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Citations for Stemcell Technologies :
7901 - 7950 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Monocytes were enriched from the same leukapheresis products using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were isolated directly from blood using the CD8+ T Cell Enrichment Cocktail (StemCell Technologies) and density gradient centrifugation according to the kit’s instructions ...
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bioRxiv - Immunology 2020Quote: ... using memory CD8+ isolation kit (StemCell Technologies). T cells were harvested and washed three times with Opti-MEM (LifeTechnologies) ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...
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bioRxiv - Immunology 2020Quote: ... in mTeSR™1 (STEMCELL Technologies) medium ...
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bioRxiv - Immunology 2020Quote: ... iPSCs were harvested using Gentle Cell Dissociation Reagent (STEMCELL Technologies). Viable cells were counted and seeded at a density of 5 × 104 cells per cm2 on Matrigel® in mTeSR™1 containing 10 μM Y-27632 ROCK pathway inhibitor (Selleckchem) ...
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bioRxiv - Immunology 2020Quote: ... the medium was changed to STEMdiff™ Mesoderm Induction Medium (STEMCELL Technologies, defined, xeno-free medium) and the medium was replaced daily for the next consecutive 4 days ...
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bioRxiv - Genetics 2020Quote: ... 10 μM Y-27632 (StemCell Technologies, UK) and 5 μM DAPT (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... Y-27632 (StemCell Technologies, UK), A83-01 (TGF-β inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumors (6–8 mm in diameter) were mechanically dissociated and digested with collagenase/hyaluronidase (#07912, STEMCELL Technologies) containing DNase I (4 U/ml ...
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bioRxiv - Cell Biology 2020Quote: ... mESCs were passaged using Accutase (STEMCELL Technologies 07920) and centrifuged at 244 x g for 5 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... were maintained on Matrigel (Corning)-coated surfaces in mTeSR1 (STEMCELL Technologies) as previously decribed46 ...
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bioRxiv - Bioengineering 2020Quote: ... CD34+ EC progenitors were purified with an EasySep Magnet kit (STEMCELL Technologies) with a CD34 antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... For passaging the cells Accutase (StemCell Technologies, Vancouver, BC) was used for 5 min at 37C and then stopped and pelleted by gentle centrifugation with iPSC media containing 10μM Y-27632 (Selleckchem ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% CloneR (Stem Cell Technologies Cat. 05888) into one well of a Geltrex (Thermo Fisher Cat ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with mTeSR1 medium (Stemcell Technologies) in a 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... WA01 cells were passaged using Versene solution (Stemcell Technologies) every 6-8 days.
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bioRxiv - Bioengineering 2020Quote: Cerebral organoids were fabricated using a stemdiff cerebral organoid kit (Stemcell Technologies). Initially ...
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bioRxiv - Bioengineering 2020Quote: ... we then topped the organoids with 1 mL of fresh BrainPhys neuronal medium (Stemcell Technologies) and incubated them in a 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... on Vitronectin XF(tm) (Stemcell technologies) coated (1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... http://www.hipsci.org) were maintained in TeSR(tm)-E8(tm) (Stemcell Technologies) on Synthemax® II-SC Substrate (10µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... and passaged mechanically using PBS-EDTA or Gentle Cell Dissociation Reagent (Stemcell Technologies). The human immortalised brown adipocyte cell line Paz6 was cultured as described previously 12 ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Genetics 2020Quote: ... CD34+ mobilized peripheral blood HSPCs were thawed and cultured in SFEM containing CC110 supplement (Stemcell Technologies 02697) for 2 days ...
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bioRxiv - Genetics 2020Quote: ... CD34+ mobilized peripheral blood HSPCs were thawed and cultured in SFEM containing CC110 supplement (Stemcell Technologies 02697) for 2 days for thaw recovery ...
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bioRxiv - Genetics 2020Quote: ... HUDEP-2 cells were cultured in a base medium of SFEM (Stemcell Technologies 9650) containing to a final concentration of dexamethasone 1uM (Sigma D4902-100MG) ...
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bioRxiv - Cancer Biology 2020Quote: ... Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies, Vancouver, British Columbia, Canada). After 14 days ...
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bioRxiv - Genetics 2020Quote: ... dissociated with Accutase (Stem Cell Technologies, 7920), and added to fresh medium on a new plate and/or added to freezing medium for storage.
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bioRxiv - Neuroscience 2020Quote: ... Human iPSCs were maintained in complete mTeSR1 medium (STEMCELL Technologies) on Matrigel (Corning ...
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bioRxiv - Immunology 2020Quote: ... Mononuclear cells were recovered with lymphocyte separation media and sepMate tubes (Stemcell Technology, Vancouver, Canada) by centrifugation ...
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bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
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bioRxiv - Genetics 2020Quote: ... cell lines were cryopreserved in CryoStor® (07930, StemCell Technologies) for further use ...
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bioRxiv - Genetics 2020Quote: ... Expansion phase medium for HUDEP-2 cells consists of SFEM (Stemcell Technologies #09650) base medium supplemented with 50 ng/ml recombinant human SCF (R&D systems #255-SC) ...
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bioRxiv - Cancer Biology 2020Quote: ... in SepMate-50 tubes (Stemcell Technologies, 85450) and subsequently ACK-lysed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following previously described directions for EB generation in Aggrewell plates (StemCell Technologies Document #DX21397), 6 × 105 cells in 1.5 ml media were plated per well ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PBMCs were isolated using Lymphoprep (Stem Cell Technologies, 07801), followed by expansion for 9 days in Expansion Media composed of Stemspan H3000 (Stem Cell Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by expansion for 9 days in Expansion Media composed of Stemspan H3000 (Stem Cell Technologies, 09850) supplemented with 50μg/ml ascorbic acid (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The timing of the protocol (StemCell Technologies Document #DX21849) was followed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Experiments on ZEB2+/− organoids were generated according to the STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570) or alternatively with fibrous microscaffolds and media formulation as previously described (Lancaster et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570) was used for organoid culture ...
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bioRxiv - Evolutionary Biology 2020Quote: Cerebral organoids with telencephalic identity from human and ape cells were generated as previously (Lancaster et al. 2017) with the following modification: Aggrewell 800 plates (StemCell Technologies) containing 300 microwells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and were held in place by a drop of Matrigel that was allowed to polymerize before addition of expansion media (StemCell Technologies, 08570). Time-lapse movies were acquired on an Andor Revolution Spinning Disk microscope in an incubation chamber set to 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with CD34+ Expansion Supplement (StemCell Technologies, 02691). Bone marrow (BM ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with erythroid Expansion Supplement (StemCell Technologies, 02692). Erythroid differentiation medium was changed every 2 days and differentiated erythroblasts were collected for qPCR or HPLC analysis from day7 to day14.
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bioRxiv - Cell Biology 2020Quote: ... and cultured in SFEM II medium (StemCell Technologies, 09605) supplemented with CD34+ Expansion Supplement (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to SFEMII medium (StemCell Technologies, 09605) supplemented with erythroid Expansion Supplement (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: Cord blood (CB) CD34+ cells were purified with Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, 17896) and cultured in SFEM II medium (StemCell Technologies ...