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Citations for Cell Signaling Technology :
851 - 900 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Thermo Fischer Scientific MA5-11690) overnight at 4°C and then incubated with goat anti-rabbit HRP linked IgG (1:10000, Cell signaling). Membranes were visualized using a ChemiDoc MP (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Drosophila brains were fixed in 4% PFA for 10 min and prepared for staining according to the manufacturer’s protocol (Cell Signaling Technology). Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner ...
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bioRxiv - Developmental Biology 2020Quote: ... and antibody (1.2 ug of anti-H3K4me1: Diagenode, C15410194, Lot A1862D or 4 uL anti-H3K4me2: Cell Signaling #9725, Lot 9) was added per 0.6 mL-tube ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
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bioRxiv - Cell Biology 2024Quote: ... solution one hour at room temperature and subsequently incubated overnight 4°C with primary antibodies: anti-S6 (1:10,000; 5G10 monoclonal rabbit; Cell Signaling 2217), anti-Phospho-S6 (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... we used a rabbit monoclonal anti-HA antibody (1:500 in 0.3 % Triton in PBS; 48 h incubation at 4 °C; #3724, Cell Signaling Technology), followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor pieces were fixed in 4% PFA prior to paraffin embedding and then stained with GR (1:1000, Cell Signaling, D6H2L) on a bond III automated stainer (Leica Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C with the primary c-Fos antibody (1:1000, Rabbit, Cell Signaling #2250). After 3 rinses with PBS for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blocked in 5% dry milk in TBS-T for 30 min and incubated overnight at 4°C with primary antibodies against: rabbit V5 at 1:1,000 (Cell Signaling Technology), mouse vinculin at 1:1,000 (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with primary antibodies at 4°C overnight: anti-HIF-1α (1:1000, Cat #36169, Cell Signaling Technology, USA) or anti-β-Actin (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... 400 μl of clean sample was then incubated overnight at 4 °C on gentle shaking with 20 μl of either anti-MYC-agarose beads (3400, Cell Signaling) or anti-HA-agarose beads (3956 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3x with PBST and incubated overnight at 4°C under agitation with rabbit anti-phosphorylated TRKB (Cell Signaling, either #4168 against pY816 or #4619 against pY515 ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation in 5% FBS in PBS for 16 h at 4°C with primary antibodies (1:1,000) to the following: LAMP1 (Cell Signaling, 9091), p62 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-c-Fos (1:3000, RRID:AB_2247211, Cell Signaling Technology, USA), mouse anti-NeuN (1:800 ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4 °C with the following primary antibodies diluted in Superblock/0.1% Tween 20: p-JNK (1:1000; Cell Signaling Technologies), total-JNK(1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.1% Tween 20 (TBST) and probed with primary antibodies overnight at 4 °C using rabbit NLRP3 (1:1000, D4D8T-15101, Cell Signaling), mouse caspase 1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with the different antibodies overnight at 4 °C (anti-hSYK #2710, anti-hPhospho-SYK #2712 from Cell Signaling were used according to manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2024Quote: Aortae were perfusion-fixed in situ with 4% paraformaldehyde (overnight) prior to staining with primary beta-catenin antibody (Cell signaling: 9562) and fluorophore-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in Tris-buffered saline with Tween-20 (0.1%), and subsequently incubated (overnight, 4 °C) with primary antibodies for p-Akt (S473) (1:1000, Rabbit mAb, Cell Signaling, 4060S), Akt (pan ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated overnight at 4° C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 to 250 µg of chromatin were incubated overnight on a rotator at 4°C with Ezh2 antibody (Cell Signaling Technology). On the next day ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4 % PFA at passage 1 and stained for the endothelial markers CD31 (Cell Signaling 3528S, 1:800), VE-cadherin (2500S ...
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bioRxiv - Genomics 2024Quote: ... 0.05% Tween-20) and incubated overnight at 4°C with primary antibodies: rabbit anti-HIS tag (D3I10) mAb (12698, Cell Signaling); mouse anti-β-tubulin (clone E7 ...
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bioRxiv - Cell Biology 2024Quote: ... rocking at 4°C (1:1000 rabbit anti-GFP and 1:500 mouse anti-EEA1, or 1:1000 mouse anti-GFP (Cell Signaling) and 1:1000 rabbit anti-GM130 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h at room temperature and then incubated overnight at +4°C with primary antibody: anti-Snai1 (Cat# 3879, Cell Signaling), anti-STIM1 (Cat# 4119 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then blocked in 5% normal goat serum/0.1% saponin for 1 hr at room temperature and incubated with combinations of the indicated primary antibodies overnight at 4°C: rabbit monoclonal anti-ECAD (clone 24E10; 1:1,000; Cell Signaling Technology), mouse monoclonal anti-HLA-G (clone 4H84 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μg of digested chromatin samples was treated with antibodies against rabbit IgG (1:100; Cell Signaling Technology, 2729) or NKX2.1 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5% milk powder in TBST for 1 h and probed with SETD2 (Cell signaling, 80290) 1:1000 overnight at 4°C with agitation ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-vimentin antibody (clone D21H3, Cell Signaling Technologies, (1:500 dilution in 5% milk/TBS-T) and with anti-GAPDH antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with 5% powdered milk in buffer and probed with pan-SynGAP (1:1,000, #5539, Cell Signaling) or SynGAP-α2 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then incubated with 1× TBST containing 5% milk and either anti-RPS6KB1 (Cell Signaling Technology, 2940S) or anti-DNAJC3 (Cell Signaling Technology ...
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bioRxiv - Pathology 2022Quote: ... Membranes were stained with Ponceau-S and blocked for 30 minutes with 5% nonfat dry milk (Cell signaling, 9999) or 5% BSA in Blotto buffer (0.15M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies were used: Phospho-Smad1/5 (Ser463/465) (41D10) (1:300, Rabbit, Cell Signaling Technology #9516S), P-Histone H3 (1:500 ...