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Citations for Thermo Fisher :
4751 - 4800 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was performed using Taqman reagents and specific primer and probe sets for human SLC22A24 (Assay ID: Hs00543210_m) and GAPDH (Assay ID: Hs99999905_m1) (Applied Biosystems, Foster City, CA). Reactions were performed in a 96-well plate with a 10 µL reaction volume using an ABI 7900HT Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem cells and human induced pluripotent stem cells (hESCs and iPSCs, Table S1) were grown using StemFlex™ Medium (A3349401, Gibco™) supplemented with Normocin™ Antimicrobial Reagent (Invivogen ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... They were then incubated with 1:200 diluted Alexa Fluor 594 conjugated goat anti-human IgG secondary antibody (Invitrogen, Thermo Fisher Scientific) for 45 min at RT and subjected to flow cytometric analysis with a CytoFLEX flow cytometer (Beckman) ...
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bioRxiv - Neuroscience 2021Quote: hiPSCs were previously generated from healthy adult human dermal fibroblast lines from a 32-year-old female from Invitrogen (C-013-5C), as described before (31) ...
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bioRxiv - Cell Biology 2019Quote: All human cells (listed in table 1) were cultured at 37°C in an atmosphere of 5% CO2 in DMEM (Thermo Fisher Scientific) supplemented with penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting NK1R-miniGs/q70 fusion construct was transfected into adherent Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using Lipofectamine 2000Ω and cells were maintained in DMEM (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... The subsequent NK1R-miniGs399fusion construct was transiently transfected into 200-mLs of Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using the Expifectamine Transfection Kit (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA deriving from LCM human pancreatic islets was then amplified using TaqMan PreAmp Master Mix (cat. 4488593, ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Vero-hSLAMF1 cells [52] stably express the human measles receptor SLAMF1 and were cultured in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific) containing 5% newborn calf serum (NCS ...
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bioRxiv - Immunology 2020Quote: ... The blocking of human mAb binding to pHA expressed on the surface of MDCK-SIAT1 cells was detected using second layer goat-anti-human IgG Alexa fluor 647 (Invitrogen A-21445) for 2-12C or streptavidin-Alexa fluor 647 (Invitrogen S21374 ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of labelled probe was combined with 5 μg salmon sperm and 10 μg human C0t1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Microbiology 2020Quote: ... HCT-8 human ileocecal colorectal adenocarcinoma cell line and its 5-FU-resistant was cultured in RPMI 1640 medium (Gibco, New Zealand) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: ... The CF cell line CFBE41ō (ΔF508/ΔF508) and normal human bronchial epithelial cells16HBEō (from D. Gruenert) were maintained in complete MEM-media (GibcoBRL, Life Technologies, MD). The normal human tracheal cell line 9HTEō ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Immunology 2021Quote: ... IgA or IgM antibodies were detected by horseradish peroxidase-conjugated goat anti-monkey/human IgG (γ-chain specific, catalog no. 62-8420, Thermo Fisher), IgM (μ-chain specific ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Immunology 2021Quote: ... 2 million cells were activated with dynabeads human T activator CD3/CD28 at a 1:1 bead to cell ratio (Thermo Fisher Scientific), Staphylococcal enterotoxin B (SEB ...
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bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells enriched as described using RosetteSep Human CD4+ enrichment cocktail were stained with antibodies against CD4 (OKT4, Thermo Fisher Scientific), CD45RA (HI100 ...
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bioRxiv - Immunology 2021Quote: Purified rested and stimulated human iNKT cells and TCONV were harvested at 48 hours for staining in either 200nM MitoTracker Green (Invitrogen cat #M7514) or 20nM tetramethylrhodamine ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293 cells (HEK 293) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, Massachusetts) supplied with 10% fetal bovine serum ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Microbiology 2020Quote: ... All parasites obtained from other sources or generated in this study were maintained under standard conditions (37) at 2% hematocrit with O+ human erythrocytes in RPMI1640 (Thermo Fisher Scientific) containing hypoxanthine ...
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bioRxiv - Molecular Biology 2022Quote: Human K562 cells (ATCC CCCL-243) were grown at 37°C and 5% CO2 in RPMI 1640 medium +GlutaMAXTM(Gibco 61870-036) supplemented with 10% FBS (HyClone SH30071 ...
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bioRxiv - Cell Biology 2022Quote: ... media was collected at the end of 48 h of lipid loading and albumin concentration was measured with a commercially available human albumin ELISA kit (Invitrogen, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... restimulated as well as an unstimulated Mel15-PBMC sample from the same timepoint using the Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen). Single ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Immunology 2022Quote: ... 11D5-3 single chain variable fragment (scFv)3 and the human heavy-chain-only FHVH3316 were codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific). Retroviral constructs encoding APRIL CARs ...
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bioRxiv - Immunology 2023Quote: The coding sequence of wild-type human CXCR4 was synthesized by General Biol (Chuzhou, China) and was cloned into the pFastBac transfer plasmid (Invitrogen, Carlsbad, CA). Heat-stabilized BRIL ...
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bioRxiv - Cancer Biology 2024Quote: Human ccRCC cell lines 786-O and RCC4 were carried in DMEM/F12 (Cytiva #SH30023) + 10% fetal bovine serum (Gibco #16000-044) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Systems Biology 2022Quote: The human monocytic cell line THP-1 (LGC, ATCC-TIB-202) was cultured and expanded in RPMI 1640 media (Gibco, 61870-010) supplemented with 10% FCS (BioConcept ...
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bioRxiv - Molecular Biology 2022Quote: ... human embryonic kidney 293T cells were seeded at a density of 13×106 cells per 15cm plate in DMEM (Thermo Fisher, 11965092) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS (cat# 16141079 ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 human colon carcinoma cells (ATCC, CCL-247) were maintained in Dulbecco’s Modified Eagle Media (DMEM) supplemented with GlutaMAX (Thermo Fisher Scientific #31966047), 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... Human neutrophil-like HL-60 cells were gifted by Orion Weiner (UCSF) and grown in supplemented RPMI medium 1640 (Gibco #22400-089) as described previously 28,54 ...
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bioRxiv - Cancer Biology 2024Quote: ... All human lung cancer cell lines were obtained from the ATCC and cultured in RPMI (Roswell Park Memorial Institute) 1640 (Gibco 11875-093) supplemented with 10%(v/v ...