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Citations for Bio-Rad :
3751 - 3800 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration of the supernatant was determined using the BCA protein assay (Pierce™) and 15 µg was loaded on the gel (3450124, Bio-rad), which was next turbo transferred on nitrocellulose membrane (1704159 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions pooled and the concentrations of conjugated mi3 particles were determined using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA). Conjugated nanoparticles were characterized by electron microscopy imaging and SEC as shown in Figure S1C-E ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Physiology 2022Quote: Maternal plasma and SI IEL proteins were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Mississauga, Canada) and the Luminex system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)68 ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Microbiology 2022Quote: ... The reaction rate and total protein concentration (determined by the method of Bradford with the BioRad Protein Assay reagent; BioRad, Hercules, CA) were used to determine CAT activity ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected in a 1.5 mL microtube and 10 µL of the suspension was used for live cell counting using the trypan blue exclusion assay with a TC20™ automated cell counter (Bio-Rad). BSA (0.05 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was kept and the total protein concentration was determined by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using a BSA calibration curve (MP BIomedicals #160069 ...
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bioRxiv - Microbiology 2023Quote: ... intrahepatic cccDNA values were normalized by the cell number measured by the hRPP30 copy number variation assay (Bio-Rad, Pleasanton, California, USA)[53] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by SARS-CoV-2 qRT-PCR using the Seegene Allplex™ 2019-nCoV Assay (cat. no: RP10243X) on a CFX96 Real-time PCR Detection System-IVD (Bio-Rad)
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... using gene specific primers purchased from Fluidigm (DELTAgene Assay) and detected using SsoFast™ EvaGreen Supermix with Low ROX (Bio-Rad Laboratories). Results from these assays were normalized based on the values for GAPDH mRNA.
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bioRxiv - Cell Biology 2023Quote: ... The supernatants of the cell lysates were normalized for total protein concentration according to Bradford assay (#5000006, Bio-Rad, Hercules, CA, USA), mixed with SDS loading dye ...
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bioRxiv - Biochemistry 2023Quote: ... The culture medium after incubation was analyzed for the presence of secreted human cytokines using Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, USA) and Bio-Plex 200 Systems (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... ATP contents were expressed in nmol/mg protein (using the Bio-Rad Protein Assay Cat. #500-0006, Bio-Rad, Hercules, California, USA).
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bioRxiv - Cancer Biology 2023Quote: ... RNA from the FFPE clinical samples were processed through BioRad mRNA ddPCR fusion assays for RET (ID dHsaEXD81378442, Bio-Rad, Hercules, CA), ROS1 (ID dHsaEXD73338942 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, California, USA) and concentrated to approximately 100-200 μM.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad; www.bio-rad.com) and bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were cleared by centrifugation (16000 g, 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). Proteins separated by SDS-PAGE without boiling due to the heat sensitivity of WDR62 [7] ...
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bioRxiv - Biophysics 2024Quote: ... we collected the supernatant in a new 1.5 ml reaction tube and determined the protein concentration of each sample by a Bradford protein assay (Biorad, Cat. No.: 5000006). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... The concentration was determined by a Bradford assay and the purity was estimated by SDS-polyacrylamide gel electrophoresis (PAGE) using Image Lab 6.1 software (2022, Bio-Rad Laboratories, Inc.). This expression and purification procedure was repeated for TmrA.
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes kit (Bio-Rad). Quantitation of actin transcripts was performed by a SYBR green assay ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription iScript cDNA synthesis kit and SSo Advanced Universal SYBR Green kit were from Bio-Rad (Solna, Sweden)25.
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from oocytes/embryos using PicoPure RNA isolation kit (Arcturus) and cDNA made using iScript(tm) cDNA Synthesis kit (BioRad). Brilliant III SYBR® green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from dissociated single cells using QIAGEN RNease Mini Kit and cDNA was prepared using 1µg RNA with iScript cDNA Synthesis Kit (Bio-Rad). SYBR green-based QPCR was performed using murine primers listed as below (GLI1 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated using RiboPure ™ kit and reverse transcribed using an iScript cDNA synthesis kit (170-8891; Bio-Rad). The qPCR reaction was performed using IQ SyberGreen supermix (170-8882 ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced - Universal SYBR Green Supermix ...
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bioRxiv - Biophysics 2023Quote: ... Single-stranded cDNA was synthesized from 1 µg of total RNA as template using a commercially available kit (iScript cDNA Synthesis Kit, Biorad). RT-qPCR analysis of nascent mRNA abundance was performed in duplicate using iQ SYBR Green Supermix (Biorad #1708880 ...
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bioRxiv - Molecular Biology 2023Quote: ... One aliquot was used to synthesize single-stranded cDNA starting from 1 µg of total RNA using a commercially available kit (iScript cDNA Synthesis Kit, Biorad).
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bioRxiv - Biochemistry 2020Quote: ... The B4GAT1 activity then was normalized against the amount of protein measured in the 10 µL of sample using the DC Protein Assay (Bio-Rad, Hercules, CA) with BSA as the standard ...
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bioRxiv - Physiology 2022Quote: ... and protein content assessed by BCA assay (Pierce) before 15 ug protein lysates were separated on a TGX Stain-Free™ criterion gel (Bio-Rad, Sweden). Following which ...
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bioRxiv - Microbiology 2021Quote: Levels of CFTR DNA were determined using a droplet digital PCR (ddPCR) assay on the QX100 ddPCR system (Bio-Rad Laboratories, Hercules, CA), using CFTR sense (5’-GCG ATC TGT GAG TCG AGT CTT-3’ ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were centrifuged at 16,000 x g to pellet cellular debris and protein concentration was determined by BCA quantification assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Cell Biology 2019Quote: Protein samples from all related experiments (PKC and EDC cross-linking assays and Westerns on heart lysates) were processed in lithium dodecyl sulfate sample loading buffer (Bio-Rad, 1610737 CA), heated at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... The CPG methylation of OCT 4 and NAG promoters were determined by Real-time quantitative PCR (qRT-PCR) on the GeneAmp 9600 PCR assay system (ABI Bio-Rad, Hercules, USA). PCR cycle was used as follows ...
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bioRxiv - Physiology 2021Quote: ... mRNA expression analysis was performed using an iQ SYBR Green Supermix or Taqman Gene Expression Assays with an iQ5 thermal cycler and detection system (Bio-Rad, Hercules, CA). Hypoxanthine guanine phosphoribosyl transferase (Hprt ...
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bioRxiv - Cancer Biology 2020Quote: ... the supernatant was collected and 30 µg of lysate (determined by the Bradford Assay) was diluted in 2x loading buffer (Bio-Rad, Hercules, CA) and was loaded onto 4-20% polyacrylamide gels (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were centrifuged at 12,000g for 20 mins to remove cell debris and protein concentration in supernatants was determined using DC™ Protein Assay (Bio-Rad, #5000111). Twenty μg of proteins from L ...