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Citations for Thermo Fisher :
5101 - 5150 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... hCMEC/D3 cells were plated in the plates or culture dishes at 6 × 104 cells/cm2 and transfected with 10 nM of negative control or human FOXO1 and ETS1 small interfering RNA (siRNA) (Tsingke Biotechnology, Beijing, China) for 12 h using Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Plasma membrane (PM) fraction was prepared from mouse or human DRG tissues and HEK cells using Mem-PER Plus Kit (Thermo Scientific, Cat No. 89842) and manufacture recommend protocol after biotinylation/streptavidin-magnetic bead (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A portion of the Fc blocked-cells were separated to accommodate the unlabeled control while remaining samples were incubated with mouse anti-human HLA-ABC W6/32 (Invitrogen [MA5-11723], 1:40) on ice for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific, Cat. #11161D) at a concentration of 25 μL/1 x 106 cells ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS at room temperature in the dark and incubated in the appropriate secondary antibody (Anti-human 488 and Anti-rabbit 647, Invitrogen, both at 1:500) at room temperature for 1 hour while on a rotary shaker (65 rpm) ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were washed with media twice and labeled using a mouse primary antibody targeting human ICAM-1 (Thermo Fisher Scientific, Cat. # 14-0549-82) at an appropriate working concentration (1:100 dilution in media ...
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bioRxiv - Cell Biology 2024Quote: ... mononucleated resident cells were isolated from human muscle as described in “Fluorescence-activated MuSC sorting and culturing” part with 7-AAD (Thermo Scientific, 00-6993-50) staining for viability selection ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Bioengineering 2024Quote: ... Bound ch5D3 was detected by goat anti-human secondary antibody conjugated to Alexa Fluor 647 (1 µg/mL; Thermo Fisher Scientific; cat.no. A-21445). All incubations were carried out 15 minutes at 4°C followed by stringent washes ...
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bioRxiv - Biochemistry 2024Quote: Media for specific human-derived stem-like spheroids (BT142, TS603, GSC923) was prepared using 500 mL DMEM: F12 media (Gibco Laboratories, Gaithersburg, MD, USA) with the following additives ...
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bioRxiv - Molecular Biology 2024Quote: ... Exons and flanking intronic regions used for pSpliceExpress minigenes were PCR amplified from human male genomic DNA using primers with attB overhangs and subsequently recombined into pSpliceExpress using BP Clonase II (Thermo Fisher, cat no. 11789020)
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Complement activation was measured by quantifying deposited C3b upon adding the wells with mouse monoclonal anti-human C3b antibody (MA1-82814, ThermoFisher Scientific, diluted 1:1,000) diluted in PBS 1X/BSA 1% and incubating 1 h at ambient temperature ...
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bioRxiv - Immunology 2024Quote: ... 2X106 Raji cells in log phase growth were incubated with 4 mg/ml biotin labeled goat anti-human IgM (Thermo Fisher Scientific, catalog #, #31778), 1 mg/ml mouse anti-human CD19 (SouthernBiotech ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific, Waltham, MA, USA).
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) containing 7 % fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: The gene expression data for the human cerebral cortex used in this study is downloaded from the NCBI Gene Expression Omnibus under the accession number GSE25219 (Affymetrix Human Exon 1.0 ST Array)(43) ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were purified using Ficoll density gradients [31] and cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing gRNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat # A32327).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Epi-Life® Growth Medium with 60 µM calcium and Cascade Biologist human keratinocyte growth supplement (HKGS) 100X (S-001–5) were from Life Technologies (Grand Island, NY, USA). The Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Immunology 2022Quote: ... operated by UNICORN 6.4, using HiTrap Protein A columns (Cytiva, catalog no. GE17-5079-01) for human IgG1 and CaptureSelect™ CH1-XL (Thermo Fisher, catalog no. 494346201) for Fab fragments ...
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bioRxiv - Cancer Biology 2019Quote: ... and the GTP-loaded Rac pulled down from the total protein lysates using a Rac1 Activation Kit (GST-human Pak1-PBD, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... HT29-MTX cells were transiently transfected with 50 µM silencing RNA (siRNA) specific for human p32 (exon 3; s2138; Thermo Fisher Scientific, Waltham, Massachusetts, US) or control siRNA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression profiling of the kidney biopsies was previously carried out using Affymetrix GeneChips (HumanGenome U133 Array and U133Plus2 Array and Affymetrix Human Gene ST GeneChip 2.1) as reported (35,36) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... parasites were maintained in vitro essentially as described previously,12 in human O- erythrocytes suspended in HEPES- and GlutamaxTM-supplemented RPMI 1640 (Thermo Fisher Scientific, order number 72400120) to which 11 mM D-glucose ...
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bioRxiv - Microbiology 2020Quote: ... S100 Calcium-binding protein A7 (S100A7) and human β-defensin 2 (DEFB4A) were amplified by using TAQMAN Gene Expression Assays (Applied Biosystems, Foster City, CA). TaqMan probes were grafted with a fluorophore (FAM ...
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bioRxiv - Cell Biology 2019Quote: ... 24 aliquots of human blood (20 μL) from each donor were pipetted into 1 mL 2D-barcoded tubes (Matrix Storage Tubes; Thermo Fisher Scientific Inc., Waltham, MA), placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12 ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Immunology 2020Quote: ... Immune mediator levels in COVID-19 patient plasma samples across acute samples were measured with by Luminex using the Cytokine/Chemokine/Growth Factor 45-plex Human ProcartaPlex™ Panel 1 (ThermoFisher Scientific, #EPX450-12171-901). Data acquisition was performed on FLEXMAP® 3D (Luminex ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Immunology 2022Quote: We isolated T cells from PBMC of patients and healthy controls for autologous mixed lymphocyte reaction (MLR) assay using Dynabeads Untouched Human CD4+ T Cells kit (Invitrogen Dynal cat. No.113.52D, US). For MLR ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNAi screen targeting 10,415 druggable genes (three individual siRNAs per gene) was conducted using OV90 cells and the Silencer® Select Human Druggable Genome siRNA Library Version 4 (Ambion Thermo Fisher Scientific, Waltham, MA), in absence or presence of bardoxolone methyl ...
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bioRxiv - Neuroscience 2023Quote: ... and growth factors was performed using a customized multiplex bead-based immunoassay - Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (#EPX650-10065-901; ThermoFisher Scientific, Waltham, MA, US) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (normal, human, adult; PCS-201-012, ATCC, VA, USA) were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2023Quote: Immunohistochemical staining for human claudin-2 was performed on formalin fixed paraffin embedded human pancreas tissue sections by using a mouse-anti claudin-2 (1:100, Invitrogen Cat# 32-5600, RRID: AB_2533085). Pancreas sections were deparaffinized ...
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
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bioRxiv - Immunology 2023Quote: ... HBEC3-KT cells were cultured in keratinocyte serum-free medium supplemented with human epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific, Grand Island, NY). MLE-15 cells were cultured in DMEM/F2 medium supplemented with 2% of fetal bovine serum and 0.5% of Insulin-Transferrin-Selenium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Single-cell suspensions of the human iPSC-derived lung organoids were treated with 1 × Permeabilization Buffer (e-Bioscience, Thermo Fisher Scientific, Cat# 00-8333-56), and then incubated with APC-labeled anti-human HLA class 1 (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...