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Citations for Stemcell Technologies :
7801 - 7850 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged by dissociation with Accutase (STEM CELL Technologies) and re-seeded in mTeSR™-1 medium supplemented with the small molecule Rho-associated coiled-coil kinase (ROCK ...
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bioRxiv - Developmental Biology 2020Quote: ... and fed daily with mTeSR™-1 medium (STEMCELL Technologies) (32) ...
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bioRxiv - Genetics 2020Quote: ... These were cultured in PneumaCult (#05009; StemCell Technologies) with 2 mM L-glutamine (25030-024 ...
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bioRxiv - Developmental Biology 2020Quote: ... spheroids were transferred to the MEA plate containing BrainPhys media (STEMCELL Technologies). For acute experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Pathology 2020Quote: ... YM1/ECF-L (Stem Cell Technologies; 01404, 1:100) and PCNA (abcam ab92552 - 1:200) ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Synthetic Biology 2020Quote: ... and PBMCs were isolated using Lymphoprep (STEMCELL Technologies, 07851). Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... and passed weekly at a ratio of 1:10-50 dependent on colony confluency using ReLeSR (StemCell Technologies).
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bioRxiv - Neuroscience 2020Quote: ... in mTeSR1 (StemCell Technologies). Validation was performed through ...
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bioRxiv - Neuroscience 2020Quote: ... and heparin (2 μg/mL, StemCell Technologies, #07980).
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bioRxiv - Neuroscience 2020Quote: ... with NeuroCult Proliferation Supplement (StemCell Technologies, #05701), EGF and bFGF (10 ng/mL each ...
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bioRxiv - Neuroscience 2020Quote: ... the resulting single cell suspension was cultured in NeuroCult Basal Medium (StemCell Technologies, #05701) with NeuroCult Proliferation Supplement (StemCell Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... culture media was changed to TeSR-E7 (StemCell Technologies). Defined iPSC-like colonies were manually isolated 3-4 weeks post-transfection and expanded on Matrigel (Corning ...
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bioRxiv - Cancer Biology 2020Quote: Colony forming cell (CFC) assay for murine cells was performed by plating 1000 cells on methylcellulose (MethoCult #M3434, Stem Cell Technologies). Colony Assay for human CD34+ HSPCs and AML patient cells were performed by plating 10000 cells and 3000 cells respectively on methylcellulose (MethoCult #H4434 ...
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bioRxiv - Microbiology 2020Quote: ... maintained in feeder-free conditions in a mTeSR™1 (85850; Stem Cell Technologies) medium supplemented with 1% Penicillin-Streptomycin (P0781 ...
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bioRxiv - Systems Biology 2020Quote: Monocytes were enriched using RosetteSep human monocyte enrichment cocktail (Stem Cell Technologies) and purified by density gradient centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... incomplete methylcellulose medium M3431 (Stem Cell Technologies, Vancouver, Canada) was supplemented with 30% TCM obtained from 5×106 total tumor cells after they were adjusted to culture for 24 h in serum free medium ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... thawed organoids were rinsed with 2% fetal bovine serum (FBS, from STEMCELL Technologies) in Hank’s Balanced Salt Solution (HF) ...
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bioRxiv - Genetics 2020Quote: ... 400 μl of IntestiCult™ Organoid Growth Medium (STEMCELL Technologies) was added to each well ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Mouse Neutrophil Enrichment Kit was used as per the included protocol (StemCell Technologies, cat. 19762). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole murine blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Direct Human Neutrophil Isolation Kit was used as per standard protocol (StemCell Technologies, cat. 19666). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Cell Biology 2020Quote: ... Jurkat cells (Clone E6-1, ATCC® TIB-152(tm)) and PBMCs (Stemcell technologies, Catalog # 70025.1) were cultured at 0.5–1.5 × 10^6 cells/ml in RPMI 1640 media (Corning ...
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bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with Proliferation supplement (Stem Cell Technologies #05751) and growth factors ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/mL Heparin (Stem Cell Technologies #07980) and L-glutamine (2mM ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with Proliferation supplement (Stem Cell Technologies #05701), 20 ng/mL recombinant human EGF (Gibco #PHG0313) ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Mouse NeuroCult basal medium (Stem Cell Technologies #05700) without growth factors ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human cord blood CD34+ cells were purchased from STEMCELL Technologies GmbH (Cologne ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids were sheared with 1000 p pipette tips or incubated with Accutase (Stem cell technology) at 37 °C for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPSCs were dissociated into single cells using Accutase (Stem Cell Technologies) at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Immunology 2020Quote: ... FcγRII or FcγRIII block was performed by addition of receptor specific mAbs (Stem cell technologies, IV.3 and 3G8) at a 1:100 dilution at the beginning of the incubation period ...
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bioRxiv - Immunology 2020Quote: ... Primary NK cells were separated from donor PBMCs via magnetic bead negative selection (Stem Cell technologies). 96well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and VEGFA were obtained from Stem Cell technologies. Recombinant CD20 was obtained as a peptide (aa141-188 ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: Human CB CD34+ cells were purchased from STEMCELL Technologies GmbH (Cologne ...
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bioRxiv - Immunology 2020Quote: ... monocytes were isolated from PBMCs of Malian donors by a negative selection monocyte isolation kit (Stem Cell Technologies) without depleting CD16 such that monocytes were isolated ‘untouched’ ...
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bioRxiv - Immunology 2020Quote: ... Elutriated monocytes were further purified with a monocyte isolation kit (Stem Cell technologies) to minimize donor-to-donor variability in contaminating cell populations ...
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bioRxiv - Immunology 2020Quote: ... and enriched for CD8+ cells with an EasySep™ negatively selective magnetic isolation kit (STEMCELL Technologies). OT-I cells prepared for use in nuclei isolation experiments were then treated with CellTrace™ Violet reactive dye (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... cells were dissociated with Accutase (STEMCELL Technologies, #07920), and the suspension was seeded onto glass coverslips coated with 20 µg/cm2 poly-L-ornithine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... cells at week 3 of terminal differentiation were switched to BrainPhys medium (STEMCELL Technologies), and experiments were conducted after a total of 5–6 weeks of differentiation ...
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bioRxiv - Neuroscience 2020Quote: ... and cultured using mTeSR1 (STEMCELL Technologies, #05850) changed daily ...