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Citations for Bio-Rad :
3551 - 3600 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins were then dialyzed against Lysis Buffer to remove GSH and quantified using a Bradford assay per the manufacturer instructions (BioRad, 5000006) prior to being stored at −80oC ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM β-mercaptoethanol) until the washes indicate a protein concentration of less than 0.1 mg/mL by Bradford assay (Bio-rad). The protein was eluted with elution buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... cAMP content in TCA extracts was determined by radioimmunoassay (RIA) (83) and normalized to protein [(determined using a dyebinding protein assay (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... ddPCR was performed according to manufacturer’s instructions for the 2019-nCoV CDC ddPCR Triplex Probe Assay (Bio-Rad, Hercules, California, USA). PCR reaction mixtures were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred into a new tube and and the protein concentration was determined using a Bradford assay (Biorad #5000006) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were transferred to a new tube and an aliquot were used for protein quantification using the Bradford assay (Bio-Rad). A total of 10-20 μg of protein lysates were separated on 8% gels by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: Equilibrium target binding assays were performed using a double-membrane filter-binding setup with a slot blot vacuum filtration device (BioRad SF) containing an upper nitrocellulose membrane (to capture protein and protein/RNA complexes ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR assay and data analysis were performed using a CFX96 Touch Real-Time PCR Detection System (Biorad, Hercules, CA, USA). The amplification and quantification conditions used were an initial denaturation step of 3 min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... with target specific primers and the assay ran on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). A 139-bp fragment was amplified for FBN11228 (F-CCAGCATCGGTACAACGGAA and R-AAGCTCGTGTTTTCGTGCTG) ...
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bioRxiv - Bioengineering 2022Quote: ... All the eluted fractions were tested using a Bradford reagent assay (diluted 1:5 with ddH2O) (190 μL Bradford, 10 μL sample) (Bio-rad). Positive fractions were desalted using 100 kDa amicons (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the EV was determined using the Quick Start™ Bradford protein assay according to manufacturer’s guidelines (BioRad, UK). In short ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of SsoAdvanced Universal SYBR Green Supermix and 1 μl of the following DNA templates (Prime PCR Assay, Bio-Rad): mt-Co2 (mitochondrial ...
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bioRxiv - Microbiology 2021Quote: ... Images of whole plate phage assay were captured using a ChemiDoc XRS+ imaging system using the epi-light filter (BioRad, USA).
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bioRxiv - Plant Biology 2021Quote: ... The qPCR assay was performed by using UltraSYBR Mixture (KWBIO, #CW2602) on the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad). ACTIN was used as a normalization control and the relative expression of each test gene was calculated by the 2-ΔΔCT method (Livak & Schmittgen ...
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Fifty microliters of homogenate were used to measure cytokine and chemokine protein abundance using a Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... Concentration of the VLPs was estimated by Bradford assay and densitometry using SDS PAGE analysis of serially diluted VLP preparations on Mini-PROTEAN Precast Gels (Bio-Rad), stained with GelCode Blue Stain (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Half of the produced supernatant (see above) was dosed by the Bradford method (Protein Assay Dye Reagent Concentrate, Biorad #500-0006) and digested with 1% trypsin (weight/weight ...
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bioRxiv - Genomics 2019Quote: ... For KRAS (G13D) and AKT1 (E17K) mutant and wild type PrimePCR™ ddPCR™ Mutation Assays were obtained from Bio-Rad (dHsaCP2000013 and dHsaCP2000014 ...
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bioRxiv - Immunology 2019Quote: ... Samples were then centrifuged for 30 min and protein concentration was measured using the DC Protein Assay according to standardized protocols (Bio-Rad). Protein concentration in supernatants was normalized to 1 mg/mL and frozen at −80°C until assay ...
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bioRxiv - Physiology 2019Quote: ... The mitochondrial pellet was resuspended in 25μL of isolation buffer and protein was quantitated using the Bradford assay (BioRad, #500-0006).
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bioRxiv - Cancer Biology 2019Quote: ... Copy number assay was performed using 3 ng gDNA as a template and commercially available probes for MET (dHSACP2500321, FAM, Bio-Rad) and NSUN3 (dHSACP2506682 ...
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bioRxiv - Microbiology 2019Quote: ... Lungs were homogenized in D-PBS containing protease inhibitors to perform the cytokines quantification using a commercial multiplex mouse cytokine bead assay (Bio-Rad, Bio-Plex Pro Mouse Cytokine 23-plex Assay ...
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bioRxiv - Molecular Biology 2019Quote: ... with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006, Bio-Rad). The protein was also subjected to mass spectrometry to assess its purity and molecular weight ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to α-actinin was estimated by a gel-shift assay (4%-15% gradient gel, Biorad) and determined as >95%.
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted 1:20 in 1X PBS and protein concentration was quantified by the DC protein assay with BSA as the standard (BioRad®). Lysates were normalized to 5 μg/μL in 2X loading buffer (Lysis buffer supplemented with 5 mM beta-mercaptoethanol and Coomassie blue) ...
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bioRxiv - Molecular Biology 2019Quote: ... The results were normalized to total protein content in the corresponding cell RIPA lysates (Quick Start Bradford Protein Assay, Bio-Rad). The ELISA detects both the 163S and 157S chemerin.
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bioRxiv - Molecular Biology 2020Quote: ... Glycerol was added to the supernatants (final concentration: 10%) and protein concentrations were adjusted using Bio-Rad protein assay (Bio-Rad). The protein samples were diluted in 6x loading buffer (9.8% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... using a TaqMan-based assay for lipL32 [180] in 25 μl reactions performed with SsoAdvanced Universal Probes Super Mix (Bio-Rad). Copy numbers for lipL32 were determined using an internal standard curve for the lipL32 amplicon cloned into pCR2.1 TOPO (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines in the supernatants were then measured by using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Biorad Laboratories, Hercules, CA) following the manufacturer’s standard protocol.
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bioRxiv - Microbiology 2021Quote: ... HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (Rutsaert et al. ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The amount of protein in the extracts was assessed by the Bradford method using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad). Protein electrophoresis in 10% or 12% PAA gels with SDS in 1x Laemmli buffer was carried out ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was used to quantify the total protein concentration in the sample using the DC protein assay (Bio-Rad) a BSA standard curve [34] ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of each fraction of proteins was determined by SDS-PAGE and quantitatively by Bradford protein assay (BIO-RAD, USA).
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bioRxiv - Biochemistry 2020Quote: ... Protein content was determined by BCA assay (Pierce) and proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membranes were blocked ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Immunology 2021Quote: ... the beads were re-suspended in assay buffer and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp) ...