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Citations for Bio-Rad :
3601 - 3650 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was estimated colorimetrically using a Bio-Rad protein assay and 25 μg of protein with equal volume of 2X Laemmli sample buffer (Bio-Rad) was denatured at 70°C for 10 min ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: Samples from m7GTP pull-down assays and lysates were boiled at 70°C for 10 min in Laemmli sample buffer (Bio-Rad) and resolved in 10% or 4-15% gradient SDS-PAGE gels ...
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bioRxiv - Immunology 2022Quote: ... the beads were re-suspended in assay buffer and the plates read on a Bio-Plex 3D Suspension Array System (Bio-Rad). Antigens and controls included BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... The total content of glycogen was normalized to content of soluble protein measured using the Bradford assay (Bio-Rad 500–0006) with BSA standard curves.
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the level of cytokines in mouse serum, were assessed by using customized plates (Luminex assay, R&D) and analyzed on a Bio-Plex 200 instrument (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Neuroscience 2022Quote: ... culture supernatants were collected and cytokine levels in the samples were measured using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... conditioned culture medium was collected after 24 h and assessed by Luminex assay (Bio-techne) and a Bioplex-200 machine (Bio-Rad). Bioplex manager 6.1 software was used to determine analyte concentrations.
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bioRxiv - Neuroscience 2022Quote: The entire volume of each flow through obtained from the pull-down assay was separated on Mini-PROTEAN TGX gels (Bio-Rad) and proteins transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2022Quote: ... Cytokines concentrations in BALF were assessed using a Bio-plex magnetic bead assay (Luminex, Biotechne, France) and analyzed using a Bio-plex 200 workstation (Biorad, France).
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used the multiplex bead array system Bio-Plex Pro Mouse Chemokine Assay Panel 31-Plex (#12009159, Bio-Rad Laboratories GmbH), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... IL-28A/IFN-λ2 and IL-29/IFN-λ1) using a Bio-Plex ProTM Human Inflammation Panel 1 Express assay (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR assays were conducted using gene specific primers (Supplementary table X. Primer list) using SYBR green master mix (Bio-Rad). The reaction was run for a total of 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... The copy number of stx2 in each cellular DNA sample was determined by droplet digital PCR using the EvaGreen assay (Bio-Rad) with the stx2-specific primers described above ...
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bioRxiv - Biochemistry 2024Quote: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was then centrifuged at 14,000rpm for 5 minutes and the Ire1-LD concentrations in supernatant and pellet (after washing three times in HMK buffer) were measured using the Bradford protein assay (Bio-Rad) with bovine serum albumin as a standard ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was used to quantify the total protein concentration in the sample by using the DC protein assay (Bio-Rad) and a BSA standard curve [54] ...
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bioRxiv - Immunology 2022Quote: ... the beads were re-suspended in assay buffer and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein amount in each lysate sample was measured using a DC™ protein assay (Bio-Rad, Cat#5000113, #5000114, #5000115). Caspase-3 activity in each sample was calculated as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of extracts (quantified using Lowry assay) were subjected to SDS-PAGE analysis on 4-15% Criterion TX gels (Bio-Rad), transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Two microliters of each RNA sample were amplified in duplicate assays with a CFX detection system (Bio-Rad, Hercules, CA, USA) using TaqMan RT-PCR master mix (Quanta Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The post-nuclear fraction was collected and protein concentration was determined by the Bradford protein assay (Bio-Rad Laboratories, Hercules, USA) using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... The qRT-PCR assay was performed using ChamQ SYBR Master Mix (Vazyme, Q311-02) on Bio-rad CFX96 real-time PCR system (Biorad, USA). All primers used in the present study were listed as following ...
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bioRxiv - Cell Biology 2023Quote: ... gene expression of tdTomato [TaqMan probe FAM - Mr07319439_mr (Thermofisher)] and GAPDH (Rufino-ramos et al. 2022)(Al Ali et al. 2021) was analyzed using ddPCR following the PrimePCR ddPCR Gene Expression Probe Assay (Bio-Rad). Using the manufacturer’s protocol droplets were generated with DG8 Cartridge using a QX200 droplet generator and PCR was performed with thermal cycling conditions using QX200 Droplet Reader and QuantaSoft Software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Microbiology 2023Quote: Cytokine and chemokine levels were measured in mouse serum using the Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad). Cytokines and chemokines were recorded on a MAGPIX machine (Luminex ...
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bioRxiv - Immunology 2023Quote: ... the supernatant containing extracted soluble protein was collected and protein concentration was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (5000006, Bio-Rad) according to the standard protocol provided by the manufacturer.
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bioRxiv - Immunology 2023Quote: ... PrimePCRTM PCR Primers assay plates were custom made with validated primers for use with EvaGreen dye-based chemistry (Bio-RAD, USA). Samples were run on CFX96 Touch Real-Time Detection System (Bio-RAD ...
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bioRxiv - Physiology 2023Quote: ... The resulting lysates were used for quantification of the total protein content with the DC protein assay (Bio-Rad, Hercules, USA) according to Lowry et al ...
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bioRxiv - Microbiology 2023Quote: ... 15 µg of membrane and soluble fraction were loaded after normalization by total protein quantification using the Bio-Rad Protein Assay (Bio-Rad) onto a 12% acrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Bioengineering 2023Quote: ... followed by an additional wash and resuspension in Assay Buffer for readout by high-throughput cytometry (Bio-Rad ZE5 cell analyzer). Results were decoded using Nomic software ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR assay was carried out using gene-specific fluorescently labelled probes and using CFX96 PCR system by Bio-Rad. The nucleotide sequences used for real-time qPCR analysis are showed in Table 1 ...
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bioRxiv - Bioengineering 2023Quote: ... Assay temperature kinetics study was performed in PCR tubes at 20 μL volume with the same assay concentrations as discussed above in a CFX Connect Real-Time System (Bio-Rad).