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Citations for Thermo Fisher :
5151 - 5182 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Single-cell suspensions of the human iPSC-derived lung organoids were treated with 1 × Permeabilization Buffer (e-Bioscience, Thermo Fisher Scientific, Cat# 00-8333-56), and then incubated with APC-labeled anti-human HLA class 1 (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were enriched by negative selection using the Invitrogen™ Dynabeads™ Untouched™ Human NK Cells Kit (Thermo Fisher Scientific, cat# 11349D) and expanded in Gibco™ CTS™ NK-Xpander™ Medium (Gibco™ ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification of mRNA was performed by using TaqMan probes for human or murine specific α-synuclein and for selected cytokines/chemokines (Applied Biosystems Europe B.V., Zug, Switzerland). Target mRNA was normalized to tissue-specific murine GAPDH levels and displayed as relative expression after 30 amplification cycles.
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bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... CHO-K1 cells constitutively expressing human KCNE1 (designated CHO-KCNE1 cells) were generated as previously described (74) using the FLP-in™ system (Thermo Fisher Scientific, Waltham, MA, USA) and maintained under selection with hygromycin B (600 μg/mL).
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bioRxiv - Immunology 2021Quote: To investigate a link between fkbpl gene expression and psoriasis a search was performed on the NCBI GEO database to obtain mRNA microarray results and identified GSE14905 from skin biopsy samples (Affymetrix Human Genome U133 Plus 2.0 Array) which compared a non-disease control cohort and a diseased cohort ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: The Kaplan–Meier plotter was used to cross-validate the effect of differentially expressed genes in an independent ovarian cancer cohort (Geo accession identifier: GSE9891).31 This replication cohort included gene expression profiling (Affymetrix Human Genome U133 Plus 2.0 Array) of 285 ovarian tumor samples ...
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bioRxiv - Molecular Biology 2019Quote: ... falciparum or human A549 total RNA was enriched by two successive rounds of purification with the Dynabeads mRNA purification kit (Thermo Fisher # 61006, Supplementary Fig. 1b,c).
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from the six EBV-induced lymphomas (tumor group) and normal human lymphocytes (normal group) using TRIZOL reagent (Ambion/Life Sciences, Grand Island, NY, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells used for transduction were obtained by magnetic separation from healthy-donor derived apheresis products (Dynabeads® Untouched™ Human CD8+ T Cells Kit, Thermo Fisher; Magnet, DynaMag™).
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bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNAi screen targeting 10,415 druggable genes (three individual siRNAs per gene) was conducted using OV90 cells and the Silencer® Select Human Druggable Genome siRNA Library Version 4 (Ambion Thermo Fisher Scientific, Waltham, MA), in absence or presence of bardoxolone methyl ...
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bioRxiv - Immunology 2022Quote: ... the cells were first extracellularly stained with a mouse anti-human monoclonal antibody APC-Cy7 conjugated LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (#L10119, Invitrogen, Thermo Fischer Scientific, OR, USA), following a similar preparation procedure as just described for the DCs ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Bioengineering 2024Quote: Engineered cardiac tissues and hiPSC-cardiomyocytes cultured with patient IgG were fixed and incubated with secondary anti-human IgG (Thermo Fisher Scientific, cat. no. A-11013) to visualize cellular autoantibody targets ...
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were grown to ∼80% confluence followed by transfection with 100 nM human ERG Silencer Select (ID #s4811) or nontargeting siRNA oligos (Life Technologies: ERG #4392420 and nontargeting #AM4635) using lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Physiology 2023Quote: ... and human tumor necrosis factor-α (TNF-α) used in the incubation of pancreatic β cells and isolated islets were purchased from Invitrogen (Carlsbad, CA, USA). Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary anti-human CD38 antibody was applied at a dilution of 1:250 and incubated overnight at 4°C (Thermo Scientific clone RM388 Cat# MA5-36061). After washing with PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Real time PCR analysis was performed on 1µL of cDNA using TaqMan Gene Expression Master Mix according to the manufacture’s procedures using FAM labeled TaqMan® and PrimePCR gene expression human primer/probe sets (Thermo Scientific and BioRab; see Supplementary Table 3). mRNA expression was compared to untreated control using the ΔCT method ...
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bioRxiv - Immunology 2022Quote: ... and culture supernatants were harvested after 16 h and assayed for TNF-α levels using a TNF-α human uncoated ELISA kit (Invitrogen, cat. no. 85-88-7346). The assay of CHO-K1 cells (infected with lentivirus of 3A1 and CD80 ...
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bioRxiv - Cell Biology 2024Quote: Reduction of hybrid receptor expression in HUVECs was carried out by transfection of validated human siRNA duplexes of insulin receptor (Silencer™ Pre-Designed siRNA, Invitrogen; Catalog number: AM51331, siRNA ID:29) and IGF-1 receptor (Catalog number ...
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bioRxiv - Cancer Biology 2024Quote: A transfer plasmid carrying an EmGFP gene under the EF1a promoter and a blasticidin resistance gene was transfected into human embryonic kidney (HEK) 293-derived Gibco™ Viral Production Cells using the Gibco™ LV-MAX™ Transfection Kit (Thermo Fisher Scientific, cat# A35684) to produce the lentivirus (LV ...
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bioRxiv - Cell Biology 2021Quote: ... and human SK-MEL-2 (hCLTAEN-Tq2 hDNM2EN-eGFP, as previously described(Scott et al., 2018)) were maintained in DMEM (Gibco, 4.5 g/L glucose, 110 mg/L pyruvate), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Microarray transcriptomic data from UII-hFGFR3-S249C and BBN mice was combined with transcriptomic array data from human bladder tumors (CIT; Affymetrix Exon 1.0 ST; 96 MIBC and 99 NMIBC). Batch effects due to data combination were corrected using the surrogate variable analysis R package ...
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bioRxiv - Molecular Biology 2021Quote: ... The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific, Schwerte, Germany, Cat. no. R790-07) with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).