Labshake search
Citations for Diagenode :
351 - 400 of 2645 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
-
bioRxiv - Genomics 2023Quote: ... Approximately 10 μg chromatin was aliquoted and diluted to 500 μL of 1x ChIP Buffer and immunoprecipitated using 1.4 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode, Denville, NJ). In some cases ...
-
bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
-
bioRxiv - Genomics 2023Quote: The Bioruptor (Diagenode) was used to break the DNA into 300-500 bp using the following settings ...
-
bioRxiv - Molecular Biology 2023Quote: ChIP was performed with True ChIP-seq Kit (Diagenode: C01010132) following manufacturers guidelines with the exception that bead bound nuclei were fixed with 36.5% formaldehyde for 1 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were done with anti H3K4me1 and H3K4me3 antibodies (Diagenode: C15410194 & C15410003). DNA was purified using MicroChIP DiaPure columns (Diagenode ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using MicroChIP DiaPure columns (Diagenode: C03040001), and libraries were generated with the MicroPlex Library Preparation Kit (Diagenode ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples in lysis buffer were boiled for 10 minutes and sonicated (Bioruptor, Diagenode) for 15 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated using the BioruptorR Sonication device (Diagenode) six times at 30/60 cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... Extracted chromatin was sheared with the Bioruptor sonicator (Diagenode) using 20 cycles of 30 s on ...
-
bioRxiv - Immunology 2023Quote: ... lysed using a Bioruptor sonication device (Diagenode) (settings ...
-
bioRxiv - Genomics 2023Quote: ... and sonicated for 6 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode). Insoluble material was removed by centrifugation at >16,000g for 10 min at 4°C ...
-
bioRxiv - Systems Biology 2023Quote: ... the samples were sonicated in a non-contacting BioRuptor Pico system (Diagenode) for 30 cycles at 4 °C (30 s-on and 30 s-off ...
-
bioRxiv - Cell Biology 2023Quote: ... and the Bioruptor Pico Sonicator (Diagenode; Cat #B01060010) for 7 min ...
-
bioRxiv - Neuroscience 2023Quote: ... then sonicated for 10 cycles of 30 s each on/off in a Bioruptor plus (Diagenode) in presence of 50 mg Protein Extraction beads (C20000021 ...
-
bioRxiv - Molecular Biology 2023Quote: ... sonicated with a Bioruptor Pico (Diagenode) and immunoprecipitated with anti-Myc (c-Myc/N-Myc (D3N8F ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were sonicated (5x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
-
bioRxiv - Molecular Biology 2023Quote: ... and H3pan (Diagenode C15410324) as loading control ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... or H3K9me3 (Diagenode C15410056). Antibodies were then pulled down with the A/G beads and washed thoroughly ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then incubated for 14 hours with the following antibodies: H3K27ac (Diagenode, C15410196), H3K27me3 (abcam ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... H3K4me3 (Diagenode, C15410003), or H3K9me3 (Diagenode C15410056) ...
-
bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
-
bioRxiv - Genetics 2023Quote: ... H3K27ac antibody (Diagenode) and isotype control IgG (provided in the kit ...
-
bioRxiv - Genetics 2023Quote: ... the cross-linked chromatin was immunoprecipitated with ∼1μg of CTCF antibody (Diagenode), H3K4me1 antibody (Diagenode) ...
-
bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sonication was performed on a Bioruptor (Diagenode) on high power ...
-
bioRxiv - Cancer Biology 2023Quote: ... nuclei were extracted from 50,000 cells and the nuclear pellet was resuspended in the Tn5 transposase reaction mix (Diagenode, Cat# C01070012-30). The transposition reaction was incubated at 37°C for 30 min ...
-
bioRxiv - Plant Biology 2023Quote: ... Chromatin was fragmented by a Bioruptor (Diagenode) programed at high intensity for 3 x 7.5 min (cycles of 30 s on and 30 s off ...
-
bioRxiv - Plant Biology 2023Quote: ... The isolated input DNA was fragmented by using a Bioruptor® Plus sonication device (Diagenode) to obtain the desired DNA fragment size (enriched at 200 bp).
-
bioRxiv - Plant Biology 2023Quote: ... Protease Inhibitor Cocktail) and sonicated by using a Bioruptor® Plus sonication device (Diagenode) to obtain the desired DNA fragment size (enriched at 500 bp) ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
-
bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
-
bioRxiv - Plant Biology 2023Quote: ... The chromatin solution was sonicated using a Biorupter Plus (Diagenode) with 10 cycles of 30 sec pulse/90 sec cooling at high power at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3 µg of either IgG control antibody (Diagenode; C15410206) or of a specific antibody were applied ...
-
bioRxiv - Molecular Biology 2023Quote: ... anti-H3K3me2 (Diagenode; C15410035), and anti-H3K4me3 (Diagenode ...
-
bioRxiv - Molecular Biology 2023Quote: ... and anti-H3K4me3 (Diagenode; C15410003).
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... the Microplex library preparation kit v2 (Diagenode, C05010012) was used for indexed sequencing library preparation with chromatin immunoprecipitated DNA ...
-
bioRxiv - Molecular Biology 2023Quote: Chromatin-immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the One-day ChIP kit protocol (Diagenode; C01010080). Beads were exchanged to Dynabeads™ Protein A (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... and lysed by sonication (Bioruptor, Diagenode, high intensity for 10 minutes with an ON/OFF cycle of 30 seconds ...
-
bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043) ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.7ug Tn5 (Diagenode C01070010) previously loaded with nextera adapters loaded ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tissues were homogenized using a Bioruptor standard water bath sonicator (Diagenode, UCD-200) with the following settings ...
-
bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043), 0,1% Tween-20 ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by water bath sonication (Diagenode Bioruptor Plus) to reduce sample viscosity and a 5-minute incubation at 95°C to ensure complete protein denaturation ...
-
bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...