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Citations for Bio-Rad :
3801 - 3850 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay, Bio-Rad, Richmond, CA) (Lowry et al ...
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bioRxiv - Cell Biology 2020Quote: ... Equal total protein amounts for each sample were used in co-immunoprecipitation assays and analyzed by SDS-PAGE on Criterion gels (Bio-Rad, Hercules, CA). Co-immunoprecipitation assays were performed as described (49 ...
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bioRxiv - Neuroscience 2021Quote: To determine HTT protein expression in fibroblasts and iNs protein concentration was determined using Bradford Protein Assay (Bio-Rad Laboratories, Mississauga, ON, Canada). HTT immunoblotting was performed as previously described (83) ...
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bioRxiv - Microbiology 2020Quote: The optimal annealing temperature for duplex ddPCR assay with RNR and ORF1 primers was determined by a thermal gradient in the S1000™Thermal cycler (Bio-Rad, USA). The temperature range were 53 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fish gill DNA was isolated using the conventional phenol-chloroform method [31,32] and 200 ng of each DNA sample was subjected to qPCR assays using the CFX Connect™ Real-time System (Bio-Rad, USA). The resulting Cq value was used to compute bacterial DNA in the fish gills using the equation ...
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bioRxiv - Cell Biology 2021Quote: ... Droplet digital PCR (ddPCR) primer/probe assays for copy number variation (CNV) were obtained from Bio-Rad (mouse ND1/HEX, mouse ActB/FAM). The ddPCR mix was as follows ...
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were prepared in Laemmli SDS-sample buffer supplemented with Protease Inhibitor Cocktail (Nacalai Tesque, Kyoto, Japan) and protein concentrations were quantified by the DC protein assay (Bio-Rad, CA, USA). Protein concentrations were normalized if necessary and boiled for 5 min with 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred from a 10% SDS-PAGE gel onto a nitrocellulose membrane for a Western blot assay using the Trans-Blot turbo transfer system (Bio-Rad, Hercules CA). Phosphorylated SakA (P- SakA ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Protein lysates were quantified using BCA assay (Pierce, Waltham, MA) and equivalent amounts were subjected to SDS-PAGE on a 4-15% TGX polyacrylamide gel (Bio-Rad, Hercules, CA). Proteins were transferred onto a polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... primers and assays were performed on the Bio-Rad CFX Connect Real-Time PCR system using SsoAdvanced™ Universal SYBR® Green Supermix (BioRad, 1725272). The β-Actin housekeeping gene was used to normalize the variability in expression level ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μl of each sample was taken out separately for measuring protein concentration using Bradford protein assay reagent (BIO-RAD, Hercules, CA, USA). 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Synthetic Biology 2019Quote: ... Roughly 5 ng of genomic DNA was mixed with primers and probes for a human RPP30 copy number assay labeled with HEX (Bio-Rad, Hercules, CA) as an internal control ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was measured from the 50 μL sample of homogenate (described above) using a Quick Start Bradford assay (BioRad, Cat. Number 5000205). Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2019Quote: Protein isolated from placental homogenates were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Hercules, CA, USA) and the Luminex system (Bio-Rad ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein pellet was resuspended in 100 mM ammonium bicarbonate (AMBIC) with 20% methanol and quantified by the Lowry method (Bio-Rad DC assay). A total of 100 µg of protein was reduced by adding tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined by BCA assay as directed by the manufacturer (Pierce, P123227) and samples were prepared with Laemmli sample buffer (Bio-Rad, 161-0747), 100mM DTT (Sigma ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Molecular Biology 2021Quote: ... the supernatants were transferred to new tubes and protein concentrations were determined using the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Amylose resin samples were transferred to columns and protein-bound beads were washed with lysis buffer until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Maltose-binding protein fusions were eluted with 12 ml elution buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Cell Biology 2022Quote: 100 microlitres of the secreted fractions and 100 µl of 1:20 diluted (in PBS) lysates from the secretion assays were loaded into a bio-blot microfiltration blotting system (BioRad. Cat. Nº: 1706545). Samples were left to flow through a 0.45 µm nitrocellulose blotting membrane (Amersham Protran ...
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bioRxiv - Neuroscience 2022Quote: ... The clear supernatants were then transferred into clean tubes and total protein concentration measured using a DC protein assay (Bio-Rad, Hercules, CA). To prepare for ELISA ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed using RIPA buffer and total protein concentration was quantified using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006). Twenty micrograms of protein were incubated in Laemmli sample buffer at 95°C for 5 min and subjected to electrophoresis on a 3-15% gradient polyacrylamide gel ...
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bioRxiv - Microbiology 2022Quote: ... We quantified cytokine concentrations with the Bio-Plex Pro Mouse Cytokine 23-plex Assay and the Bio-Plex 200 system (Bio-Rad Laboratories, Inc.), or with VeriKine Mouse Interferon Alpha or Beta ELISA kits (PBL Assay Science ...
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bioRxiv - Microbiology 2024Quote: ... One hundred nanograms of gDNA from each tissue type or isolate were used to perform ddPCR assay using a QX200 Droplet Digital PCR SystemTM (Bio-Rad, Hercules, USA), as described by von Ammon et al ...
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bioRxiv - Molecular Biology 2024Quote: ... STAT3 thermal shift assays (TSA) were performed in a 96-well PCR plate (Hard-shell and thin wall PCR plates, Bio-Rad, Hercules, California). Temperature gradient from 25 to 95 °C (increasing ramp rate of 0.05 °C/sec ...
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bioRxiv - Cancer Biology 2024Quote: ... Serum hormones were quantified on a Luminex MAGPIX platform using the Bio-Plex Pro Mouse Diabetes 8-Plex and Adiponectin Assays (Bio-Rad, Hercules, CA) and the IGF-1 mouse Luminex Discovery Assay (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... of the IDH1 R132H mutation were determined in 69 tumor sections from case one and the patient’s blood using the PrimePCR IDH1 R132H mutant assay and the QX100 Droplet Digital PCR system (Bio-Rad Inc., Hercules, CA). An initial serial dilution of a positive control was performed to optimize the input concentration of genomic DNA from each section and to assess the reliability of the assay ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria-containing pellet was resuspended in THE buffer without BSA and the protein concentration measured using the Bradford Protein Assay (Bio-Rad, Munich, Germany). The protein concentration was adjusted to 10 µg/µl with THE buffer.
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... DELTAgene Assay primers were purchased from Fluidigm and amplicon DNA was quantified using SsoFast™ EvaGreen Supermix with Low ROX (Bio-Rad Laboratories). Gene expression data were normalized based on the values for GAPDH mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Three ddPCR reactions were set up for each of the mRNA fusion assays with the following volumes: 10 µL 2x ddPCR Supermix for Probes (no dUTP) (PN 186-3023, Bio-Rad, Hercules, CA), 1 µL 20x mRNA Fusions Assay ...
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bioRxiv - Pathology 2023Quote: ... The total protein concentrations of each liver samples were determine using a standard curve generated with BSA at different known concentration using the Quick Start™ Bradford Protein Assay (Bio-Rad, U.S.A.). On the basis of the measure protein cencetrions ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Immunology 2023Quote: Extracted whole-kidney protein was diluted in PBS to 900 µg/mL and subjected to a Bio-Plex Mouse Cytokine 23-plex Assay (#M60009RDPD, Bio-Rad, Hercules, CA), according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and protein-bound beads were washed with 60ml lysis buffer until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50 mM Tris-HCl 7.4 ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Immunology 2024Quote: Circulating plasma levels of cytokines and chemokines in PTB patients were measured using the Luminex MAGPIX Multiplex Assay system (Bio-Rad, Hercules, CA). Luminex Human Magnetic Assay Kit 45 Plex (R&D Systems ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were cleared by centrifugation at 10,000 g for 5 minutes and protein concentration was determined using the DC Protein Assay (Bio-Rad Laboratories, Watford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the reverse primer and the probe sequences were designed using Primer3 following Bio-Rad guidelines for the primer/probe assay design (BioRad Bulletin 6407, n.d.). The designed primers and probe (Table 1 ...
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bioRxiv - Plant Biology 2019Quote: ... An iScript™ cDNA Synthesis Kit (Bio-Rad) was used to synthesize cDNA ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using RNAeasy kit (Bio-Rad) and used to perform RNA-seq or to perform qPCR as previously described (Khan et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... using an iScript cDNA synthesis kit (Bio-Rad). The cDNA was then subjected to qRT-PCR using the Fast SYBR Green Master Mix (Applied Biosystems/Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... 7D using the iScript cDNA synthesis kit (BioRad), from 10,000-40,000 cells ...
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bioRxiv - Genetics 2019Quote: ... and iScript cDNA synthesis kit (Bio-Rad, USA) was used to reverse transcribe 1-2 µg of RNA per sample ...