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Citations for Thermo Fisher :
4801 - 4850 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Primary T-cells (CD4 and CD8; 1:1 ratio) were activated for 24 hours with Dynabeads™ Human T-Expander CD3/CD28 (Thermo Fisher) at 3:1 bead ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from these transfected cells and sent to the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet for Human Clariom™ S assay (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used (all at 1:100 dilution in immunofluorescence, 1:1000 in immunoblots): CX36 mouse anti-human (Invitrogen, clone 1E5H5), rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662) ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic®) and subcloned into pENTR (Life technologies®). GST- ...
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bioRxiv - Microbiology 2023Quote: Human pharyngeal Detroit 562 cells (ATCC CCL-138) were cultured in Gibco™ Minimum Essential Media (MEM) (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% non-heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from Illumina and the Axiom™ Genome-Wide Human Origins (HO) and the Axiom™ Precision Medicine Diversity Array Plus Kit (PMDA) from ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... of the human BPTF was amplified by PCR and subsequently cloned into the pDEST-566 vector using Gateway technology (Thermo Fisher Scientific). The resulting 6x-MBP-hBPTF (701-1073 ...
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bioRxiv - Immunology 2024Quote: ... washed in PBS/T and then incubated with 50 µL γ-chain specific goat anti-human IgG-alkaline phosphatase (AP) (Thermo Fisher) at a 1/2000 dilution for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2024Quote: ... Human A549 (mCherrypos and Luciferasepos) and HEK-293T tumor cells (in-house stocks) have been cultured in DMEM medium (Thermo Fisher 11995) supplemented like RPMI medium for T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse GBM cell lines and human prostate cancer cells were maintained in complete RPMI1640 (Media Preparation Core, Cleveland Clinic) supplemented with 10% FBS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Media Preparation Core ...
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bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... Human LRRK2 G2019S and LRRK2 Knock-out (KO) iPSC lines were generated in human iPSCs obtained from a female clone from Thermo Fisher (#A18945). Sequence information for generating targeting gRNA ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... and KYSE180 (NRF2D77V) and NRF2low KYSE450 (NRF2WT) cells (human ESCC-DSMZ, Braunschweig, Germany) 20 were cultured in Gibco RPMI1640 GlutaMAX (ThermoFisher, Waltham, MA) supplemented with 10% FBS and 1% antibiotics (penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37◦ C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... at the final concentration of 5 µM and one of the following anti-human mouse monoclonal antibodies (5 µl per sample) conjugated with allophycocyanin (APC): anti-CD9 (clone MEM-61, Thermo Fisher Scientific), anti-CD63 (clone MEM-259 ...
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bioRxiv - Physiology 2023Quote: Immortalized human microvascular endothelial cells (HMEC-1) purchased from ATCC (Manassas, VA) were cultured in antibiotic-free MCDB131 (Life Technologies, Carlsbad, CA) supplemented with 10 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: Human prostate adenocarcinoma cell line PC-3 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human prostate adenocarcinoma cell line PC-3 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: cfDNA was extracted from 600 µL of conditioned medium and 100 µL of human/murine plasma using MagMAX Cell-Free DNA Isolation Kit (ThermoFisher Scientific, A29319). The concentration of cfDNA was measured using Qubit dsDNA HS Assay Kit with Qubit 4.0 Fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Stable cell lines were transfected with an expression vector containing the full-length cDNA coding for specific human sodium channel α-subunit using Lipofectamine (Thermo Fisher). The NaV1.x stable cell lines and accessory constructs used correspond to the following GenBank accession numbers ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems). Multiwell plates of 384 wells were run on a Viia 7 PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: The region of the elephant shark genome homologous to human CNE1426 was amplified by PCR and subcloned pCR™8/GW/TOPO® (Invitrogen) (the primer sequences are in Extended Data Table 1) ...
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Cryo-electron tomography of eel sperm flagella reveals a molecular “minimum system” for motile ciliabioRxiv - Cell Biology 2023Quote: ... Danio rerio (zebrafish), Mus musculus (mouse), and human proteomes using the CPFC (Trudgian et al., 2010) or Proteomics Discoverer (Thermo Fisher Scientific) programs ...
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bioRxiv - Cancer Biology 2023Quote: Knockdown of PR130 in murine and human PDAC cells was performed by transfecting 30 pmol of validated siRNA molecules targeting the PPP2R3A/Ppp2r3a mRNAs (murine: Santa Cruz, sc-108917, 10 µM stock solution; human: Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), human/mouse Foxp3 (clone 1054c ...
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Immunology 2023Quote: ... and next intracellularly stained with PE-conjugated anti-human phospho-Akt (Ser473) (D9E, CST) and anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) manually conjugated with Alexa 488 or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed twice with PBS + 1% BSA and stained with 10 μg/mL Alexa Fluor™ 647 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific) diluted in PBS with 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Bioengineering 2023Quote: ... isolated naïve CD8+ T cells were plated at 2.5 × 105 cells/well in a 24-well plate in 2 ml cRPMI on day 0 and activated with DynaBeads Human T-Activator anti-CD3/CD28 coated microbeads (Thermo Fisher Scientific) and recombinant human IL-2 (rIL-2 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality and quantity were assessed and samples with RINS of 7 or greater were deemed suitable for further analyses with the Affymetrix Human Exon 1.0 ST Array according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA, USA). After hybridisation ...
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bioRxiv - Cell Biology 2023Quote: ... E6 medium was changed daily up to 4 days before switching to human endothelial serum free medium (hESFM) (Thermo Fisher Scientific, 11111) supplemented with 10nM bFGF (Fibroblast growth factor ...
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bioRxiv - Cell Biology 2022Quote: ... human FLOT2 (ON-TARGETplus Human FLOT2 siRNA SMART POOL, L-003666-01-0010) (Horizon Discovery Ltd.), non-targeting control siRNA (#4390843, 40 nM) (Thermo Fisher Scientific). In brief ...