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Citations for Cell Signaling Technology :
1101 - 1150 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was then incubated with 5 μg of Anti-FLAG Antibody (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling) and 50 μL of protein G beads at 4 ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies were diluted 1:1000 in 5% milk/TBST (FGFR1: D8E4; GAPDH HRP-conjugated: 14C10; Cell Signaling, Danvers, MA, USA). The membranes were incubated with antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked for 1 h in 5% non-fat milk in 1X TBST at room temperature and incubated with primary antibodies against KEAP1 (#8047, Cell Signaling), NQO1 (#11451-1-AP ...
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bioRxiv - Physiology 2024Quote: ... C2C12’s were replated and allowed 24 hours to recover after which the labeled C2C12 myoblasts were induced for apoptosis using 5 µM Staurosporin (cat. no.: 9953S, Cell Signaling) for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Physiology 2024Quote: ... Triton X-100 CD31+ and CD31-cell lysates (5-15 µg/lane) were analyzed by western blot for proteins and eNOS (#32027; 1:500; Cell Signaling) and GAPDH (#97166 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were washed three times with PBS-T for 5 minutes each.The wells were then incubated with Mouse Anti-Myc antibody (9B11, Cell Signaling Technology) diluted 1:3000 in PBS-T with 5% BSA for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with primary antibody anti-phospho-histone H2A.X (γH2AX) (#2577; Cell Signaling. 1:200 in 5% BSA in PBS), for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... Knockdown efficiency was validated by immunoblotting with pannexin-1 primary antibody (Cell Signaling Technologies, 91137, 1:1000 in 5% BSA-TBST).
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3 5-minute washes by 1x TBST and probing with horseradish peroxidase conjugated secondary antibodies (Cell Signaling, 7074, 7076) for 1-1.5 hours at 25°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then stained overnight with primary antibodies in blocking buffer (5% goat serum or SignalStain antibody diluent, Cell Signaling Technology, cat#8112). For non-CST primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated overnight at 4°C in the primary antibodies against c-Fos (1:6400; catalog #2250S; Cell Signaling Technology, Danvers, MA), and then incubated with the secondary Alexa Fluor® 448 (1:800 ...
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bioRxiv - Cell Biology 2021Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then rinsed and blocked with 5% BSA for 1hr prior to overnight incubation (4°C) with monoclonal rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)-XP (Cell signaling, 4511T, 1:1,000) or monoclonal rabbit anti-p38 MAPK (D13E1 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently incubated overnight at 4 °C with the primary anti-ADPr-antibody (diluted 1:10’000 in TBS-T; Cell Signaling Technology, Leiden, Netherlands25, 26). Membranes were washed 3x for 5 min with TBS-T and incubated IRDye goat-anti-rabbit IgG secondary antibody (diluted 1:15’000 in TBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were subsequently incubated overnight at 4° C with primary anti-phosphorylated ERK antibody (anti-pERK, Cell Signaling, Cat # 4370, RRID: AB_2315112, 1:400). After primary antibody incubation and PBS wash ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in rabbit anti-c-fos(1:1000 dilution, 2250T Rabbit mAb, Cell Signaling Technolog, Danvers, Massachusetts, USA) primary antibody or mouse anti-TPH2 (1:100 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a variety of primary antibodies including anti-MFN2 (Cell Signaling Technology; diluted 1:200), anti-TRA-1-81 (diluted 1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Cell Biology 2023Quote: ... and an equivalent of 1 µg of DNA-normalized DPCs was resolved using SDS-acrylamide gradient gel with the addition of 4 M urea (Cell Signaling, #38650, 1: 1000). DNA detection was performed by applying 2 ng of the sample collected for Pico Green detection to a nylon membrane and detecting with the α-dsDNA antibody (abcam ab27156 ...
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bioRxiv - Microbiology 2023Quote: ... and the remaining volume was rotated overnight at 4 °C with 45 μl of anti-C-myc magnetic beads from Cell Signaling Technology (Cat. 5698) or Pierce (Cat ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated overnight at 4°C with different primary antibodies in TBS-T against LEF-1 (1:500, Antibody# 2230, Cell Signaling Technology, MA, USA), and β-actin (1:30000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution), EAAT3 antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...