Labshake search
Citations for Cell Signaling Technology :
1151 - 1200 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
-
bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
-
bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
-
bioRxiv - Physiology 2023Quote: ... Neurons were then incubated overnight at 4°C in 1% bovine serum albumin in PBS with pCREB (Ser133) (87G3) Rabbit mAb (1:333, Cell Signaling, Cat No. #9198). Neurons were then thrice rinsed with PBS ...
-
bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
-
bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
-
bioRxiv - Neuroscience 2023Quote: ... was incubated overnight at 4°C followed by corresponding horseradish peroxidase (HRP)-linked anti-mouse (Cell Signaling Technology, 7076V, 1:2000 dilution in TBST) or anti-rabbit (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 48 hours at 4°C in PBS-T-containing rabbit anti-IGF1R β antibody (1:1000; Cell signaling, Cat#9750), which has been previously validated [46] ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
-
bioRxiv - Microbiology 2024Quote: ... These cells were stained with 50 µM DAPI and incubated at 4 °C overnight in PBS-BSA containing rabbit anti-RNF2 IgG (clone D22F2, Cell Signaling Technology, Denver, MA) and mouse anti-NS5A IgG (clone 9E10 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissues were then incubated overnight at 4°C with anti-DeltaFosB primary antibody (DeltaFOSB; rabbit; Cell Signaling Technology, ref. 22509; 1:1000 dilution) or anti-Fos antibody (c-Fos ...
-
bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatants (Triton X-100 insoluble fraction) were collected and analyzed on 4-20% SDS-PAGE with anti-ubiquitin (Cell Signaling Technologies P4D1, #3936) and anti-Ref(2)P/p62 (Abcam #178840 ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were incubated for 48 hours at 4°C in PBS-T-containing rabbit anti-IGF1R β antibody (1:1000; Cell signaling, Cat#9750). After three washes in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were then incubated overnight at 4 °C with primary antibodies diluted in the blocking solution: anti-TRKB (1:500, Cell Signaling Technology, Cat# 4607S) and anti-GAPDH (1:5000 ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were incubated with primary antibody diluted in PBS overnight at 4°C at the following concentrations: Rabbit anti CD206 (Cell Signaling 24595S, 1:200), Rabbit anti LYVE-1 (Abcam ab218535 ...
-
bioRxiv - Immunology 2024Quote: ... An equal volume of 2× STOP Buffer (Buffer 2 plus 20 µM EDTA plus 4 µM EGTA) and 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. no. 29987) were added ...
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated at 4°C overnight with primary antibodies rabbit anti-RIPK2 (1:1000; Cat. No. 4142; Cell Signaling Technology, Danvers, MA), rabbit anti-iNOS (1:1000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated overnight at 4°C with the following primary antibodies: anti-mouse cleaved Caspase-3 (Cell Signaling, 9661S; 1:100 dilution) and anti-rat Ki67 (eBioscience 41-5698-80 ...
-
bioRxiv - Cancer Biology 2024Quote: ... incubated overnight at 4°C with 1:100 anti-nSMase2 (sc-166637) and further processed with SignalStain® Boost IHC Detection Reagent (Cell Signaling Technology, #8125) and DAB Substrate Kit (CST #8059 ...
-
bioRxiv - Cell Biology 2020Quote: ... Transferred blots were blocked for 1 hour in 5% w/v non-fat dry milk in 1X TBST (Cell Signaling Cat# 9997). Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858) ...
-
bioRxiv - Cell Biology 2020Quote: For immunoblotting the following primary antibodies were used at a concentration of 1:1000 in 5 % milk-TBS-T: HA (Cell Signaling 3724S), HA (Biolegend ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
-
bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
-
bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...
-
bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
-
bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
-
bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
-
bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
-
bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
-
bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... or 5 million cells for histone modifications was sheared with SFX250 Sonifier (Branson) and immunoprecipitated with antibodies targeting H3K27ac (#8173, Cell signaling Technology), H3K4me1 (#5326 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
-
bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
-
bioRxiv - Developmental Biology 2021Quote: ... coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449}, H3K4me3 {Millipore 04-745} and H2AUb {Cell Signaling 8240S}) ...
-
bioRxiv - Immunology 2020Quote: ... cells were cytospun 5’ at 500 rpm and senescence-associated beta-glalactosidase activity measured as recommended by the manufacturer (Cell Signaling Technology) on a Zeiss-Axio inverted phase-contrast microscope ...
-
bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
-
bioRxiv - Cancer Biology 2021Quote: ... NCI-H522 and NCI-H1299 cells were treated with 5 μg/mL Poly I:C sodium salt (Cell Signaling Technologies Cat No. 61401) for 6 and 12 hours respectively.
-
bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...