Labshake search
Citations for Diagenode :
301 - 350 of 2645 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
-
bioRxiv - Molecular Biology 2023Quote: ... Unloaded Tn5 enzyme can be purchased from Diagenode (Cat:C01070010-10) and all sequences used for loading Tn5 are contained in Supp ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 20 cycles (30 sec on/30 sec off, high-intensity) in a Bioruptor NextGen (Diagenode). Chromatin and DNA samples were processed in DNA LoBind tubes from here on (Eppendorf #2023-04-28 ...
-
bioRxiv - Molecular Biology 2023Quote: ... lysed using a Bioruptor sonication device (Diagenode) (settings ...
-
bioRxiv - Microbiology 2023Quote: ... followed by six cycles of 30 s of sonication (Bioruptor Pico, Diagenode, USA). Protein concentration was measured using the BCA assay (Thermo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were sonicated using a Bioruptor Plus from Diagenode (15 cycles of 30 s). The protein concentration was measured using a tryptophan assay ...
-
bioRxiv - Genetics 2023Quote: ... coupled to 30µL beads was added to 400µL soluble chromatin (Diagenode H3K9me3 antibody was used in Figure 2E only ...
-
bioRxiv - Genetics 2023Quote: ... For the H3K9me3 antibody (C15410193, Diagenode) preincubation was done with Streptavidin beads ...
-
bioRxiv - Genetics 2023Quote: ... Pellets were resuspended in 1ml lysis buffer + 0.1% SDS + cOmplete protease inhibitor and chromatin was sheared by sonication using a Bioruptor (Diagenode (Seraing, Belgium), 8 cycles of 30sec ON/45sec OFF) ...
-
bioRxiv - Genomics 2023Quote: ... H3K27Ac ChIP (Diagenode, C15410174) was performed as previously described (78) ...
-
bioRxiv - Genomics 2023Quote: ... Samples were sonicated with a Bioruptor Pico (Diagenode) for 18 cycles of 30 sec ON and 30 sec OFF intervals ...
-
bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 uL Tagmentase (Tn5 transposase - unloaded, Diagenode) was mixed with 20 uL annealed oligos and incubated on a thermomixer at 350 rpm ...
-
bioRxiv - Molecular Biology 2023Quote: ... 13.75 uL N7-loaded Tn5 (Diagenode) was mixed with 550 uL TD buffer and 5 uL of this mixture was added to each well ...
-
bioRxiv - Genomics 2023Quote: ... and subjected to gentle sonication using a Bioruptor (Diagenode) at 4°C to generate ChIP material ...
-
bioRxiv - Genetics 2023Quote: ... third instar larval wing discs were prepared by boiling samples for 10 minutes in Laemmli SDS-PAGE loading buffer followed by sonication using the Bioruptor Pico sonication system (Diagenode) for 10 cycles (30 sec on ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using IPure kit v2 (Diagenode, Belgium) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
-
bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were sonicated for 2X15 minutes (30-second ON, 30-second OFF pulses) at maximum power in a Bioruptor® Sonication System (Diagenode), and then centrifuged for high speed for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... by sonication (Bioruptor, 10 cycles, 30s on/off, Diagenode, Belgium). Proteins were subsequently reduced by 10min incubation at 95°C and alkylated with 10 mM chloroacetamide for 30min at 37 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Antibodies against H3K27ac (Diagenode #C15410174, 1.2 μg), H3K4me3 (Diagenode #C15410003 ...
-
bioRxiv - Developmental Biology 2023Quote: ... H3K27me3 (Diagenode #C15410069, 1.5 μg), p63 (Santa Cruz #H129 ...
-
bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were recovered and chromatin extracted and sonicated (Bioruptor, Diagenode) to an average DNA length of 500 bp ...
-
bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was sonicated in an ultrasonic bath (Diagenode, Bioruptor pico) to an average length of 200–250 bp ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were lyzed and chromatin was sonicated using a Biorupto device (Diagenode) to obtain fragments with an average size ranging between 100–600 bp ...
-
bioRxiv - Developmental Biology 2023Quote: ... Fragmented cDNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 s off using a Bioruptor Plus Sonication System (Diagenode, Denville, NJ, United States). Protein was quantified by Bradford54 and samples were mineralized in trace metal grade nitric acid HNO3 and boiled at 100 °C.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...
-
bioRxiv - Microbiology 2023Quote: ... About 2 µg of genomic DNA was sheared by sonication with a Picoruptor (Diagenode, S.A., Belgium) instrument to a size of 300–400 bp in length ...
-
bioRxiv - Microbiology 2023Quote: ... 1 ug of genomic DNA was sheared by sonication using a Picorupter (Diagenode s.a., Liege, Belgium) to obtain fragments with an average size of 300 bp ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode). The RPFs were diluted in a final volume of 8 µl before the addition of 2 µl of Dephoshorylation Buffer ...
-
bioRxiv - Genomics 2023Quote: ... Sonicate tissue using Diagenode Bioruptor Pico (10 cycles; 30 seconds on/ 30 seconds off) using 1.5mL microtubes (Diagenode C30010016) with 300uL per tube (several rounds needed) ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by sonication in a Bioruptor machine (Diagenode) for 10 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
-
bioRxiv - Immunology 2023Quote: ... and sonicated on ice for 5-10 min using a Bioruptor® sonicator (Diagenode). The cell lysate was centrifuged at high speed (16,000 g ...
-
bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...
-
bioRxiv - Genetics 2023Quote: ... Chromatin was sonicated using a Bioruptor Pico (Diagenode) to an average size of 200-700 bp ...
-
bioRxiv - Biophysics 2023Quote: ... Mature fibers were subsequently fragmented on ice using a sonicator (Diagenode UCD-300 bath sonicator) set to high ...
-
bioRxiv - Plant Biology 2023Quote: ... pH 8.0 and protease inhibitor cocktail) and sheared into 200-500 bp fragments with a sonicator (Diagenode, Bioruptor Plus, Belgium). The sonicated chromatin complex was immunoprecipitated by anti-H2A.Z and isolated by binding to Dynabeads Protein A/G (26162 ...
-
bioRxiv - Cell Biology 2023Quote: ... and lysed by sonication at high power for 8 cycles (15 sec on, 60 sec off) using a Bioruptor (Diagenode). The lysates were collected after centrifugation at 13,000 rpm for 10 min at 4 °C and the supernatants were prepared for nuclear protein analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... The tissue was homogenized with an electric grinder and sonicated using a Bioruptor® (Diagenode). The released DNA fragments were diluted in IP dilution buffer (20 mM Tris-Cl ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.05% Digitonin) and tagmentation was conducted by adding Tn5 transposase (Diagenode, C01070012) at 1:20 and incubated at 37C for 30min ...
-
bioRxiv - Molecular Biology 2023Quote: ... and chromatin was sheared by sonication using a Bioruptor (Diagenode, 9 cycles of 30 s on/off). Extracts were incubated for 2 h at room temperature with anti-V5 agarose beads (Sigma) ...
-
bioRxiv - Molecular Biology 2023Quote: ... a rabbit anti-H3K27me3 antibody (1:100; Diagenode, C15410195) was used ...
-
bioRxiv - Genetics 2023Quote: ... Rabbit IgG (Diagenode, C15410206). For ATRX CUT&RUN experiment ...
-
bioRxiv - Genomics 2023Quote: ... DNA was fragmented using the Megaruptor (Diagenode) and again evaluated with pulse electrophoresis ...