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Citations for Thermo Fisher :
5001 - 5050 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Immunology 2023Quote: ... the mice received an intravitreal injection of either human monocytes or hMdɸs that were labeled with the Vybrant DiO tracer (Invitrogen-Molecular Probes, Carlsbad, CA), and then returned to the standard light/dark cycle ...
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bioRxiv - Cell Biology 2023Quote: SEES2 ESCs24 were cultured on irradiated MEFs (irrMEFs) with medium for human ESCs: Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% Knockout-Serum Replacement (KO-SR ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed once by Hanks’ Balanced Salt Solution (HBSS) and then incubated with 2 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) or DyLight 594-conjugated goat anti-human IgG (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: Primary human CD4+ T cells expressing CBh-ZIM3-dCas9-Bsd (transduced and selected as described above) were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D) at 1:1 cell:bead ratio and ...
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bioRxiv - Biophysics 2023Quote: The nucleotide sequence encoding the human ADORA2A (1-316 aa) (UniProt ID C9JQD8) was codon optimized for Leishmania tarentolae expression by the GeneOptimizer software (Thermo Fisher Scientific, USA) and synthesized de novo (Eurofins ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells stably expressing the human hERG channel (Bioprojet, France) were cultured in the same medium with 400 µg/mL G418 (Thermo Fisher Scientific, France), at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Cell Biology 2023Quote: ... The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30) cells was cultured in Advanced MEM culture medium (Gibco™ 12492-013) supplemented with 2.5% FCS and 4 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... Descemet’s membrane with adherent endothelial cells were stripped from the corneoscleral buttons and incubated overnight at 37°C in minimal medium (Human Endothelial SFM, Gibco, cat# 11111-044); 2% charcoal stripped fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat#CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: Whole blood was obtained from healthy human donors by venipuncture and collected in BD Vacutainer™ plastic blood collection tubes with K2 EDTA: Hemogard™ Closure (Thermo Fisher) to prevent coagulation following an approved protocol from the IRB at the University of Colorado Boulder (22-0175) ...
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: A total of 3×105 CD45Δ T cells (from 2 healthy donors) and T cells (from 2 healthy donors) (effector cells) were mixed with Dynabeads Human T Activator CD3/CD28 (Thermo Fisher Scientific, USA) on the 8th-16th days after knockout of CD45 (E:beads ratio was 1:1 ...
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bioRxiv - Physiology 2023Quote: Confluent HUVECs were stimulated by 50 ng/ml human VEGF-A for indicated lengths of time and lysed in M-PER buffer (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Microbiology 2023Quote: Human pluripotent stem cells (hPSCs) lines, IMR90 (IMR90-4, WiCell), H1 (WAe001-A, WiCell) and Gibco (Gibco® Episomal hiPSC Line, Gibco®) were routinely cultured on treated 6-well tissue culture plates (CoStar® ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no. Hs00427620_m1). Custom primers and probes were ordered from Genscript to quantify the different splice variants (see Table S1 for sequences and Figure 2 for alignment on the minigene sequence) ...
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bioRxiv - Cell Biology 2023Quote: ... Ovine (Fig. 5) and human Pcells (supplement Fig.5S) were treated with monoclonal mouse SERCA2 antibody (clone 2A7-A1; Thermo Fisher; see supplement) and secondary fluorescent antibody Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: Immortalized human retinal pigment epithelial cells (RPE-1hTERT, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) containing 4.5 g/L glucose and supplemented with 10% fetal bovine serum (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) diluted in HBSS/1% BSA for 1 hour at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide arrays were washed 3 times for 5 min at RT with TBST and incubated with the antibody Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher, 1/1000) diluted in TBST ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-human IgG conjugated with horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:2000 (Life technologies, Ref H10307) in PBS-T-milk for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... The human mAb 860-55D against intact capsids was purchased from Mikrogen (Neuried, Germany) and detected using the pAb Alexa Fluor® 488 conjugated goat anti-human (A11013, Thermo Fisher Scientific). The mouse mAb 3113-81C against viral capsid proteins was obtained from US Biological (Boston ...
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bioRxiv - Molecular Biology 2024Quote: Human osteosarcoma cell line U2OS (ATCC-HTB-96) and human colorectal carcinoma cell line HCT116 (ATCC-CCL-247) were cultured in DMEM (Gibco, 11-965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Cell Biology 2024Quote: ... The hTERT RPE-1 human epithelial cell line (ATCC-CRL4000) was obtained from ATCC and grown in DMEM/F12 (ThermoFisher Scientific, Carlsbad, CA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% CO2, and 95% humidity. GFP labeled Human Brain Vascular Pericytes (HBVP) (ScienceCell™, Cat. #: 1200) were grown in DMEM (Gibco, Cat. # 11995065) supplemented with 10% FBS and 1X Antibiotic-Antimycotic under the cell culture conditions indicated above ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic kidney (HEK) 293T cells were from ATCC (cat. #CRL-3216) and were cultured in high-glucose DMEM (Gibco, cat. #41966-052) supplemented with 10% FBS and 1% Penicillin-Streptomycin.
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... the causal relation that a target gene is up- or downregulated depending on the transcription complex being active or inactive and (ii) the causal relation that a signal from a gene expression microarray (e.g. Affymetrix Human Genome U133 Plus 2.0) probeset is high or low depending on the target gene being up or down ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... In a second set of experiments Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) medium (Life Technologies Green Island NY). When the cells reached 70% confluence ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted from EndoC-βH1 and collagenase-isolated human pancreatic islets samples was quantified using Qubit 3000 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA), while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... For cells freshly purifed from human lung tissues were directly lysed using 100 μl lysis buffer from PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204). First Strand cDNA synthesis was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... according to manufacturer’s protocol Following ChIP-valuated antibodies were used for precipitation: mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA) and mouse-anti-IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Molecular Biology 2020Quote: ... falciparum asexual blood stages were cultured in human erythrocytes (National Blood Transfusion Service, London, United Kingdom) and complete medium (CM) consisting of RPMI-1640 medium (Life Technologies, California, United States) supplemented with 0.5% AlbuMAX type II (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genetics 2019Quote: ... chronic myelogenous leukemia cells and six independent transfections in SH-SY5Y (ATCC#CRL-2266) human neuroblastoma cells in two batches by lipofectamine3000 (Thermofisher Scientific cat. no L3000008). We then extracted DNA and RNA and performed 50 bp non-paired end sequencing reading from the 3’end ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 48h at 4°C in the same solution with mouse anti-NeuN (1:2000; Chemicon; MAB377, Temecula, CA, USA) and anti-human PHF-Tau antibody clone AT8 (1:2000, MN1020, Thermo Scientific, Waltham, MA, USA); for the sake of clarity ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...