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Citations for Stemcell Technologies :
7851 - 7900 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and then an Easy Sep PE sorting kit (#18554; Stem Cell Technologies, Vancouver, BC) was used to perform the positive selection of CD63+ EVs in the ultrafiltration (110 kDa exclusion filter ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Single cell suspensions were then cryopreserved using CryoStor CS10 solution (STEMCELL Technologies) and stored in liquid nitrogen.
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bioRxiv - Genomics 2020Quote: ... prefilled with 15 mL Lymphoprep™(Stemcell technologies, # 07851), followed by a centrifugation step of 30 min at 400g (acceleration 5 ...
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bioRxiv - Molecular Biology 2020Quote: ... in mTeSR media (StemCell Technologies) and passaged using versene once reaching 70% confluency and plated at 1:6 density.
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bioRxiv - Molecular Biology 2020Quote: ... in mTeSR media (StemCell Technologies). Cells were passaged using versene once reaching 70% confluency and plated at 1:6 density ...
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bioRxiv - Neuroscience 2020Quote: ... iPSC were grown to near confluence in E8 media before detaching colonies in gentle dissociation buffer (StemCell Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Cancer Biology 2020Quote: ... BTICs were cultured in serum-free medium (SFM) (NeuroCult proliferation medium; STEMCELL Technologies) supplemented with 2 µg/ml heparin sulfate (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... Neutrophils were enriched using a negative selection neutrophil isolation kit (Cat #19666, 18000, Stemcell Technologies, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 2 µg/ml heparin sulfate (STEMCELL Technologies) or SFM supplemented with 20 ng/ml human recombinant epidermal growth factor (hEGF ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and VEGF (Stem Cell Technologies) at 50ng/mL each ...
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bioRxiv - Cell Biology 2020Quote: ... To begin the protocol cells were washed twice and incubated in phase I medium comprised of APELII medium (Stem Cell Technologies) supplemented with BMP-4 (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: The ALDH activity of adherent cells was measured using the ALDEFLUOR kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: BM MNCs cells were seeded on 35-mm culture dishes in triplicate in methylcellulose medium supplemented with cytokines (MethoCult GF M3434, STEMCELL Technologies). CFU-Cs (colony forming units in culture ...
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bioRxiv - Bioengineering 2020Quote: Primary human bronchial epithelial cells (HBECs) were isolated immediately from bronchial brushing into T25 flasks containing Pneumacult™ Ex-Plus Basal Media (Stemcell Technologies, Vancouver Canada) with Pneumacult™ Ex-Plus 50x Supplement ...
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2020Quote: from CD4-T cells following the protocol described in the EasySep PE-selection kit (STEMCELL technologies). Cells from the negative fraction (CD4+CD25- ...
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bioRxiv - Immunology 2020Quote: Mouse bone marrow dendritic cells (DC) were generated as previously described.32 CD11c+ DC were purified by magnetic separation (EasySep Positive Selection; Stemcell Technologies, Cambridge, MA). Mature-DC were induced by adding LPS (200 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Cell Biology 2020Quote: ... control-MPC and Givi-MPC were dissociated using Accutase (Stem Cell Technologies) and resuspended in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs were grown and maintained on vitronectin coated six-well plate in mTeSR1 medium (Stem Cell Technologies) with daily change.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Human iPSCs were originally cultured in mTesR medium (STEMCELL Technologies, Cambridge, MA) and more recently in StemFlex medium (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were passaged at 80% confluence using Gentle Cell Dissociation Reagent (STEMCELL Technologies) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Both cell lines were routinely cultured on six-well plates coated with growth factor reduced Matrigel in mTeSR media (STEMCELL Technologies) with daily media replacement ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids were collected from Matrigel by Dispase (Stemcell Technologies, MA), resuspended in HistoGel (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Neuroscience 2020Quote: ... Rosettes were selected at 14 DIV by Rosette Selection Reagent (StemCell Technologies) and patterned to forebrain NPCs with EB media containing 20ng/ml FGF2 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... in mTeSR1 (StemCell Technologies) supplemented with 10 ng/ml FGF2 StemBeads (StemCultures ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Evolutionary Biology 2020Quote: ... the cell suspension was frozen in CryoStor medium (STEMCELL Technologies, Cat. No. ST07930) with Mr ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides are then stained for 1-2 days in Carmine Alum (Stem Cell Technologies; Catalog No. 07070) at RT ...
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bioRxiv - Immunology 2020Quote: ... Monocytes and neutrophils were harvested from the PBMC and PMN fractions using immunomagnetic negative and positive selection respectively and the protocols were performed according to the manufacturer’s recommendation (Stemcell Technologies, Cambridge, UK).
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bioRxiv - Neuroscience 2020Quote: ... in mTESR1 (Stem Cell Technologies) culture media ...
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bioRxiv - Neuroscience 2020Quote: ... Aggrewells coated with anti-adherence rinsing solution (StemCell Tech), U bottom non-treated polystyrene plates (ThermoFisher #168136 ...
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bioRxiv - Neuroscience 2020Quote: ... Media was changed daily and cells were split at a 1:6 ratio using Collagenase IV (Stemcell Technologies, #07909) every 5-6 days.
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bioRxiv - Developmental Biology 2020Quote: ... HiPSCs were adapted and maintained with mTeSR™1 culture medium (05850, Stemcell Technologies) on Corning® Matrigel® Basement Membrane Matrix ...
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bioRxiv - Genetics 2020Quote: ... The hPSCs were regularly passaged by treating with Dispase (Stem Cell Technologies).
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bioRxiv - Genetics 2020Quote: ... hPSCs were maintained in Matrigel (Corning)-coated plate in mTeSR1 medium (Stem Cell Technologies) in a 37 °C humidified 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... HSPCs were purified using a CD34+ selection kit (Stem Cell Technologies, Canada); the purity of CD34+ cells was 90%–99% ...
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bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
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bioRxiv - Immunology 2020Quote: ... whole blood was purchased from StemCell Technologies.
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bioRxiv - Immunology 2020Quote: ... and 25 ng/ml IL-10 (STEMCELL Technologies, Vancouver, Canada). After stimulation ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Remaining whole blood was diluted 1:1 with 1% RPMI (Table S4) and layered into a SEPMATE tube (STEMCELL Technologies, Cat#85450) pre-loaded with lymphoprep (Alere Technologies ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...