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Citations for Stemcell Technologies :
7701 - 7750 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: Cardiomyocyte differentiation was induced using the STEMdiff Cardiomyocyte Differentiation and Maintenance kit (STEMCELL Technologies) in the CTST platform ...
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bioRxiv - Bioengineering 2020Quote: Endoderm differentiation was induced using the TeSR-E8 optimized STEMdiff Definitive Endoderm Kit (STEMCELL Technologies) in the CTST platform ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Bioengineering 2020Quote: ... LM1s was dissociated using Accutase (STEMCELL Technologies). Planarian cells were collected by mechanical dissociation of whole animals using a razorblade and pipetting in 1X PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Cancer Biology 2020Quote: ... freshly prepared 0.48 μg/ml hydrocortisone (Stem Cell Technologies), 2 mM L-glutamine (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were then washed twice in Ca2+ and Mg2+ free PBS (Stem Cell Technologies, Vancouver, Canada). The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Developmental Biology 2020Quote: ... Primary cells were resuspended in Epicult-B (Stemcell Technologies) media containing 2% Matrigel and 12,000 cells were seeded into each chamber in a total volume of 400 μL ...
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bioRxiv - Cancer Biology 2020Quote: ALDH activity was measured by flow cytometry with ALDEFLUOR (StemCell Technologies, Vancouver, BC, Canada) as recommended by the manufacturer ...
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bioRxiv - Immunology 2020Quote: Naïve CD4+ T cells were negatively isolated from fresh PBMCs by EasySep (Stem Cell Technologies; >95% purity). Cells were stimulated (Ellis et al. ...
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bioRxiv - Genomics 2020Quote: ... iPSCs were adapted from DEF-CS culturing system to mTeSR™1 (STEMcell Technologies) culturing system by seeding 40K/cm2 on Geltrex (ThermoFisher ...
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bioRxiv - Bioengineering 2020Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies).
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bioRxiv - Biophysics 2020Quote: ... Patient-derived GBM cells were plated in NeuroCult NS-A serum-free medium (StemCell Technologies, Vancouver, BC, Canada) supplemented with 20 ng/ml of epidermal- and 10 ng/ml of fibroblast-growth factor (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 1% Heparin Solution (STEMCELL Technologies, 07980), 2% Amphotericin B (Fungizone ...
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bioRxiv - Cancer Biology 2020Quote: ... Adam Green (University of Colorado) and grown in NeuroCult NS-A media (Stemcell Technologies) supplemented with penicillin-streptomycin (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: The ALDH activity of was measured using Aldefluor kit (Stem Cell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... in mTSER1 medium (Stem Cell Technologies) and amplified using EDTA (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... PSC were thawed in presence of Y-27632 (10µM, Stemgent or Stem Cell Technologies) and the culture media was changed every day.
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bioRxiv - Developmental Biology 2020Quote: ... with Y-27632 (10µM, Stemgent or Stem Cell Technologies), CHIR-99021 (3 µM or 4 µM Selleckchem ...
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bioRxiv - Genetics 2020Quote: ... (#05871/05852, StemCell Technologies Inc.) and were routinely passaged with collagenase (#07909 ...
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bioRxiv - Genetics 2020Quote: ... and were routinely passaged with collagenase (#07909, StemCell Technologies Inc.). Cells were routinely tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit from Lonza (LT07-118) ...
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bioRxiv - Genetics 2020Quote: ... Neural induction of hES H1 cells expressing different shRNA constructs was performed using STEMdiff™ Neural Induction Medium (#05831, StemCell Technologies Inc.) in combination with a monolayer culture method according to the manufacturer’s technical bulletin (#28044 ...
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bioRxiv - Developmental Biology 2020Quote: ... iCRISPR cells were cultivated using standard feeder-free conditions in mTeSR1 (StemCell Technologies) on matrigel-coated plates ...
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bioRxiv - Developmental Biology 2020Quote: ... iCRISPR cells were cultivated using standard feeder-free conditions in mTeSR1 (StemCell Technologies) on matrigel-coated plates ...
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bioRxiv - Immunology 2020Quote: The indicated number of FACS-purified cells were seeded into methylcellulose culture medium (M3534, StemCell Technologies), plated in in 35 mm dishes and incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... by density gradient centrifugation using Lymphoprep (Stem cell technologies, #07801). CD8 T cells were purified from the PBMC fraction using CD8 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils were isolated using the EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies, #19762), as per the manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... in complete mTeSR™1 (Stem Cell Technologies) supplemented with 100 units/mL of penicillin-streptomycin (Gibco™ ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation PBS was aspirated and the cell pellet was tapped loose and resuspended in mTeSR™-1 spin-EB medium consisting of mTeSR™-1 (Stem Cell Technologies), supplemented with 1 mM Rock-inhibitor (Y27632 ...
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bioRxiv - Cell Biology 2020Quote: ... For AggreWells™800 (Stemcell Technologies, 300 micro-wells), plates were first prepared After preparing the plate ...
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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bioRxiv - Genetics 2020Quote: ... 10 μM Y-27632 (StemCell Technologies, UK) was added to the medium during the proliferation stage to promote basal cell proliferation.
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bioRxiv - Cell Biology 2020Quote: ... Megakaryocyte potential was assessed using the MegaCult culture system (StemCell Technologies) in accordance with manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with Dispase (Stem Cell Technologies) and the epidermis was removed with gentle scraping using a No.15-blade scalpel ...
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bioRxiv - Genomics 2020Quote: ... All-Trans Retinoic Acid 0.1 μM (RA, STEMCELL Technologies, Cat.: #72262), and Purmorphamine 0.5 μM (PMN ...
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bioRxiv - Genomics 2020Quote: ... Cells were passaged every four to six days as clumps using ReLeSR™ an enzyme-free reagent for dissociation (StemCell Technologies, Cat.: #05872) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s recommendations in complete mTeSR™-Plus™ Medium (StemCell Technologies, Cat.: #05825). The culture medium was replaced daily and confirmed mycoplasma free ...
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bioRxiv - Pathology 2020Quote: ... human iPS-GFP IMR-90-4 reporter cells were maintained in an undifferentiated state in mTesr1 media on plates coated with Matrigel and were routinely passaged with Versene (StemCell Technologies, Vancouver, CA) in a ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pre-coated with collagen solution (StemCell Technologies, Cologne, Germany). Cells were maintained with 200 µL growth medium in the apical and 600 µl of growth medium in the basolateral compartment ...
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bioRxiv - Systems Biology 2020Quote: ... To determine intracellular ALDH activity the Aldefluor® Kit (Stem Cell Technologies) was used according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... as per manufacturer’s instructions (STEMCELL Technologies, Vancouver, Canada). Successful transition of iPSCs into NPCs was determined using immunofluorescence for the absence of the stem-cell marker OCT4 and presence of the NPC-marker PAX6 (Figure 1A) ...