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Citations for Diagenode :
351 - 400 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the seeds were sonicated using Bioruptor Pico® sonication device (Diagenode, Denville, NJ).
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bioRxiv - Cancer Biology 2024Quote: ... Lysates underwent 5 minutes of sonication with on/off intervals of 30 seconds using a Bioruptor (Diagenode), followed by centrifugation at 20,000 g for 10 minutes to retrieve the protein supernatant ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode) before addition of 22.5 μL of H20 and 2.5 μL of Tn5 transposase ...
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bioRxiv - Neuroscience 2024Quote: Cortex nuclei were purified as above and collected by centrifugation (500 g; 5 min) for immediate use in ATAC Library preparation (Tagmentase–loaded; Diagenode). The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... The pellets were solubilized by resuspension in 6M Guanidine-HCl and sonication using a water-bath sonicator (Bioruptor, 5 min max output (Diagenode, Denville, NJ, USA)) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 % sodium deoxycholate (SDC) and MS-SAFE™ protease and phosphatase inhibitor using a Bioruptor ultrasonicator (Diagenode). Protein concentrations were measured using the BCA assay (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Fragmented cDNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... samples were processed using a Bioruptor UCD200 (Diagenode) via 24 cycles of 15 s “ON “ followed by 15 s “OFF” at the high level to shear chromatin ...
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bioRxiv - Microbiology 2024Quote: ... and then sonicated (Diagenode Biorupter) for 15 cycles (30 seconds on ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were divided up into 250 uL aliquots and then sonicated with Bioruptor (Diagenode) with 30s on ...
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bioRxiv - Neuroscience 2024Quote: ... tissue lysate was extracted using a Bioruptor sonication system (Diagenode) and 2000 RPM centrifugation for 5 min ...
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bioRxiv - Genomics 2024Quote: ... 6.4 ug of HG008-T and 6.1 ug of HG008-N-P were sheared separately on a Megaruptor 3 (Diagenode, B06010003) to a target size of 15 to 20 kb ...
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bioRxiv - Microbiology 2024Quote: ... 1.2 μg of DNA for each sample was taken into fragmentation using Megaruptor 3.0 (Diagenode) with a speed of 31 and a final volume of 100 μl ...
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bioRxiv - Plant Biology 2024Quote: ... 200 μM PMSF) and sonicated in a Bioruptor Pico sonicator (Diagenode) (5 cycles of 30 s pulses at high intensity/30 s rest) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were lysed by sonication (Diagenode Bioruptor Plus), for 10 cycles (30sec on ...
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bioRxiv - Physiology 2024Quote: ... and 500 µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... or mouse IgG (Diagenode, C15400001) at 4 °C overnight ...
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bioRxiv - Genomics 2024Quote: Crosslinked tissue was then lysed using Chromatin Easy Shear Low SDS Kit (Diagenode #C01020013). Chromatin was sheared into 100–500 bp DNA fragments by sonication (Diagenode Bioruptor Pico ...
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bioRxiv - Genomics 2024Quote: A starting amount of 4-5 ug HMW gDNA was sheared to a target size of 20-30 kbp using a Megaruptor 3 instrument (Diagenode). The sheared DNA underwent size selection using a Pippin HT instrument (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... The chromatin was sonicated for 5 min using Bioruptor (Diagenode) with 30 s on / 30 s off intervals and high throughput settings.
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bioRxiv - Genomics 2024Quote: ... Chromatin was sheared into 100–500 bp DNA fragments by sonication (Diagenode Bioruptor Pico) for 8 cycles of 30s ON and 30s OFF ...
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bioRxiv - Genomics 2024Quote: DNA samples were homogenized using a Diagenode Megaruptor with the 3 DNAFluid+ Kit (E07020001, Diagenode) under the following conditions ...
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bioRxiv - Genomics 2024Quote: ... Shearing was performed with the Megaruptor shearing kit (E07010003, Diagenode) at speed 30 or 31 ...
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bioRxiv - Molecular Biology 2024Quote: ... They were subsequently sonicated for 4 cycles (30 sec on, 30 sec off) using the Bioruptor Pico (Diagenode) and centrifuged for 15 min at 14,000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... The cross-linked cells were then lysed and sonicated to obtain approximately 300 bp chromatin fragments with a Bioruptor Plus sonication device (Diagenode). For ChIP assays ...
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bioRxiv - Molecular Biology 2024Quote: ... Commercially available antibodies were used for immunoprecipitation: H3K9me3 polyclonal ab (C15410193, Diagenode) and RNA pol II 8WG16 mouse monoclonal ab (sc-56767 ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was sonicated to approximately one nucleosome using the Bioruptor Pico (Diagenode), 8 cycles of 30 s ON/30 s OFF in a 4°C water bath ...
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bioRxiv - Neuroscience 2024Quote: ... An equal volume of 2X lysis buffer was added to the homogenate and sonicated (Bioruptor Plus, Diagenode, Belgium) for 20 cycles (60 sec ON/30 sec OFF ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries prepared using the MicroPlex kit (Diagenode) were pooled and sequenced at a depth of approximately 30 M on an Illumina HiSeq 2500 at the Michigan State University (MSU ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1× protease inhibitors) using the Bioruptor Pico machine (Diagenode). The chromatin was precleared with Protein A Dynabeads (10002D ...
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bioRxiv - Plant Biology 2024Quote: ... IP was then performed using 4 µg of antibody to H3K27me3 (Diagenode, C15410069). Libraries were generated and sequenced as PE100 reads on a DNBSEQ platform at Beijing Genomics Institute (Hong Kong).
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bioRxiv - Plant Biology 2024Quote: ... For chromatin shearing using Bioruptor UCD-200 (Diagenode), nuclei were resuspended in 250 µl of lysis buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...
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bioRxiv - Neuroscience 2024Quote: ... The extracts were centrifuged for 10 min at 16,000g and supernatant was sonicated for 10 cycles with 30 sec on and 30 sec off per cycle on a Bioruptor Pico Sonicator (Diagenode). After sonication ...
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bioRxiv - Physiology 2024Quote: ... Cells in homogenized tissues were lysed by two freeze thaw cycles and subsequent sonication (Diagenode bioruptor). Protein concentration in the lysates was determined by BCA assay ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were sonicated for 10 cycles (30s on/ 30s off; Diagenode). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Samples were sonicated for 25 cycles (30 seconds on, 30 seconds off) using Bioruptor Pico (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... or 1 µg anti-H3K9me3 (Diagenode, Cat. No. C15500003 Lot No. 003) overnight on a tube rotator at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... sonicated in a Bioruptor sonication bath (Diagenode) (2 cycles of 30 s on – 30 s off ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated overnight at 4°C with rotation in wash buffer with rabbit anti-Chd1 (Diagenode 15410334, 1:50). Nuclei were incubated for 1 hour at room temperature with rotation with rabbit anti-H3K4me3 (Sigma-Aldrich 05-745R ...
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bioRxiv - Neuroscience 2024Quote: ... were subjected to 3 cycles of sonication (30sec ON/30sec OFF for 10 min, at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to the sonicated lysate to a final concentration of 1% ...
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bioRxiv - Neuroscience 2024Quote: ... on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus, Diagenode). The resultant chromatin was centrifuged at 12000 rpm for 30 minutes to remove any debris ...
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bioRxiv - Neuroscience 2024Quote: ... were subsequently subjected to 3 cycles of sonication (30s ON/30s OFF for 10 minutes at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to a final concentration of 1% ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-H3K27ac antibody (Diagenode C15410196). DNA sequencing libraries were prepared using the NEBNext Ultra II DNA Library prep kit using standard protocols ...
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... during 5 minutes then nuclei were sonicated in sonication Buffer (50mM TrisHCL pH8, 10mM EDTA, 0.3% SDS, 1M Na-Butyrate) with Bioruptor (Diagenode, B0102001 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s off using a Bioruptor Plus (Diagenode). Lysates were cleared via centrifugation at 21,000g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonication is performed using a Bioruptor Pico (Diagenode) with 5-10 cycles of 30 sec ON and 30 sec OFF ...
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bioRxiv - Physiology 2024Quote: ... Tissue homogenates were further processed to remove the nucleic acid traces (Diagenode SA ...
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bioRxiv - Molecular Biology 2024Quote: ... then sonicated with a Bioruptor (Diagenode). After clearing the lysates by centrifugation at 20000g for 15min ...