Labshake search
Citations for Stemcell Technologies :
8101 - 8150 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... At DIV3 the media was replaced by a BrainPhys based media (Stemcell technology) supplemented with 2 % B27 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were directly isolated from buffy coats using negative magnetic selection according to manufacturer’s instructions (Stem Cell Technologies). NK cells were then stained using the following antibodies ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) and plasma were isolated using Lymphoprep (Stemcell Technologies, Vancouver, BC, Canada), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Immunology 2020Quote: ... Two types of controls were used in all experiments except when noted: activated cells co-cultured in the presence of CD3/CD28/CD2 activating bead (STEMCELL Technologies, Vancouver, Canada) and non-activated cells co-cultured in the presence of complete media without beads ...
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bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2020Quote: ... were isolated from perioperative human patients’ brain tissues (Nervous System Tumor Bank) using EasySep Human CD8 T Cells Isolation Kit (STEMCELL Technologies) following the tissue digestion as previously described [68 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction remaining after the positive selection of CD14+ cells was used for pan-DC enrichment employing the EasySep human pan-DC enrichment kit (StemCell Technologies). Total pan-DC were resuspended with 20,000 CD14+ cells and sent for sequencing ...
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bioRxiv - Immunology 2020Quote: ... following the manufacturer’s instructions (Stemcell Technologies). Preparations were checked by May-Grünwald-Giemsa stain of smears of samples ...
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bioRxiv - Immunology 2020Quote: ... following the manufacturer’s instructions (Stemcell Technologies, Vancouver, Canada). Lymphocytes were separated by RosetteSep Human T cell Enrichment and by RosetteSep Human B cell Enrichment cocktail ...
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bioRxiv - Immunology 2020Quote: ... using the EasySep Human Naive B Cell Enrichment Kit (19254; STEMCELL Technologies), from healthy donor PBMCs ...
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bioRxiv - Microbiology 2020Quote: ... SepMate tubes (Stemcell Technologies) and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Bioengineering 2020Quote: Hydrophobic TLR7 adjuvant (R848) was purchased from STEMCell Technologies (Vancouver, CA). The RIG-I adjuvant PUUC (MW = 60,200 g/mole ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: Swine PBMCs were obtained from swine whole blood through density gradient centrifugation using Sepmate15 (Stem cell technologies) and lymphocyte separation media (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... and were maintained at ALI for 21+ days in a Pneumacult™ ALI growth medium (Stem Cell Technologies) at 37°C/5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2020Quote: ... harvested from healthy individuals were expanded using Pneumacult™-Ex Plus (Stem Cell Technologies) and seeded on 24 well hanging inserts ...
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bioRxiv - Microbiology 2020Quote: ... coated 6-well plates in mTeSR1 media (Stem Cell Technologies) at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were passaged with ReleSR (STEMCELL Technologies) when cells reach approximately 80% confluence ...
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bioRxiv - Microbiology 2020Quote: hESCs were maintained in mTeSRTM1 medium (STEMCELL Technologies, Vancouver, Canada) as we previously described with daily medium replenishment ...
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bioRxiv - Microbiology 2020Quote: The hESCs were differentiated into definitive endoderm using the commercial STEMdiff Definitive Endoderm kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ectoderm differentiation was performed with basal Medium (DMEM/F12, 10% knockout serum replacement (KOSR) and 1% Glutamax) supplemented with 10 μM SB431542 (STEMCELL Technologies) and 200 nM Noggin (Peprotech ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Microbiology 2020Quote: Whole blood samples from scrub typhus patients were collected and peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep™ density gradient medium and SepMate™ tubes (Stemcell Technologies, Vancouver, Canada). The isolated PBMC were aliquoted and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2020Quote: ... larvae were tricaine-anesthetized and embedded within optically clear glass imaging cubes (Mizar Imaging) in embryo medium with 1% low melt agarose and 20% OptiPrep (Stemcell Technologies, Inc. ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... cells were cultured in Mesencult proliferation medium (Mesencult Basal Medium supplemented with MSC stimulatory supplements (StemCell Technologies, Grenoble, France) and 2% antibiotics and glutamine) ...
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bioRxiv - Immunology 2020Quote: Mouse Ly6Chi monocytes were purified from the bone marrow of naïve mice (C57BL/6J or CD45.1) following the protocol according to the manufacturer (Stemcell Technologies, 19861). Mice were intranasally vaccinated with CDG (5µg ...
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bioRxiv - Immunology 2020Quote: ... Splenic cells were enriched for NK cells as described in the EasySep Positive Selection Kit (STEMCELL Technologies) manufacturer’s suggested protocol by selecting with an anti-“lineage” cocktail (anti-CD3,-CD4,-CD8,-CD19,-Gr1 ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen cord blood and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technologies, Vancouver, Canada), frozen Plerixafor- and/or G-CSF-mobilized peripheral blood of patients with SCD ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Molecular Biology 2020Quote: ... and rabbit anti-GFAP (1:500; Stem Cell Technologies 60128) were diluted in 1% blocking solution (normal donkey serum in 0.3% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... was purchased from Stemcell technologies.
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bioRxiv - Immunology 2020Quote: ... B cells were purified magnetically (STEMCELL Technologies) and stained with anti-CD19 ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from the US were collected between 2016 - 2018 and purchased from Stemcell Technologies, Canada ...
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bioRxiv - Immunology 2020Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
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bioRxiv - Immunology 2020Quote: ... The PBMCs were isolated via density gradient centrifugation (Lymphoprep, STEMCELL Technologies, Canada). Isolated PBMC were cryopreserved and stored in liquid nitrogen until used in the assays.
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bioRxiv - Immunology 2020Quote: Monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer’s instructions (STEMCELL Technologies, Canada). Monocytes were cultured in the DC medium for 6 days ...
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bioRxiv - Immunology 2020Quote: ... Purity and viability of isolated T cells was assessed using flow cytometry prior to cryopreservation of isolated T cells in CryoStore CS10 cryopreservation media (Stem Cell Technologies) at a density of 1-1.5×107 cells per ml.
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bioRxiv - Immunology 2020Quote: ... PBMCs were separated from whole blood by density gradient centrifugation technique using Lymphoprep media (cat # 07851, STEMCELL technologies, Cambridge, MA, USA). PBMCs from CHOP patients were either frozen or used to isolate B cells immediately ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacture’s instruction (17864, STEMCELL Technologies, Cambridge, MA, USA). CD19+CD138+ plasma cells were isolated from PBMCs by positive selection using biotin conjugated CD138 antibody (352322 ...
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bioRxiv - Immunology 2020Quote: ... and Easysep release human biotin positive selection cocktail (17653, STEMCELL Technologies). CD19+CD11c+ B cells (ABCs ...
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bioRxiv - Immunology 2020Quote: Sorted LSK cells from BM of control Rag 1−/− mice were suspended in methylcellulose medium and co-cultured with sorted OCLs from BM of colitic or control Rag 1−/− mice and with or without control mesenchymal stromal cells (MSCs) to quantify clonogenic colony forming units in triplicate cultures (MethoCult GF M3434, Stem Cell Technologies). Colonies were scored after 10–14 days ...
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bioRxiv - Immunology 2020Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Total CD3+ T cells were isolated from unfractionated PBMCs using the EasySep™ Human T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag™-2 magnet (ThermoFisher Scientific ...