tris protein gels (Thermo Fisher), then transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (15 μg) for each sample were loaded into 4-12% gradient Bis-Tris gels (Invitrogen) and separated by electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... Protein quality and quantities were checked by SDS-PAGE electrophoresis (12% polyacrylamide gels) and UV spectrometry (Nanodrop, Thermo Scientific). A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... A549-ACE2 and A549-ACE2/TMPRSS2 cells were cultivated in DMEM/F-12 Medium with Nutrient Mix (ThermoFisher Scientific). A549-ACE2 cells further received 0.5 μg/ml puromycin ...
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bioRxiv - Microbiology 2020Quote: ... coli K-12 ΔhflX strain was complemented by reintroducing hflX ORF using pBAD replicative plasmid (Invitrogen, CAT No.: V44001) E ...
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bioRxiv - Cell Biology 2021Quote: ... Cells described above were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Biophysics 2020Quote: ... 20 mM MES pH 6.0) at 4 °C for 12 h using 3.5 kDa MWCO Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific) to remove imidazole and CHAPS ...
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bioRxiv - Cell Biology 2021Quote: ... and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX, ThermoFisher) and then transferred onto a Biotrace NT nitrocellulose membrane (27376-991 ...
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bioRxiv - Cancer Biology 2022Quote: ... which were then grown as adherent monolayers on coverslips (Φ=12 mm) coated with Geltrex (Life Technologies, A14132-01) prepared according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Immortalized human endometriotic epithelial cells (12Z cells) (16) were cultured using Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Gibco, USA) supplemented with 10% Charcoal-dextran treated fetal bovine serum (CDS-FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... TrypLE reaction was quenched via dilution with cold Splitting Media (Advanced DMEM/F-12 [Gibco], 10 mM HEPES [Invitrogen] ...
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bioRxiv - Neuroscience 2022Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using precast 4-12% polyacrylamide gradient gels (ThermoFisher, NW04122BOX), submerged at 4°C in Bolt™ MOPS SDS Running Buffer (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... Forty μg of protein extract for each sample were separated on a 4–12% NuPage Bis-Tris gel (Invitrogen) at 125 V for 100 min in NuPage MES SDS Running Buffer (Invitrogen)and transferred to nitrocellulose membranes.
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bioRxiv - Biochemistry 2022Quote: The AC16 cell line was grown in house in high glucose Dulbecco’s modified eagle medium: nutrient mixture F-12 (DMEM/F12; Gibco), with 12.5 % fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Plat-E cells at 0.3*106 cells/well of 12-well plate were seeded and cultured overnight before transfection with 100μl OptiMEM (Gibco), 3μl TransIT-293 ...
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bioRxiv - Physiology 2022Quote: ... Total RNA was reverse transcribed to cDNA with oligo(dT)12-18 and Superscript IV Reverse transcriptase (Invitrogen, 18091050). A final concentration of 0.133ng/μL was used per real time PCR reaction with InVitrogen SYBR green (ThermoFisher ...