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Citations for Diagenode :
551 - 600 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... the bacteria suspension was sonicated with a Bioruptor bath sonicator (Diagenode), with setting M ...
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bioRxiv - Genomics 2024Quote: ... and one of two U2OS libraries were prepared by first treating 3 μg of DNA with the Short Fragment Eliminator Kit (ONT) according to the manufacturer’s protocol before shearing with the DNA Fluid+ Kit on a Megaruptor 3 (Diagenode) to a mean size of approximately 50k bp ...
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bioRxiv - Genomics 2024Quote: ... Two-fold serial dilutions of the annealed Tn5 were prepared by diluting the stock Tn5 in Transposase dilution buffer (Diagenode, C01070011). To initiate transposition ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of each annealed A and B transposome were combined with 10 μl of unloaded Tagmentase (Diagenode, C01070010), briefly vortexed ...
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bioRxiv - Genomics 2024Quote: ... was added to 8.5 μl of transposition mix for multiplexed scATAC (comprising 7.5 μl of 2x Diagenode Tn5 reaction buffer and 1.5 μl of 10x Genomics 1x Nuclei Dilution Buffer ...
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bioRxiv - Genomics 2024Quote: ... Each pellet was then resuspended in 47.5 μl of transposition mix (comprising 25 μl of Diagenode 2x Tn5 reaction buffer ...
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bioRxiv - Genomics 2024Quote: ... the genomic DNA was sheared to 25kb using the Megaruptor 3 (Diagenode), as longer reads improve the ease of genome assembly ...
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bioRxiv - Genomics 2024Quote: ... DNA was first sheared at 10 ng.uL-1 by sonication for a single 30s interval at the lowest sonication intensity on a standard Bioruptor (Diagenode) to obtain a range of DNA fragments from 200 bp to >20 kb ...
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bioRxiv - Genomics 2024Quote: ... The Megarupter (Diagenode) was used for shearing and SageELF (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were synthesized using the Capture and Amplification by Tailing and Switching (Diagenode). Libraries were sequenced on the Illumina NovaSeq 6000 acquiring at least 10 million 50bp single-end reads per sample as described previously [20].
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bioRxiv - Genomics 2024Quote: ... and then were sonicated by using a BioRuptor sonicator (Diagenode). After centrifugation ...
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bioRxiv - Genomics 2024Quote: ... and the cross-linked chromatins were fragmented using a Bioruptor (Diagenode) with 16 cycles of 30 seconds on and off ...
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bioRxiv - Genomics 2024Quote: ... Fragmentation was performed via sonication (Diagenode Bioruptor) to produce 350 bp fragments ...
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bioRxiv - Biochemistry 2024Quote: ... The ultrasonic pyrolysis cycle included 10-sec ultrasonic pyrolysis (Diagenode, Seraing, Belgium) and 30-sec interval ...
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bioRxiv - Genomics 2024Quote: ... followed by a 20 min wash cycle using the provided wash buffers (Diagenode Immunoprecipitation Buffers ...
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bioRxiv - Genetics 2024Quote: ... We sheared the high molecular weight (HMW) DNA to achieve consistent and narrow size ranges to 20kb using the Megaruptor 3 Shearing kit (E07010003, Diagenode). Megaruptor 3 was performed using customized settings ...
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bioRxiv - Neuroscience 2024Quote: ... Thawed samples were sonicated (2 cycles [10 s], 30 s between cycles, high setting; Bioruptor Next Gen, Diagenode) and heated (+90 °C for 7 min) ...
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bioRxiv - Genetics 2024Quote: ... The nuclear fraction was sheared by sonication (Diagenode Pico), and treated with 4U TURBO-DNase ...
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bioRxiv - Genetics 2024Quote: ... 1% SDS) and sheared in a Bioruptor (Diagenode Pico) to obtain a mean fragment size of approximately 300 bp ...
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bioRxiv - Genomics 2024Quote: ... The HMW DNA was sheared to a target size of 30kb with the DNA Fluid + needles at speed 45 for two cycles on a Megaruptor3 instrument (Diagenode).
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bioRxiv - Genomics 2024Quote: ... 150mM NaCl) were added and samples sonicated using Bioruptor Plus sonicator (Diagenode) for two cycles (30 sec on/30 sec off on high setting at 4°C) ...
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bioRxiv - Genomics 2024Quote: ... sample shearing was executed using the Megaruptor 3 system (Diagenode s.a, Belgium) at a speed of 29-31 to achieve target insert lengths of 15-18 Kb ...
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bioRxiv - Genomics 2024Quote: ... H3K9me3 (Diagenode, C15410193), H3K9me2 (Active Motif ...
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bioRxiv - Genomics 2024Quote: ... H3K27me3 (Diagenode, C15410195), TOP2B (Novus Biological ...
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bioRxiv - Genomics 2024Quote: ... DNA were purified then sheared using the Megaruptor1 system (Diagenode) (Only the Te91 sample was not sheared) ...
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bioRxiv - Systems Biology 2024Quote: ... The samples in the SDC buffer were sonicated in high mode for 15 cycles (30 sec OFF, 30 sec ON) (Bioruptor® Plus; Diagenode). The samples were again heated at 95°C at 600 rpm in a thermoshaker for 45 min ...
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bioRxiv - Systems Biology 2024Quote: ... heat denatured at 95°C for 45 min at 600 rpm in a thermoshaker, sonicated in high mode for 30 cycles (30 sec OFF, 30 sec ON) (Bioruptor® Plus; Diagenode) and then precipitated using 80% acetone overnight in -20°C ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for four cycles of 10 minutes (30 seconds ON 30 seconds OFF) at high setting using a Bioruptor Plus sonication device (Diagenode). The resultant fragmented chromatin was centrifuged at 10.000 g for 10 minutes and the supernatant was collected ...
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bioRxiv - Bioengineering 2024Quote: ... after which they were sonicated on high for 5x 60 seconds on and 30 seconds off in a Bioruptor Pico sonication water bath (Diagenode) at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... was purchased from Diagenode and recombinant Tn5 protein was prepared in-house ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified DNA was fragmented using a Bioruptor standard UCD-200 (DIAGENODE) to obtain fragments from 500 to 1000 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 seconds “OFF”] on a Bioruptor Pico (Diagenode), and immunoprecipitated with anti-H2AK119ub1 antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... using the iDeal ChIP-seq Kit for Histones (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Chromatin ImmunpPrecipiation (ChIP) was performed using the Diagenode True Microchip kit (Diagenode), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was extracted and sheared to approximately 200 bp using the Diagenode Bioruptor Pico (Diagenode, Denville, NJ, USA) using 20 cycles (crosslink gold-fixed samples ...
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bioRxiv - Genetics 2024Quote: ... scapularis centromeric repeats by MeDIP: The Diagenode MagMeDip qPCR kit (Diagenode, C02010021) was used to quantify the DNA methylation status of ISR centromeric repeats of ticks infected and uninfected with B ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP reaction was performed with iDeal ChIP-seq kit for TFs (Diagenode, C01010055) with modifications of the manual detailed below ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5 µg H3K27ac antibody (Diagenode, C15410196) or 2.5 µg IgG antibody (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... Eluted and decross-linked DNA was purified with MicroChIP DiaPure columns (Diagenode, C03040001) and eluted in 30 µl of nuclease-free water ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2.5 µg IgG antibody (Diagenode, C15410206, RRID AB_2722554). Eluted and decross-linked DNA was purified with MicroChIP DiaPure columns (Diagenode ...
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bioRxiv - Biophysics 2024Quote: ... Anti-Cas9 antibody (Diagenode) was co-incubated with Protein A beads for ≥ 6 h prior to immunoprecipitation ...
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bioRxiv - Biophysics 2024Quote: ... cells were lysed by sonication with 15 cycles (15 s on/10 s off) using the Bioruptor Plus (Diagenode) at 4C ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were sonicated using a Bioruptor (Diagenode) at high intensity for 5 cycles (30 minutes on/30 minutes off ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were then sonicated for 15 cycles (30 s ON/30 s OFF) using the Bioruptor Pico (Diagenode). The resulting soluble cell lysates were diluted with dilution buffer (16.7 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... sonicated at high performance for 10 cycles (30 s on/off) using Bioruptor Plus Ultrasonicator (Diagenode), and spun down for 20 minutes at room temperature at 20000 g ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Biochemistry 2024Quote: ... The suspension was sonicated at 4°C with a Bioruptor (Diagenode, #B01020001) using conditions optimized to generate 200–500 bp chromatin fragments ...
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bioRxiv - Cell Biology 2024Quote: ... and sonicated on a Bioruptor Pico (Diagenode) for 15 cycles of 30 seconds on and 30 seconds off in 1.5 mL Diagenode tubes (Diagenode ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 cycles of 30 seconds on and 30 seconds off in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... and H3K27me3 (Diagenode; #C15410195; 1:200). The following secondary antibodies were used ...