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Citations for Cell Signaling Technology :
1051 - 1100 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 200,000 Bead-bound cells were incubated with primary antibody overnight at 4°C (1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173), 1:50 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with rabbit anti-c-fos primary antibody (1:500; Cell Signaling Technology, catalog no. 2250, USA) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C with the primary antibody diluted in blocking buffer or SignalStain® Antibody Diluent (cat. 8112, Cell Signaling Technology). Slides were incubated with biotinylated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... for one hour at room temperature before being incubated overnight at 4°C with the following primary antibodies: anti-phospho-p38 (1:1,000, #9211, Cell Signaling Technology, Billerica, MA); anti-p38 (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-HA antibody (Cell signaling technology, C29F4) and Sera-Mag SpeedBeads Protein A/G (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were incubated overnight at 4⁰ C with rabbit anti-c-Fos primary antibody (5348, 1:2000; Cell Signaling Technology, RRID: AB_10557109) diluted in 2% NHS in PBS-Tx.
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bioRxiv - Physiology 2024Quote: ... Protein content was measured using DC Assay and samples processed as above to probe for activating transcription 4 (ATF4) abundance (Cell Signaling cat. 11815) as a marker of ER stress.
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bioRxiv - Immunology 2024Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20 μM EDTA + 4 μM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification.
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bioRxiv - Cancer Biology 2024Quote: ... Then 300 µl of lysate was immunoprecipitated by rotating overnight at 4° C with rabbit anti-HA epitope (Cell Signaling #3724, 1:100) for exogenous cyclin B ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked and then incubated overnight at 4°C with the primary antibodies: Tubulin (Sigma, Cat.#T6074; Cell signaling, Cat.#2144), HA-tag (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000 ...
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bioRxiv - Bioengineering 2024Quote: ... for an hour with rocking at room temperature and were incubated with the following primary antibodies for overnight with rocking at 4 °C: anti-phosphoSRC(Tyr416) (1:1000, Cell Signaling Technology, #2101), anti-p110α (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with primary antibodies overnight at 4°C and then probed with secondary antibodies: horse anti-mouse IgG (Cell Signaling Technology #7076) or goat anti-rabbit IgG (Cell Signaling Technology #7074) ...
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bioRxiv - Cell Biology 2024Quote: ... cardiac myocytes were incubated with primary antibodies in blocking buffer at 4°C overnight as follows: P-S6 Ribosomal ProteinS235/2361:500 (cat# 2211, Cell signaling, Danvers, MA); RyR2 1:500 (cat# MA3-916 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with primary antibody in TBST overnight at 4°C: Phospho-Akt (Ser473) antibody (Cell Signaling Technology, #9271, 1:1000), Akt (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with methanol/acetone (50/50) and then stained intracellularly by incubating cells with rabbit antibody against p-(Y694) STAT-5 (D47E7) XP (Cell Signaling), mouse antibody against BCL-2 (100 ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were stained with polyclonal anti-TCF7L1 antibody raised in rabbit (1:100 for complete slides, 1:25 for TMAs; 28835 clone D15G11 lot 5, Cell Signaling). For TMA analysis ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in 5% milk in TBST) were used for western blotting: E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), N-cadherin (#13116 ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... and probed with the following antibodies diluted in 5% skim milk/PBS (w/v): anti-HA (1:4000, clone C29F4, Cell Signaling), anti-cMyc (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with either 5% skimmed milk or 5% bovine-serum albumin (BSA) and incubated with dilution of respective antibodies as per manufacturer’s instructions (Cell Signaling technologies). The membrane was washed ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cancer Biology 2022Quote: ... De-identified FFPE tumor specimens were cut into 5 μm sections and IHC performed as described above to stain for human EZH2 (5246, Cell Signaling) and NKp46 (AF1850 ...
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bioRxiv - Developmental Biology 2022Quote: ... antigens were retrieved by boiling slides in citrate buffer (pH 6.0) for 1 min and pSMAD1/5 Ab (Cell Signaling, 41D10) was added overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... fresh frozen OCT-embedded embryo lung sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...