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Citations for Diagenode :
501 - 550 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... gDNA was sheared to 8 kb with the MegaRuptor device (Diagenode) and 2.6 µg sheared gDNA was used for library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sheared using a Megaruptor (Diagenode), and sized on a Blue Pippin instrument (Sage Science ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ultra-long gDNA was sheared to 75 kb fragments with the MegaRuptor device (Diagenode) and 4-8 µg sheared gDNA was used for library preparation ...
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bioRxiv - Genomics 2024Quote: ... the immunoprecipitation (IP) and library preparation steps were performed following a modified µChIPmentation protocol (Diagenode, Supplementary table 2) using Diagenode antibodies for immunoprecipitation (30 ng of chromatin per IP ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared with Megaruptor 3 (Diagenode, B06010003 & E07010003) using settings 28/30 ...
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bioRxiv - Neuroscience 2024Quote: ... total hippocampal DNA was fragmented by sonication using a Bioruptor (Diagenode) to a mean size of approximately 250 bp ...
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bioRxiv - Neuroscience 2024Quote: ... transferred to bioruptor tubes and sonicated in a Bioruptor Plus (both Diagenode) for ten cycles (30s on ...
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bioRxiv - Genetics 2024Quote: Bulk tagmentation was performed using either commercial pre-assembled Tn5 transposase (Diagenode) or with home-made Tn5 transposases produced and assembled using previously published methods (Picelli et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10.0 μg of high molecular weight purified genomic DNA (final volume of 120 μl) was sheared using the Diagenode Megaruptor 3 instrument (Diagenode Inc., Denville, NJ, USA) and a two-cycle shear method at speed 33 followed by speed 34 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by sonication (13 cycles, 30 sec on, 1 min off, Diagenode). Approximately 25 μg of chromatin was immunoprecipitated with 2 μg of antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and 150 mM NaCl) prior to sonication using beads in a Bioruptor (Diagenode) for 20 cycles of 15 seconds on and 30 seconds off ...
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bioRxiv - Systems Biology 2024Quote: ... 50 mM TEAB (Supelco) and sonicated using BioruptorPico (Diagenode) for 30 cycles (30s on/off) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue suspensions were sonicated on a bioruptor (Diagenode) with 30 s on/30 s off for 5 cycles ...
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bioRxiv - Genomics 2024Quote: Long-read sequencing was facilitated by fragmenting genomic DNA using the Megaruptor 3 shearing kit (Diagenode SA, Seraing, Belgium). Fragments less than 5 kb were selectively removed using the AMPure PB beads size selection kit (Pacbio ...
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bioRxiv - Genomics 2024Quote: ... Approximately 10 kb sheared DNA by the Megaruptor 3 system (Diagenode, Belgium; Cat. #B06010003) was used for removal of single-strand overhangs at 37°C for 15 minutes ...
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bioRxiv - Genomics 2024Quote: ... prior to shearing on a Megaruptor 2 (Diagenode, Denville, NJ), at 20kb setting.
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bioRxiv - Genomics 2024Quote: ... and anti-H3K27me3 (Diagenode, Cat. C15410069). The most commonly used and optimized CUT&RUN protocol (1 ...
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bioRxiv - Genomics 2024Quote: ... washed and pre-incubated with 2 µg of primary antibody (CTCF: Diagenode, C15410210-50 ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µg genomic DNA was sheared to a target fragment size of 19 kb using a Megaruptor 2 device (Diagenode, Denville, USA). In order to remove small fragments (<8kb) ...
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bioRxiv - Immunology 2024Quote: ... by ultrasound homogenizer (Bioruptor, Diagenode) for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... H3K27ac ChIP-seq with two H3K27ac antibodies (Active Motif, 39133; Diagenode, C15210016) was conducted as described previously 27 ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared by Diagenode, according to an optimized protocol on frozen tissues (Corces et al ...
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bioRxiv - Genomics 2024Quote: ... and sheared by Diagenode Biorupter Pico (EZ mode ...
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bioRxiv - Genomics 2024Quote: ... chromatin was fragmented using Bioruptor Plus sonicator (Diagenode) for 40 cycles (30 s on/30 s off on high setting at 4 °C) ...
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bioRxiv - Immunology 2024Quote: ... pA-Tn5 was sourced pre-loaded (C01070001, Diagenode). Primary antibody incubation occurred for 1 hr at RT with nutation and all other protocol steps as indicated in the native CUT&Tag protocol ...
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bioRxiv - Immunology 2024Quote: ... and chromatin was sheared using a Bioruptor (Diagenode) Sonicator (high power ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA was sheared with Megaruptor 3 (Diagenode, USA) to an average size of 15-20 kb ...
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bioRxiv - Systems Biology 2024Quote: ... unloaded Tn5 (2 mg/mL; Diagenode C01070010-20) was loaded with barcoded transposition primers and an initial transposition was carried out to validate the Tn5 concentration per cell required for transposition to yield fragment distributions with a mean fragment size around 800-1000bp ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SETD1A (Diagenode, CS-117-100), anti-SETD1B (Diagenode ...
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bioRxiv - Immunology 2024Quote: ... by sonication for 15 min (30 s on/off cycles) at 4 °C with high power in a Bioruptor device (Diagenode, Liège, Belgium). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were sonicated on a BIORUPTOR® PICO machine (Diagenode) at 4°C in the “low” setting for 5 min (30 sec on ...
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bioRxiv - Developmental Biology 2024Quote: ... Sonication was carried out with a Bioruptor® Standard apparatus (Diagenode) at high power for four series of 7 cycles (30 s on ...
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bioRxiv - Developmental Biology 2024Quote: ... or 10 µg of IgG antibody (Diagenode, C15410206) as control ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies were added as per the manufacturer’s suggested concentration for CUT&Tag (H3K27me3 (Diagenode, C15410195): 1 µg in 150 µl ...
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bioRxiv - Developmental Biology 2024Quote: ... H3K4me3 (Diagenode, C1541003): 0.5 µg in 150 µl ...
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bioRxiv - Developmental Biology 2024Quote: ... Library amplification was performed using NEBNext High Fidelity 2X PCR Mix with primers indexed with primers compatible with Nextera adaptors from Diagenode. 150 bp paired-end sequencing was carried out by Novogene (UK).
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bioRxiv - Developmental Biology 2024Quote: ... anti-HDAC1 (Diagenode, C15410325, 1:1000), anti-HDAC2 (Diagenode ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HDAC2 (Diagenode, C15200201, 1:1000), secondary goat anti-rabbit IgG (LICORbio ...
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bioRxiv - Neuroscience 2024Quote: ... and then sonicated in a water bath sonicator (Bioruptor Plus, Diagenode, Belgium). 5 μg of protein extracts were separated under denaturing conditions in a 12% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... Tn5 transposition reaction was done using 2.5 μl Tagmentase (Diagenode, #C01070012-30). From each sample ...
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bioRxiv - Genomics 2024Quote: ... The annealed adaptors were then diluted to 25 µM and incubated with equal volumes of Tn5 enzyme (Diagenode, Tagmentase C01070010-20) and glycerol at 37°C for 10 mins followed by 30 mins at 25°C ...
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bioRxiv - Genomics 2024Quote: ... DNA was sheared using a Bioruptor Pico (Diagenode) ultrasound device ...
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bioRxiv - Genomics 2024Quote: ... The samples were then transferred to fresh 1.5ml Diagenode sonication tubes and sonicated using a bioruptor (Diagenode) by sonicating 100ul at a time (4x30’’ON-30’’OFF) ...
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bioRxiv - Genomics 2024Quote: ... Chromatin was sheared using the Bioruptor pico (Diagenode) for 6 cycles (30 seconds on/30 seconds off ...
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bioRxiv - Genomics 2024Quote: ATAC-Seq experiments were performed using the ATAC-Seq kit from Diagenode (catalogue no. C01080002). Input material was between 100,000 to 130,000 cryopreserved PGCs (GFP-positive ...
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bioRxiv - Genomics 2024Quote: ... DNA was sheared to 10-20 kb with a Megaruptor 3 sonicator (Diagenode, Denville, New Jersey, USA) and treated with SMRTbell cleanup beads (PacBio ...
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bioRxiv - Biochemistry 2024Quote: ... in alternating 30 s ’ON’ and 30 s ’OFF’ cycles at the highest intensity setting (Bioruptor, Diagenode). Three volumes of B-PER™ bacterial protein extraction reagent (Thermo Scientific™ ...
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bioRxiv - Biochemistry 2024Quote: ... in alternating 30 s on-and 30 s off-cycles at highest intensity output (Bioruptor, Diagenode). Samples were diluted to 1 M urea and trypsin was added at an estimated 1:20 enzyme-to-protein ratio ...