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Citations for Diagenode :
101 - 150 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 2.5 mM sodium pyrophosphate) by sonication on a Bioruptor (Diagenode, Denville, NJ) with a five-minute method of 30s sonication followed by 30s off ...
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bioRxiv - Genomics 2024Quote: ... Chromatin was fragmented using a bioruptor Pico sonicator (Diagenode) to an average size of 200-600 bp and cleared chromatin was used for immunoprecipitation overnight at 4°C with protein A Dynabeads that were pre-coupled in 0.5% BSA with IgG or TOP1 antibodies ...
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bioRxiv - Genomics 2024Quote: ... proteinase inhibitor cocktail) and sonicated to fragments about 300 - 500 bp (Bioruptor, Diagenode). Dynabeads Protein A was washed twice with ChIP Buffer (10mM Tris-HCl pH7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... H3K27Ac antibody was added (Diagenode, C15410174), using a 2.5ul ...
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bioRxiv - Developmental Biology 2024Quote: ... The antibody used was ⍺-H3K27Ac (Diagenode C15410174) at a 1/500 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... These were sonicated to an average size of 200-500bp fragments on a Bioruptor Pico Sonicator (Diagenode) for 8 minutes 30 seconds ON/OFF at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Developmental Biology 2024Quote: ... libraries for sequencing were prepared using the MicroPlex library preparation kit v2 (C05010014, Diagenode). Libraries were purified using AMPure XP purification beads (A63880 ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of genomic DNA from each treatment condition was sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Genomics 2024Quote: ... Chromatin fragmentation was performed by sonicating samples 14 min (30sec on/off cycles) using a Bioruptor (Diagenode). The fragmented chromatin was incubated at 4°C overnight either with a rabbit polyclonal antibody against mTDG (Gallais et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was normalized (except for low quality samples) and mechanically fragmented to 300-600 bp fragments using a Bioruptor Pico (Diagenode; 15s ON, 90s OFF, eight cycles). Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... Lysates were sonicated for 4×15 minutes using a Bioruptor UCD-200 (Diagenode). Hybridization of probes was performed for 16 hours ...
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bioRxiv - Genomics 2024Quote: ... Chromatin was fragmented using a bioruptor Pico (Diagenode) or an E220 Focused-Ultrasonicator (Covaris ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode). For Morpho amathonte ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and anti-H3K36me3 (Diagenode, Premium, C15410192) antibodies ...
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bioRxiv - Genetics 2024Quote: ... sonicated in a Bioruptor Pico sonication device (Diagenode) 4 x 30 s in the ultra-high setting ...
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bioRxiv - Genomics 2024Quote: ... using the SX-8G IP-Star Automated System (Diagenode). ChIP samples were decrosslinked at 65°C for 4 hours ...
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bioRxiv - Genomics 2024Quote: ... lysed and subjected to sonication using a Bioruptor sonicator UCD-200 (Diagenode) to obtain chromatin fragments of 100–500 bp ...
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bioRxiv - Genomics 2024Quote: ... H3K4me1 (Diagenode – C15410194), and CTCF (Abcam – ab70303) ...
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bioRxiv - Genomics 2024Quote: ... Sheared chromatin was immunoprecipitated overnight with the following antibodies: H3K4me3 (Diagenode – C1540003-50), H3K27ac (Abcam – ab4729) ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared to a target size of 30 kb on a Megaruptor3 instrument (Diagenode) with either the DNAFluid+ needles at speed 45 for two cycles (cell and brain ...
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bioRxiv - Genomics 2024Quote: ... A total of 9.4μg of DNA was purified and then sheared at 20 kb using the Megaruptor system (Diagenode). Using SMRTbell® prep kit 3.0 ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared with Megaruptor 3 (Diagenode, B06010003 & E07010003) using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
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bioRxiv - Genomics 2024Quote: ... the µChipmentation kit for histones (Diagenode) was used for chromatin immunoprecipitation and ChIP-Seq library preparation (Supplementary table 2).
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bioRxiv - Genomics 2024Quote: ... incubated for 10 min on ice and sonicated with a Bioruptor Pico (Diagenode) for five cycles of 30s on and 30s off ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight genomic DNA was sheared mechanically on Megaruptor 3 (Diagenode) to generate 30 Kb fragments ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Microbiology 2024Quote: ... before and after sonication at 4°C for 15min in a Bioruptor® (Diagenode), to determine Chain (ChFU ...
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bioRxiv - Microbiology 2024Quote: ... but DNA was sonicated using a Bioruptor 300 (Diagenode) to obtain ∼500 bp fragments ...
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bioRxiv - Immunology 2024Quote: ... Lysates were sonicated with a BioRuptor UCD- 300 (Diagenode) for 13 cycles of 30 s on/30 s off ...
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bioRxiv - Immunology 2024Quote: ... or anti-H3K4me3 (C15410003, Diagenode). The immune complexes were washed ...
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bioRxiv - Immunology 2024Quote: ... or anti-H3K27me3 (C15410195, Diagenode) or anti-H3K4me3 (C15410003 ...
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bioRxiv - Immunology 2024Quote: ... anti-H3K27ac (C15410196, Diagenode), or anti-H3K27me3 (C15410195 ...
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bioRxiv - Immunology 2024Quote: ... H3K4me3 (rabbit, C15410003, Diagenode), and PE-conjugated survivin (mouse ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Genomics 2024Quote: ... followed by physical disruption with a sonicator (Bioruptor®, Diagenode, Belgium). The disrupted fecal suspension was vortexed and incubated at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Sonication was performed using the Bioruptor Pico (Diagenode) with 25 cycles of 30 seconds on ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then sonicated at high energy in a Bioruptor Plus (Diagenode). Sonication parameters to produce DNA fragments in the 200-600 bp range were optimized beforehand ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was fragmented to 200-400 bp using a Bioruptor (Diagenode) for 20-25 cycles (30 s on ...
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bioRxiv - Cancer Biology 2024Quote: ... and of antibodies 5 μg/ml IgG (Diagenode, C15410206), anti-CCL2 (Novus Biologicals ...