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Citations for Cell Signaling Technology :
951 - 1000 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 hour at room temperature and then incubated overnight at 4°C with antibodies recognizing human TIM4 (Cell signaling technology, #75484T ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760, 1:1000; IMPDH2, Abcam ab129165, 1:1000; S6, Santa Cruz sc74459, 1:1000; p-S6, Cell signaling 5364 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... then stained at 4°C overnight with various combinations of the following antibodies: rabbit anti-NeuN (1:1000, Cell Signaling Technologies, 12943S), rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and then incubated overnight at 4°C with anti-p-AKT (Ser473) antibody (1:1000 dilution, RRID: AB_2315049, Cell Signaling Technology 4060T) and anti-H3 antibody (internal control ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with various antibodies: rabbit anti-pan ubiquitin (1:1,000, #3933S; Cell Signaling Technology, Danvers, MA), rabbit anti-K48-linkage specific polyUb (D9D5 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with a rabbit polyclonal anti‐pERK antibody (Cell Signaling; 9101L, lot #30, 1:1000), and mouse anti-NF200 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...
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bioRxiv - Biochemistry 2022Quote: ... 30-50 μg of chromatin was used per sample and incubated with antibodies in a total volume of 600 μL overnight at 4° C with H3K27me3 antibody (Cell Signaling, 9733) or H4K20me0 antibody (Abcam ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or Phospho-CD19 (Tyr531 ...
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bioRxiv - Molecular Biology 2023Quote: ... over night at 4 °C with a dilution of 1:200 and subsequent incubation with a HRP-linked secondary anti-mouse antibody (Cell Signaling #7076) for 2h at RT with a 1:5000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 immunohistochemistry was performed on 4-μm FFPE tumor tissue sections using a standard PD-L1 antibody (E1L3N; dilution 1:100, Cell Signaling Technologies) validated in the clinical laboratory at the study institution ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with 5% milk in TBST for 1 h at room temperature and then probed overnight at 4°C with pStat1 (1:1000; Cell Signaling #9167S), β-actin (1:500 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were incubated overnight at 4°C with the primary antibody against VE-Cad (rabbit mAb, D87F2, Cell Signaling Technology, 1:200) diluted in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Physiology 2023Quote: ... for 1 hour and then incubated overnight at 4°C with primary antibodies p-S6 (Ser240/244, 1:1000, Cell Signaling, #2215), S6 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from FT282 and KURAMOCHI were bound to Concalavin A beads and incubated overnight at 4℃ with primary antibody for H3K27me3 (Cell Signaling, RRID:AB_2798370), H3K27ac (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated with one of the following primary antibodies in antibody dilution buffer at 4°C overnight: anti-YAP1 (1:2,000 dilution; DBH1X; Cell Signaling, Danvers, MA), anti-CDX2 (1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling, #3724, 1:500 dilution), in combination with one of four primary guinea pig antibodies against synaptic markers ...
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bioRxiv - Neuroscience 2023Quote: ... in PBST and then incubated for 15 hrs at 4 °C in primary anti-c-Fos (rabbit monoclonal, 1:2000, Cell Signaling 2250) diluted in PBST containing 4% NGS ...
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bioRxiv - Biophysics 2023Quote: ... The membranes were incubated overnight at 4 °C with primary antibodies against VDAC1 and β-Actin (1:5000, #2146; Cell Signaling Technology) after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were incubated overnight in agitation at 4°C with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370; Cell Signaling Technology) in a dilution 1:1000 with PFBS ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were incubated overnight at 4°C with rabbit anti-c-fos (1:500, Cell Signaling Technology, catalog no. 2250S). Sections were then washed with PBS ...
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bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
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bioRxiv - Immunology 2024Quote: ... The cells were stained overnight at 4 °C with the following primary antibodies: anti-NFAT1 (1:200, cat. 4389, Cell Signaling Technology), anti-NFAT2 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: Membranes were subsequently incubated overnight at 4°C with the following primary antibodies: rabbit anti-GAPDH (1:1,000, Cell Signaling Technology #2118) or rabbit anti-HTT ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in 4°C overnight in primary antibody for c-Fos (1:750, Rabbit mAb Cell Signaling Technology, cat #2250). The following day ...
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bioRxiv - Pathology 2024Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-STING (1:1000; #13647, Cell Signaling; Danvers, MA, USA), rabbit anti-TBK1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were blocked with 0.2% bovine serum albumin and incubated overnight at 4°C with the anti-EEA (1:200; Cell Signaling Technology, 3288) or anti-LAMP1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then incubated under soft agitation overnight at 4°C with the primary antibodies for DNMT1 (Cell Signaling Technology, 5032) and β-actin (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Samples containing 10 μg of DNA were then incubated overnight at 4°C with either anti-RNA pol II antibody (mouse anti-Rpb1 CTD, Cell Signaling, 2629S), mouse anti-SP1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2024Quote: ... overnight at 4 °C and then with horseradish peroxidase (HRP)-conjugated anti-mouse immunoglobulin G (IgG) antibody (7076S; Cell Signaling Technology, USA) for 1 hour at room temperature ...