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Citations for Macherey-Nagel GmbH :
3101 - 3150 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Microbiology 2019Quote: ... using the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel, Germany). For these previous steps ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using a Millipore NucleoFast 96 PCR kit following the manufacturer’s recommendations (Macherey-Nagel, Düren, Germany) and sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Nitrate and nitrite concentrations were qualitatively monitored using test stripes (Quantofix nitrate/nitrite, Macherey-Nagel, Germany). A correlation between cellular dry weight (CDW ...
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bioRxiv - Microbiology 2019Quote: ... and the resulting PCR products were cleaned using a NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel, Düren, Germany). Samples were prepared for sequencing using a BigDye Terminator 3.1 kit (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: Plasmid DNA was purified using the Nucleospin Plasmid kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kits (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... purification of the high molecular weight product by gel extraction kit using PCR Clean-up kit (Macherey-Nagel), and subsequent ligation using T4 DNA ligase.
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bioRxiv - Microbiology 2019Quote: Total DNA was extracted manually using Macherey-Nagel extraction kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 48 h later RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel). RNA levels were determined with Power SYBR® Green RNA-to-CT™ 1-Step Kit using a StepOne Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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Molecular profiling of driver events and tumor-infiltrating lymphocytes in metastatic uveal melanomabioRxiv - Cancer Biology 2019Quote: RNA was extracted from the indicated cell lines with Nucleospin RNA II kit (Macherey-Nagel), and converted to cDNA using iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty microliters have been collected for protein analysis and RNA was extracted from remaining supernatant with the Nucleospin RNA extraction II Kit (Macherey-Nagel).
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bioRxiv - Plant Biology 2019Quote: RNA was extracted using NucleoSpin® RNA Plant Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...
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bioRxiv - Synthetic Biology 2019Quote: ... digested with KpnI and purified again with PCR Clean-up kit (Macherey-Nagel, Germany). The p416-loxP-KmR-TEFmut6-yECitrine plasmid was linearized with KpnI and dephosphorylated with Antarctic Phosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Total DNA of the pellet was extracted by kit (Macherey-Nagel,GmßH&Co.KG,Germany) and concentration was measured (NanoDrop ND-1000) ...
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bioRxiv - Immunology 2019Quote: ... RNA from sorted MAIT cells was isolated using the Nucleospin RNA isolation kit (Macherey-Nagel, Düren, Germany) as per the manufacturer’s instructions and stored at −80 °C ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was prepared using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then subjected to solid-phase extraction (SPE) in 96-well plates cation exchange columns (Macherey-Nagel HR-XC), pre-conditioned with 500 μl methanol and equilibrated with 500 μl milliQ H2O ...
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bioRxiv - Developmental Biology 2019Quote: ... genomic DNA was isolated from a confluent 3.5- or 10-cm dish with the Nucleospin Tissue Kit (Macherey-Nagel). The rearranged region between the promoter and GOIs (500-600 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
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bioRxiv - Molecular Biology 2019Quote: ... The plasmids were purified using the NucleoBond® XtraMidi kit (Macherey-Nagel) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2019Quote: ... purified using a PCR purification kit (Macherey-Nagel Nucleospin; 740609), and sent for Illumina sequencing ...
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bioRxiv - Genomics 2019Quote: ... and muscle using Trizol and the Nucleospin® RNA II kit (MACHEREY-NAGEL), following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... the extracted ChIP DNA was further re-purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). The region-specific primer sets used for the ChIP-qPCR analysis have been mentioned in Table S2.
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bioRxiv - Molecular Biology 2019Quote: ... the Nucleospin RNA II kit (Macherey-Nagel, Duren, Germany) was used for total RNA extraction following the manufacturer’s instruction (Gondard et al. ...
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bioRxiv - Microbiology 2019Quote: ... RNA and DNA were extracted simultaneously using the Nucleospin RNA kit (Macherey-Nagel) plus the NucleoSpin RNA/DNA Buffer Set (Macherey-Nagel ...
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bioRxiv - Microbiology 2019Quote: ... plus the NucleoSpin RNA/DNA Buffer Set (Macherey-Nagel) procedures ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA was extracted from a single adult female using the NucleoSpin Tissue XS (Macherey-Nagel) according to manufacturer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Further purification of the extracted DNA was done with NucleoSpin® gDNA Clean-up (Macherey-Nagel). For laboratory bred F2 offspring of the two-generation acclimation experiment ...
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bioRxiv - Microbiology 2019Quote: ... difficile strains was done using standard methods.33 Routine DNA extractions were performed using the Nucleospin Plasmid Easypure (Macherey-Nagel) and DNeasy Blood and Tissue (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... according to the manufacturer‟s instructions (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Standard PCR reactions were performed using KAPATaq DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and on tick organs from pre-fed field-collected females using the Nucleo Spin RNA (Macherey-Nagel) kit according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and products of the expected size were cut out and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). 10 ng of purified PCR product was then used as template for RNA probe synthesis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... probes were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany), re-suspended in 50 µL of DEPC water ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and purified using the NucleoSpin RNA II Kit (Macherey-Nagel, Duren, Germany).
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bioRxiv - Evolutionary Biology 2019Quote: DNA from 30 males and 30 females from the fish production unit of the fishing federation of Ille-et-Vilaine (Pisciculture du Boulet, Feins, France) was extracted with a NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The cooled samples were filled up with deionized water to a volume of 25 ml and filtered through ashless filter paper (MN640 m, Ø125 mm, manufactured by Macherey-Nagel, Düren, Germany). Absorption was measured at 213.8 nm for Zn and 248.3 nm for Fe with an atomic absorption spectrometer (model 11003 ...
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bioRxiv - Molecular Biology 2019Quote: Cells were lysed for RNA 36h-40h post-transfection with 350µL RNA lysis buffer LBP (Macherey-Nagel), and RNA were extracted with the NucleoSpin RNA Plus kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNA were extracted with the NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... compounds were separated by reverse-phase chromatography using Ultra Performance Liquid chromatography (UPLC IClass, Waters) using a Nucleodur C18ec column (2.0 mm × 150 mm, 5 μm particle size, Macherey-Nagel). Elution was achieved by increasing the acetonitrile/water (milliQ filtrated on 0.22 μm ...
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bioRxiv - Cancer Biology 2019Quote: ... guide RNAs and Cas9 were expressed from plasmids that were purified with the NucleoBond® Xtra Midi Kit (Macherey-Nagel). In the remaining mixes ...
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bioRxiv - Genetics 2019Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into pGEM®Teasy vector (Promega) ...
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bioRxiv - Plant Biology 2019Quote: ... RNA was extracted from frozen samples using the NucleoSpin RNA Plant kit (Cat# 740949, Macherey-Nagel, Germany) following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2019Quote: ... Frozen samples were subjected to RNA extraction using the NucleoSpin RNA Plant kit (Cat# 740949, Macherey-Nagel, Germany) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... The DNA was purified with NucleoSpin PCR clean-up columns (Macherey-Nagel) using NTB buffer (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2019Quote: ... using NTB buffer (Macherey-Nagel) and PCR amplified as for ChIP ...
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bioRxiv - Bioengineering 2019Quote: ... filtered over a 0.45 μm filter (type PA-45/25, Macherey-Nagel, Germany), and diluted with Milli-Q water to reach the desired concentration range for quantification between 1 and 100 mg L−1 ...
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bioRxiv - Neuroscience 2019Quote: RNA viral genomes were extracted from two Tripodi lab (EnvA/ SiR-CRE and EnvA/Sir-FLPo) and one Wickersham lab (RVΔG-4mCherry) rabies virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...