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Citations for Diagenode :
451 - 500 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Chromatin was prepared in FA buffer as described previously.55 DNA was fragmented using a Bioruptor® (Diagenode B01060010) with 25 cycles of 30 sec on and 30 sec off at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 5.5 μg of genomic DNA from each treatment condition was adjusted to 110μl (50ng/μl) and sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode) to shear genomic DNA to an average fragment size of 200 bp ...
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bioRxiv - Molecular Biology 2024Quote: Libraries were constructed using the D-Plex Small RNA-seq Kit from Illumina (Diagenode, C05030001) and the short RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... They were then sonicated by Bioruputer (Diagenode) on high for 10 cycles of 30 sec on ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin was sheared by Bioruptor Pico device (Diagenode) for 30 min (ultra-high power ...
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bioRxiv - Pathology 2024Quote: ... extracts were sonicated using the Bioruptor Pico (Diagenode, for 25 cycles at 8 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Plant Biology 2024Quote: ... We sheared the genomic DNA with the Megaruptor 2 device (Diagenode) to a target size of 20 kb to 25 kb ...
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bioRxiv - Plant Biology 2024Quote: ... The extracts were then distributed into 1.5 ml reaction tubes and sonicated in an ice bath four times for 30 seconds on a high setting using a Bioruptor UCD-200 (Diagenode) with 1.5 minutes breaks ...
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bioRxiv - Plant Biology 2024Quote: ... For mass spectrometry a sonication step was included (2 sessions on a Diagenode Bioruptor at 4°C at maximum amplitude with a 50% duty cycle for a total of 5 minutes) ...
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bioRxiv - Plant Biology 2024Quote: ... followed by nuclei isolation and chromatin shearing using a Bioruptor Pico (Diagenode). Chromatin was then immunoprecipitated overnight at 4°C with Anti-FLAG M2 antibody (5 μl/ChIP ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Plant Biology 2024Quote: ... was sonicated at 200 bp using BioRuptor Pico (Diagenode). The DNA library was prepared thereafter ...
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bioRxiv - Neuroscience 2024Quote: ATAC-seq libraries were generated using the ATAC-seq Kit from Diagenode (Diagenode, A Hologic Company) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode) before addition of 22.5 μL of H20 and 2.5 μL of Tn5 transposase ...
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bioRxiv - Neuroscience 2024Quote: Cortex nuclei were purified as above and collected by centrifugation (500 g; 5 min) for immediate use in ATAC Library preparation (Tagmentase–loaded; Diagenode). The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... The pellets were solubilized by resuspension in 6M Guanidine-HCl and sonication using a water-bath sonicator (Bioruptor, 5 min max output (Diagenode, Denville, NJ, USA)) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 % sodium deoxycholate (SDC) and MS-SAFE™ protease and phosphatase inhibitor using a Bioruptor ultrasonicator (Diagenode). Protein concentrations were measured using the BCA assay (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Fragmented cDNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... Sonication was conducted with the Bioruptor® Pico sonication device (Diagenode, B01060010) for 15 cycles (30 seconds ON/ 30 seconds OFF ...
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bioRxiv - Cell Biology 2024Quote: ... and chromatin was purified and sonicated using buffers and protocols from the IDeal ChIP-Seq Histone Kit (Diagenode, C01010051). Sonication was conducted with the Bioruptor® Pico sonication device (Diagenode ...
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bioRxiv - Cell Biology 2024Quote: ... decrosslinking and DNA purification were then conducted using the IDeal ChIP-Seq Histone Kit (Diagenode, C01010051). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue lysate was obtained using a Bioruptor sonication system (Diagenode) and then centrifugated at 2000 RPM for 5min ...
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bioRxiv - Neuroscience 2024Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Physiology 2024Quote: Library preparation was performed using the Premium RRBS kit (Diagenode). In brief ...
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bioRxiv - Physiology 2024Quote: ... samples were sonicated using a bioruptor twin (Diagenode) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... the seeds were sonicated using Bioruptor Pico® sonication device (Diagenode, Denville, NJ).
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bioRxiv - Cancer Biology 2024Quote: ... Lysates underwent 5 minutes of sonication with on/off intervals of 30 seconds using a Bioruptor (Diagenode), followed by centrifugation at 20,000 g for 10 minutes to retrieve the protein supernatant ...
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bioRxiv - Developmental Biology 2024Quote: ... and an iDeal ChIP–seq Kit for Transcription Factors (Diagenode) as previously described20 (see Supplemental Methods) ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor Diagenode, 15s ON/15s OFF for 15 min), and heating at 95°C for 10min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cuticule was punctured by sonication using Bioruptor (Diagenode) in 6x1.5mL tubes at a time (5 pulses of 5s ON/ 5s OFF in medium position) ...
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bioRxiv - Cancer Biology 2024Quote: ... H3K36me3 (Diagenode C15410192), H3K36me2 (abcam ab176921) ...
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bioRxiv - Cancer Biology 2024Quote: ... and H3K9me3 (Diagenode C15410056) using 100ng of MNase I digested chromatin per IP ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared using the MicroPlex Library Preparation Kit v3 (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4°C for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-seq was performed using ATAC-seq kit (cat# C01080002, Diagenode, Denville, NJ) with the CCHMC DNA sequencing core according to manufacturer’s instructions and analyzed as described previously.31 TaqMan gene expression analysis was conducted to validate some of the results from RNA-seq using probes mentioned above ...
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bioRxiv - Molecular Biology 2024Quote: ... Brains were subjected to 2 x 30 s of sonication with a Bioruptor (Diagenode), 20 min of incubation on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... utilizing SYBR Green Master Mix (Diagenode) on a BioRad Real-Time PCR instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... The WCE was sonicated using the Bioruptor (Diagenode) and samples cleared by centrifugation at 4°C 13000xg for 10’ ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated in a Bioruptor sonicator (Diagenode) for 3 cycles (30 s on / 30 s off cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were sonicated in a Bioruptor sonicator (Diagenode) for 30 min during 3 cycles of 10 min (30 s/on / 30 s off cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty-five million HCT116 TP53-/-cells per replicate and two biological replicates were prepared using Diagenode iDeal ChIP-seq kit for Transcription Factors (Diagenode #C01010170). Samples were fixed with 1% formaldehyde for 10 minutes followed by quenching with 250mM glycine ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were sonicated in a Biorupter (Diagenode) for 15 minutes on high ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were sonicated in a Biorupter (Diagenode) for 15 min on low ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 1% sodium deoxycholate (SDC) using sonication in a Bioruptor Pico (Diagenode). Protein concentrations were measured using the BCA assay (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... CHD1 (Diagenode, C15410334), and CHD2 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid film was resuspended by sonication in 100 µl of chloroform using a Bioruptor Pico (Diagenode, Denville, NJ). For TLC ...
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bioRxiv - Molecular Biology 2024Quote: ... then sonicated with a Bioruptor (Diagenode). After clearing the lysates by centrifugation at 20000g for 15min ...