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Citations for Stemcell Technologies :
7751 - 7800 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: PBMCs of anonymous healthy donors were isolated from buffy coats purchased from the Beijing Red Cross Blood Center using density gradient cell separation by Ficoll (Lymphoprep™, STEMCELL Technologies) following the protocol approved by the Institutional Review Board of School of Medicine ...
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bioRxiv - Immunology 2020Quote: ... The cells were grown under submerged monolayer conditions as above in a two-day feeding cycle with PnemaCult™ Ex-Plus media supplemented according to manufacturer’s recommendations (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Pathology 2020Quote: ... WiCell) and WTC11c hiPSCs (a gift of Dr. Bruce Conklin, Gladstone Institutes, San Francisco) were maintained in mTeSR1 (Stemcell Technologies) on tissue culture dishes coated with Matrigel (Corning ...
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bioRxiv - Immunology 2020Quote: ... Primary human airway epithelial cells isolated via bronchial brushings from consented healthy individuals were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then incubated in a digestion buffer of 5 Units/mL dispase (Stemcell Technologies, #07913) and 0.1 mg/mL DNase I (Worthington ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X collagenase (300 Units/mL) / hyaluronidase (100 Units/mL) (Stemcell Technologies, #07912), 2% FBS (Corning ...
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bioRxiv - Immunology 2020Quote: ... in MammoCult media (StemCell Technologies, Vancouver, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells were extracted from blood drawn from healthy donors using density gradient separation (Lymphoprep, Stem Cell Technologies, Vancouver, BC, Canada) and cultured at 1 × 106 cells/ml in RPMI complete medium containing 1μg/ml Concanavalin A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
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bioRxiv - Cell Biology 2020Quote: ... Erythroblasts were cultured in a serum-free mononuclear cell (MNC) medium containing the following cytokines diluted in Stem Span Serum Free Expansion Medium (Stem Cell Technologies, CA): 40□ng/mL insulin-like growth factor 1 (IGF1) ...
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bioRxiv - Bioengineering 2020Quote: ... was based on StemSpan SFEM I (Stem Cell Technologies) supplemented with hSCF (50 ng/mL ...
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bioRxiv - Bioengineering 2020Quote: Human hematopoietic progenitors were cultured in StemSpan SFEM I (Stem Cell Technologies) supplemented with human stem cell factor (hSCF ...
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bioRxiv - Immunology 2020Quote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... Mononuclear cells were isolated over a Ficoll gradient (Lymphoprep: Stem Cell Technologies, 7851) prior to separation into CD34+ and flowthrough (CD34− ...
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bioRxiv - Developmental Biology 2020Quote: ... with 1:800 dilution of membrane permeant NeuroFluor™NeuO probe (cat# 01801, StemCell technologies) to detect neurons and 1:500 dilution of cell permeable DNA dye Vybrant® DyeCycle™ Ruby Stain (cat# V10309 ...
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bioRxiv - Genomics 2020Quote: ... Neutrophils were freshly isolated directly from whole blood by negative selection immunomagnetic purification with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Genomics 2020Quote: ... Monocytes were isolated from PBMCs by positive selection (STEMCELL Technologies; cat. no. 17858), to ensure inclusion of the CD14+/CD16+ fraction ...
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Genomics 2020Quote: ... followed by immunomagnetic enrichment for the specific cell subset with EasySep Human cell enrichment kits (STEMCELL Technologies). B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies ...
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bioRxiv - Genomics 2020Quote: ... Mononuclear cell subsets were obtained by isolation of PBMCs by gradient centrifugation in SepMate tubes (STEMCELL Technologies; cat. no. 85460), with Ficoll-Paque PLUS (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/mL recombinant human IL-15 (StemCell Technologies, Cat # 78031). Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: Primary human NK cells were enriched from the peripheral blood of human donors using RosetteSep Human NK cell Enrichment Cocktail (Stem Cell Technologies, Cat #15065) and cultured overnight in RPMI-1640 (Corning ...
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bioRxiv - Genomics 2020Quote: ... cells were counted using an automated cell counter and 900,000 iPSCs were re-suspended in 15ml of mTeSR medium (Stemcell Technologies 85850) with 50μM ROCK inhibitor (Santa Cruz sc-216067A) ...
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bioRxiv - Immunology 2020Quote: ... cells in full media were incubated with Human Recombinant IFNα-2A (10ng/mL, StemCell Technologies 78076.1) or IFNγ (10ng/mL ...
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bioRxiv - Immunology 2020Quote: ... or T cells (EasySep Human T cell Isolation Kit, Stemcell Technologies, #17951). After isolation ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Immunology 2020Quote: ... counted and treated with 10 U ml−1 DNAse I (Stemcell Technologies, #) for 15 min at RT in order to prevent cell clumping ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMC via negative selection (EasySep Cell Separation; StemCell Technologies), added to a mix of irradiated feeders consisting of freshly isolated allogeneic PBMC combined with log-phase-growth RPMI 8866 cells (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... or after NK cell enrichment using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies). To measure antigen-specific NKCL responses ...
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bioRxiv - Immunology 2020Quote: ... after CD3+ T cell depletion using the EasySep Human CD3 Positive Selection Kit II and the and the protocol for Using EasySep Positive Selection Kits for Cell Depletion provided by the manufacturer (STEMCELL Technologies) or after NK cell enrichment using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were collected from whole blood samples of healthy donors using SepMate™ tubes (STEMCELL Technologies, Canada) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and Ter119) followed by magnetic separation using EasySep Positive Selection kit (Stem Cell Technologies). After enrichment ...
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bioRxiv - Cell Biology 2020Quote: ... coated plates in mTeSR1 medium (StemCell Technologies, 05850) at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: CD34+ cells were cultured overnight in SFEMII media (Stemcell Technologies #09605) supplemented with Pen-Strep (50 Units/mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... and cultured overnight in SFEMII media (StemCell Technologies #09605) supplemented with Pen-Strep (50 Units/mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with StemSpan Myeloid Expansion supplement (SCF, TPO, G-CSF, GM-CSF) (Stemcell Technologies) and 1% penicillin/streptomycin in a humidified 5% CO2 atmosphere at 37 °C.
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were grown in serum-free StemSpan SFEMII (Stemcell Technologies, Vancouver, Canada) supplemented with StemSpan Myeloid Expansion supplement (SCF ...
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bioRxiv - Cancer Biology 2020Quote: ... Colony Assay for human CD34+ HSPCs and AML patient cells were performed by plating 10000 cells and 3000 cells respectively on methylcellulose (MethoCult #H4434, Stem Cell Technologies). CFU-GM (Granulocyte/Macrophage) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with 2ml of 0.25% Trypsin/EDTA solution (Stem Cell Technologies, cat #07901) and 2ml of 5mg/mL dispase (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Fourth and fifth mammary gland pairs were resected from mice and were enzymatically digested with collagenase/hyaluronidase (Stem Cell Technologies, cat #07919) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... differentiation was started (day 0) by changing to STEMdiff APEL medium (STEMCELL Technologies) supplemented with 8 μM CHIR99021 (R&D Systems) ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Cell Biology 2020Quote: RMG1 cells were cultivated in RPMI-1640 medium (Stemcell technologies) supplemented with 10% fetal bovine serum (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Developmental Biology 2020Quote: ... Organoids were singularized using Accutase (STEMCELL Technologies) and washed with cold PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... and reseeding in mTeSR™-1 medium (STEMCELL Technologies) supplemented with Y-276932 (10μM ...
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bioRxiv - Developmental Biology 2020Quote: Unencapsulated 50 cell aggregates were created by dissociation of human iPSCs with Accutase (STEM CELL Technologies) and reseeding in mTeSR™-1 medium (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Encapsulated aggregates were allowed to recover for 24 hours in mTeSR™-1 medium (STEMCELL Technologies) in rotary suspension ...
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bioRxiv - Developmental Biology 2020Quote: ... and once with mTeSR™-1 medium (STEMCELL Technologies). Encapsulated aggregates were allowed to recover for 24 hours in mTeSR™-1 medium (STEMCELL Technologies ...