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Citations for Thermo Fisher :
5051 - 5100 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA). The Sequest database search was performed with the following parameters ...
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bioRxiv - Cell Biology 2022Quote: Plasmodium falciparum 3D7 strain parasites were cultured within human O+ erythrocytes maintaining 4% haematocrit in RPMI 1640 medium (Invitrogen Corp., San Diego, CA, USA) supplemented with Albumax I (0.5% ...
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bioRxiv - Biochemistry 2020Quote: ... The human Bip 19-654aa (referred to as Bip)-encoding DNA was cloned into a modified pProEx™-HTb vector (Thermo Fisher Scientific Inc.), resulting in a construct with a N-terminal 6xHis tag ...
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bioRxiv - Microbiology 2021Quote: ... A donkey anti-mouse IgG-Alexa Fluor 555 antibody (SG2-2E6) or goat anti-human IgG-Alexa Fluor 488 antibody (93k) (Life Technologies, Grand Island, NY) was used to detect bound anti-VZV gB mAb ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... The technical replicates of the retrieved RNA were pooled prior rRNA depletion, which was performed using the human riboPOOL™ (siTOOLsBiotech, Germany) and Streptavidin-coated magnetic beads (Thermo Fisher Scientific, Norway) following the two-step protocol of the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
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bioRxiv - Immunology 2021Quote: ... Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA).
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bioRxiv - Immunology 2022Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells (human cervical ATCC CCL-2) and RK13 (Rabbit kidney cells, ATCC CCL37) were grown in Minimum Essential Medium (MEM; Gibco, Thermo Fischer, Lutterworth, UK) supplemented as described above for BSC-1 cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Genomics 2021Quote: The pulmonary HLA-DR and CD3-positive cells were isolated using superparamagnetic beads coupled with anti-human CD3 and Pan Mouse IgG antibodies (Invitrogen Dynabeads®, Life Technologies AS, Norway) and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Cancer Biology 2021Quote: ... and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, California, USA) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... selected human splenic T cells from BLTS-humanized mice and human T cells from PBMCs were seeded at 8×10^4 cells per well (96-well plate) and stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and PBS was used as vehicle control ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: Supernatants from 48-hour rested and stimulated iNKT cells and TCONV were assayed for cytokine levels of IFN-γ using human ELISA kit (Invitrogen cat #88-7316) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a 50-fold dilution of PE-conjugated goat anti-human IgM or IgG (both from Thermo Fisher, Waltham, MA, US) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The human embryonic kidney 293T/17 cells (ATCC, CRL-11268) were maintained in maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Genomics 2021Quote: ... FACS sorted BST2+ T cells and BST2− T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, cat#11161D, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: MACS enriched CD4+ T cells and CD3+ T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, Cat# 11131D, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... hCMEC/D3 cells were plated in the plates or culture dishes at 6 × 104 cells/cm2 and transfected with 10 nM of negative control or human FOXO1 and ETS1 small interfering RNA (siRNA) (Tsingke Biotechnology, Beijing, China) for 12 h using Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Immunology 2024Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of supernatant was analyzed using Cytokine 35-Plex Human Panel for the Luminex™ platform (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) on the Luminex MAGPIX ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2023Quote: ... Residual human IgG in the DDS and in the ocular tissues was determined using goat anti-human IgG antibody (A-21089, Thermo Scientific, Waltham, MA, USA). The total number and area of cells expressing CD45 in corneal sections were determined using the “Analyze Particles” tool in ImageJ software ...
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bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
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bioRxiv - Microbiology 2022Quote: The plasmids pVRC containing human codon-optimized RBD constructs were transiently transfected into Expi293F cells using the ExpiFectamine™ 293 Transfection Kit (Thermo Fisher, Waltham, MA). Briefly ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 T cells per well were stimulated for 24h with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher Scientific, 11161D) according to manufacturer’s instructions in a 24-well plate containing X-VIVO 15 media (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... NKG2D neutralization was performed by first pre-incubating 200k NK cells with 100ug/mL anti-Human CD314 (NKG2D) antibody (Invitrogen cat. #14-5878-82). Cells were spun down at 300xg for 10 minutes after which the antibody concentration was diluted to 50ug/mL for the duration of the 4-day co-culture with anti-CD3/CD28/CD2 activation beads and 100k CD8 T cells in the presence of IL2 (2ng/mL ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of plasma cytokines and chemokines were measured via Luminex xMAP technology utilizing the Procartaplex Human Cytokine/Chemokine Convenience Panel 1A 34-plex (Thermo Fisher EPXR340-12167-901) in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: SEES2 ESCs24 were cultured on irradiated MEFs (irrMEFs) with medium for human ESCs: Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% Knockout-Serum Replacement (KO-SR ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with TBS-BSA and then incubated with TBS-BSA and 20 μg/mL Alexa 594-conjugated human transferrin (Thermo Fisher Scientific; cat# T13343) for 1h at RT ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Pathology 2023Quote: Human VICs (P10462, Innoprot, Bizkaia, Spain) were maintained in Fibroblast Medium (P60108, Innoprot) supplemented with 10% FBS (10270-106, Gibco, Grand Island, NY, USA), sodium pyruvate ...