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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were designed with non-cleavable C-terminal 6His tags and cloned between BamHI and HindIII sites into the pFastBac1 vector (Invitrogen). Point mutation variations of this initial construct were subsequently modified by Genscript from this original template ...
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bioRxiv - Immunology 2021Quote: ... with a T4 trimerization domain and a 6xHis tag at the C-terminus were codon-optimized and were subcloned into pcDNA5/FRT mammalian expression vector (ThermoFisher). Expi293 Expression system (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... were PCR-amplified and cloned into the pDEST15 vector encoding an N-terminus GST tag using the Gateway Cloning Technology (Invitrogen)27 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Systems Biology 2021Quote: ... RTKs were cloned into MAC-TAG-C expression vector (Liu et al., 2018) and pcDNA™-DEST40 (Thermo Fisher Scientific) via gateway cloning.
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bioRxiv - Microbiology 2019Quote: ... The dried peptides were reconstituted in 100 mM triethylammonium bicarbonate and labelled using 10-plex TMT (tandem mass tag) reagents according to the manufacturer’s (Thermo Scientific) protocol ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Molecular Biology 2022Quote: ... NC) or plasmid with HAX1 gene with tag on 3’end or 5’end of the gene (LipofectamineTM2000, ThermoFisher Scientific). Cells were detached and seeded on 100mm plates to grow single colonies (selection ...
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bioRxiv - Cell Biology 2022Quote: The cDNA coding for fibulin1C (Uniprot number P23142-4) with a C-terminal 6x His tag was custom-synthesized by Invitrogen and transiently transfected in HEK293 T cells using polyethylenimine in OPTIMEM (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... from each sample was labeled with 9 different TMT-10plex tags accordingly to the manufacturer’s protocols (cat# 90110, Thermo Scientific). The same mass tag and sample assignment was maintained throughout the entire study (old brain samples were labeled with TMT10-126 ...
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bioRxiv - Immunology 2022Quote: ... Gamma and Omicron NTD mutants with an N-terminal human IL-2 or Mu phosphatase signal peptide and a C-terminal polyhistidine tag followed by an AviTag were used to express soluble NTD proteins by transiently-transfecting Expi293F cells (GIBCO). After four days ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 minutes with PBS before incubation with streptavidin with fluorescent tag (Alexa Fluor 647 – Thermofisher) in blocking solution at 1:1000 dilution for 1.5hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... Human Drosha cDNA with a Flag-tag at the amino-terminus and a 6 x his tag at the carboxyl-terminus was cloned into pcDNA4/TO (Invitrogen) to construct the inducible WT Drosha expressing plasmid for immunoprecipitation assay and ubiquitination assay ...
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bioRxiv - Microbiology 2021Quote: The SARS-CoV-2 NTD (residues 13-303) or RBD (residues 319-541) with a C-terminal His tag was cloned into a modified pFastBac vector (Invitrogen) that encodes a melittin signal peptide before the NTD or RBD ...
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bioRxiv - Bioengineering 2020Quote: ... SAG1 was detected with rabbit anti-SAG1 polyclonal antibody and V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen). Primary antibodies were detected with goat polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Neuroscience 2022Quote: ... 5’ – CCT GAA ATC GCT GAT GTG TAG TCG TCA GTC AGT GGC CAA AAC GAC TAC ACA AAT CAG CGA TTT C - 3’) obtained from Invitrogen. The miRNA plasmids were then sub-cloned into an AAV2 expression backbone downstream of a CMV promoter and of an EYFP reporter sequence ...
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bioRxiv - Microbiology 2022Quote: ... which were subsequently purified and ligated upstream the His-tag into the expression vector pTrcHis2 (Invitrogen™, cat. n° V36520). The recombinant vectors were transformed into the ΔarsC E ...
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bioRxiv - Immunology 2022Quote: ... followed by an enterokinase cleavage site and a C-terminal double strep-tag was cloned into a modified pMT/BiP expression vector (pT350, Invitrogen). Drosophila S2 cells were stably co-transfected with pT350 and pCoPuro (for puromycin selection ...
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bioRxiv - Developmental Biology 2022Quote: ... using streptavidin-coated microplates and a monoclonal antibody to detect the CD4d3+4 tag (50) (Nunc Immobilizer, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... using streptavidin-coated microplates and a monoclonal antibody to detect the CD4d3+4 tag (50) (Nunc Immobilizer, Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: ... Tagged versions of BST2 WT ORF (with internal tags added after amino acid 154) plus cloning restriction enzymes sites were chemically synthesized (Invitrogen) and cloned in the pcDNA3.1 backbone ...
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bioRxiv - Microbiology 2020Quote: ... fused to a Gly-Ser-Gly linker and a Myc epitope tag into the EcoRV site of pEF1 V5 His C (Invitrogen) using NEB HiFi Assembly Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Molecular Biology 2020Quote: GAS5 RNA probes (sense/antisense) were synthesized and labeled using the FISH Tag RNA Multicolor kit (Life Technologies, Gaithersburg, MD). Skin tissue cryosections were digested with 20 µg/ml of proteinase K at 37 °C for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 µg peptides from each sample were labeled using the TMT11plex Mass Tag Label Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen), previously digested with the same enzymes ...
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibody immunolabelling was carried out for 1hr at rt with 1:2000 diluted FLAG tag mouse monoclonal antibody (FG4R, ThermoFisher). After incubation with the secondary alkaline phosphatase-conjugated goat Anti-Mouse IgG (H+L ...
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bioRxiv - Biochemistry 2019Quote: Carboxylase (with or without FLAG tag) in microsome suspensions was analyzed as above except using a Novex BIS-TRIS 4-12% gel (Invitrogen) and an antibody against the C-terminus of the carboxylase (Pudota 1998 ...
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bioRxiv - Microbiology 2019Quote: ... upstream of a Yellow Fluorescence Protein (YFP) encoding gene and an HA tag using the Gateway cloning system (ThermoFisher Scientific). The resulting plasmids were verified by sequencing and mobilized into A ...
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bioRxiv - Plant Biology 2019Quote: ... 5′-GGG GAC AGC TTT CTT GTA CAA AGT GGT TAG ATT GAA GAA GCA CCG AAT G) and cloned into pDONR221 (Invitrogen). The promoter region of ATG8e ...
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bioRxiv - Genetics 2020Quote: ... constructs for dACE2 with C-terminal Myc-DDK and GFP tags cloned in pcDNA3.4 vector were custom-synthesized by Thermo Fisher. ACE2 with C-terminal GFP-tag (RG208442 ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane and analyzed by western blotting using an anti-His tag antibody (7) or stained with Krypton fluorescent dye (ThermoFisher) according to a company’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was clarified for 10 min at 4°C at 16k rcf and incubated with 60 μl Dynabeads HIS-Tag isolation (10104D, Invitrogen) for 2 hour with rotation at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were blocked with 5% fat-free milk and thereafter probed with primary antibodies 6xHis Tag Monoclonal Antibody (Invitrogen) followed by incubation with horseradish peroxidase-labeled secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... 0.01% Tween 20 and 50μM acetyl phosphate) containing 10 μl of pre-washed (3X) magnetic cobalt beads (Dynabeads His-Tag Isolation and Pulldown - Invitrogen) for a final volume of 200 μl in 96-well plates and incubated for 40 min at room temperature on a rotating wheel ...
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bioRxiv - Biochemistry 2021Quote: ... containing the mutations C92K and K97R fused to a short GSGS linker and an AVI tag was obtained from GeneArt (Invitrogen). This construct was used to generate a stable isopeptide-linked Ube2k∼Ub conjugate ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids encoding DISP1 and DISP1-A with an HA tag at the C-terminus were transiently transfected into the cells using Lipofectamine 3000 (ThermoFisher). Two days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and then primary antibodies were added to the embryos at a dilution of 1:200 (GFP-tag (Invitrogen, MA5-15256), phospho-myosin light chain 2 (S19 ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...