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Citations for Cell Signaling Technology :
901 - 950 of 1331 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) (Cell Signaling Technology, 4668S, 1:2500 in 5% BSA); Rabbit polyclonal antibodies to p38 MAPK (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the MCF10A cell line by chronic stimulation with 5 ng/ml TGFβ1 (Cell Signaling Technology) for eight days22 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with anti-NTMT1 (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #13432) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% milk and probed using either 1:1000 antiV5 antibody (Cell Signaling Technology, 13202) or 1:10000 GAPDH as a loading control (Proteintech 60004-1) ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% skimmed milk in Tris-Buffered Saline with Tween 20 (TBST; Cell Signaling Technology) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first incubated with blocking and permeabilization buffer (1X PBS / 5% normal goat serum (Cell Signaling, cat #5425) / 0.3% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... 1ml (5*106 cells) was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blotted overnight with primary antibodies in TBS-T containing 5% BSA (Cell signaling antibodies; Stat1 (9172), phospho-Stat1-Tyr701-(7649) ...
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bioRxiv - Cell Biology 2023Quote: Cullin-RING E3 ubiquitin ligases were inhibited by addition of 5 μM MLN4924 (Cell Signaling Technology, catalog no.: 85923) and the 26S proteasome was blocked with 10 μM MG132 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% BSA TBST and incubated in the primary antbodies anti-INSR (Cell Signaling Technology, #3025), anti-TdTomato (SICGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rinsed 3 times with PBS for 5 minutes before adding fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:2000 in PBS containing 1% BSA and 0.1% Triton-X at 37°C for 25 minutes ...
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bioRxiv - Immunology 2024Quote: ... blocked in BSA 5% in TBS-Tween and then stained with antibodies (488-conjugated rabbit anti-Iba1, Cell Signaling, #20825 ...
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bioRxiv - Immunology 2024Quote: ... and subsequently blocked with 5% non-fat milk or BSA and probed with primary antibodies from Cell Signaling Technologies against phospho-proteins p-Akt (Ser473 ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking tissue with 5% swine serum (Jackson Immuno Research, # 014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary HRP-linked antibody was incubated (anti-mouse, Cell Signaling Technology, 1:3000 in 5% milk in PBS-T) for 1h at RT ...
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bioRxiv - Bioengineering 2020Quote: ... microchannels were incubated overnight at 4°C with anti-vascular endothelial-cadherin (VE-cadherin) and anti-epithelial cadherin (E-cadherin) (Cell Signaling Technologies) and primary antibodies were diluted with blocking buffer for HUVECs and BeWo cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... were incubated overnight at 4 degrees Celcius in a 1:1000 dilution with the following primary antibodies: anti-TP53 (Cell Signaling, 2524), anti-BRCA1 (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocked using 5% milk in TBST for 1 hour followed by primary antibody incubation at 4°C for overnight using the following antibodies at the appropriate dilutions– LC3 (1:1000, #2775, Cell Signaling Technologies), Atg5 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 100 µl/well of diluted H3K9ac on a shaker at 4°C overnight and then treated with Alexa Fluor 488 conjugate secondary antibody (Cell Signaling #4412) at room temperature ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... run using standard Ventana reagents a and Vector secondaries for staining with the TrkB.T1 SPEH1_D12 scFv-Fc fusion (Pattwell et al. 2020b) at 1:500 and PTEN (Cell Signaling #9188, Lot #4) at 1:100 or TrkB (kinase specific against amino acid 810 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with 1:5 home-made anti-p53 (overnight at +4°C) and 1:100,000 anti-Lamin B1 (1 hr at room temperature) (Cell Signaling Technology, #12586). As secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight incubation at 4°C with the following primary antibodies: 4EBP1 (1:1,000, Cell Signaling Technology, Cambridge, UK #9644), MYCN (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...