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Citations for Macherey-Nagel GmbH :
3151 - 3200 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... purified with Nucleospin Gel and PCR Clean-up (Macherey-Nagel, Germany), and cloned into pEX-A (Eurofins Genomics ...
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bioRxiv - Neuroscience 2019Quote: ... see Addgene #98034 for reference sequence) virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
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bioRxiv - Cancer Biology 2019Quote: Cells pellet (1.5*10^6) were collected at different time points after LPS stimulation and DNA was extracted with the Nucleospin tissue kit (Macherey-Nagel, 740952). The genomic DNA was eluted in 50 μl of BE buffer (5 mM Tris/HCl ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from Ca9-22 and HSC3 cells using NucleoSpin (Macherey-Nagel, Düren, Germany). Northern blot analysis was conducted using DIG Easy Hyb and an RNA probe made with Dig RNA labeling Mixture (Roche Diagnostics ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA was isolated using the Nucleospin RNA (Macherey-Nagel) and first-strand cDNA was then transcribed using the SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... and from the 2.0 μm glassfiber filters using NucleoSpin Plant II Midi kit (Macherey-Nagel, Düren, Germany), following the manufacturers protocol except that Qiagen buffers were used instead of those supplied with the kit ...
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bioRxiv - Genetics 2019Quote: ... Endotoxin-free plasmid DNA from sequence-verified clones was isolated using the Nucleobond Xtra Midi EF Kit (Macherey-Nagel, Düren Germany), ammonium acetate precipitated and re-suspended in MaxCyte buffer (MaxCyte Inc. ...
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bioRxiv - Physiology 2019Quote: ... checked with test strips for rapid detection of glucose (Medi-Test, Macherey-Nagel, Düren, Germany), respectively ...
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bioRxiv - Physiology 2019Quote: ... and then purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel Milan, Italy). For each target gene ...
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bioRxiv - Physiology 2019Quote: ... the plasmid was purified using the NucleoSpin® Plasmid kit (Macherey-Nagel, Milan, Italy) from 4mL of culture ...
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bioRxiv - Physiology 2019Quote: ... purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Italy) and subsequently sequenced.
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bioRxiv - Plant Biology 2019Quote: ... Total DNA was extracted using the NucleoSpin plant kit (Macherey-Nagel, Düren, Germany) or the DNeasy 96 Plant Kit (QIAGEN ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Molecular Biology 2019Quote: ... the supernatant was resolved on PEI-TLC (Macherey-Nagel) in 0.5 KH2PO4 buffer (pH 3.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s instructions (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Standard PCR reactions were performed using KAPATaq DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Genomics 2019Quote: ... PCR products were purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel #740609) and analyzed with capillary electrophoresis using an Agilent 2100 Bio-analyzer and an Agilent DNA 1000 kit (#5067-1504).
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bioRxiv - Microbiology 2019Quote: ... following incubation with 20 mg/ml lysozyme in P1 buffer (NucleoSpin® Plasmid Kit, Macherey-Nagel) overnight at 30 °C ...
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bioRxiv - Cell Biology 2019Quote: ... NucleoSpin Blood L kit (#740569.10) was from Macherey-Nagel. ECL Western Blotting Detection Reagent (#RPN2106 ...
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bioRxiv - Molecular Biology 2019Quote: ... Viral RNA was extracted from the mosquito pools using MN Nucleo Spin® RNA (Macherey-Nagel GmbH & Co. KG, ref. 740955.250) and cDNA was immediately synthetized using Hi-capacity RNA-to-DNA™ kit (Applied Biosystems™ ...
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bioRxiv - Immunology 2019Quote: ... purified (NuceloSpin Gel & PCR Clean-up Kit; Macherey-Nagel), and amplicons cloned into pCR4-TOPO (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: ... plasmid DNA was prepared using the NucleoBond Xtra Midi Kit (MACHEREY-NAGEL, Germany) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2019Quote: ... RNA extraction was performed using a modified protocol of the NucleoSpin 96 RNA tissue kit (Macherey-Nagel Gmbh&Co. KG, Düren, Germany) and using a vacuum system [12] ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Germany). 1 µg of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2019Quote: Total RNA was isolated using NucleoSpin® (Macherey-Nagel) RNA isolation kit ...
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bioRxiv - Genetics 2019Quote: ... Cells were then treated with JQ1 at the effective IC50 value for 6 hours and RNA was harvested using NucleoSpin RNA Plus columns (Macherey-Nagel). RNA expression levels were quantified after reverse transcription with MMLV H-(Promega ...
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bioRxiv - Epidemiology 2019Quote: ... using the DNA extraction kit NucleoSpin® Tissue (Macherey-Nagel, Germany), and following the standard protocol for human or animal tissue and cultured cells ...
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bioRxiv - Immunology 2019Quote: Total cellular RNA was isolated by the NucleoSpin® RNA II kit (Macherey-Nagel), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... The strain was grown overnight in de Man-Rogosa-Sharpe (MRS) medium (Carl Roth, Karlsruhe, Germany) and DNA was extracted using the NucleoSpin 96 tissue kit (Macherey-Nagel, Düren, Germany), with an extra cell lysis step using 20 mg/mL of lysozyme (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were retrotranscribed (High-Capacity cDNA Reverse Transcription kit ...
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bioRxiv - Biochemistry 2019Quote: ... followed by gel extraction and clean-up using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The products were mixed at an equimolar ratio and sent for paired-end sequening on Illumina HiSeq2000 to Novogene Bioinformatics Technology Co. ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... Starting from overnight grown liquid cultures of respective pAGM9121 constructs (prepared as described in section 5) plasmid mixtures were prepared using a NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, DE). Following the preparation ...
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bioRxiv - Genetics 2019Quote: ... and NucleoSpin RNA (Macherey-Nagel, Duren, Germany). All samples were sequenced on the Illumina HiSeq 2500 platform as single-end 50-bp reads and mapped to the human genome (UCSC build hg19) ...
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bioRxiv - Cell Biology 2019Quote: mRNA was isolated using the NucleoSpin® RNA/Protein kit (Macherey-Nagel). Purified mRNA was transcribed into cDNA with the QuantiTect®Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... total RNA was extracted using Nucleozol (Macherey-Nagel, 740404.200). Enrichment of polyadenylated RNA from total RNA was performed using Oligo(dT ...
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bioRxiv - Molecular Biology 2019Quote: ... tRNAs were further purified using the NucleoBond RNA/DNA 400 kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA from iPSCs was isolated using a commercial kit (MACHEREY-NAGEL) as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... as described by the manufacturer (Macherey-Nagel). Fractions were collected in 0.5 ml volume and electrophorized on 12% sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Size selection on each pool was performed via gel extractions using NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL) targeting fragment sizes comprised between 250 and 500 bp ...
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bioRxiv - Neuroscience 2019Quote: ... a Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) was used and the instructions were followed as provided by the company ...
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bioRxiv - Genomics 2019Quote: ... while total RNA was extracted using the Nucleospin RNA plant kit with the protocol modified for maximum yield (Macherey-Nagel, Oensingen, Switzerland). RNA concentration was determined with a Qubit 3.0 (Thermofisher).
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bioRxiv - Biochemistry 2019Quote: ... Glycopeptides were purified using a solid phase extraction on Chromabond C-18 sorbent (Macherey-Nagel, Düren, Germany). Samples were loaded onto beads in 0.1% TFA ...
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bioRxiv - Plant Biology 2019Quote: Total RNA from 4 week old Arabidopsis WT Col-0 and mtamutants28 was extracted as described above followed by polyA enrichment using NucleoTrap® mRNA isolation kit (Macherey-Nagel). Immunoprecipitation was performed with EpiMark N6-Methyladenosine Enrichment Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... The cssDNA was cleaned by a NucleoSpin® (Macherey-Nagel GmbH & Co. KG, Düren, Germany) column ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were purified using NucleoSpin Plasmid (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... We extracted soil and root samples with the NucleoSpin Soil DNA extraction kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions (Knauth et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...