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Citations for Bio-Rad :
3401 - 3450 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 37-Plex kit (171AL001M; Bio-Rad) Bio-Plex Human Chemokine Panel ...
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bioRxiv - Plant Biology 2024Quote: ... iScript cDNA synthesis kit (Bio-Rad) was employed to reverse-transcribe 2 µg of the DNase I-treated RNA ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Gene expression was detected using SybrGreen RT-qPCR expression assays in a 96-wells plate format as described by the manufacturer using MyIQ real-time PCR cycler (BioRad); cDNA templates were used at a 1:40 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected for BCA assay and analyzed by SDS-PAGE using Mini-Protean® TGXTM 4– 15% (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 80μg of proteins per sample was labelled and protein estimation was done using RC-DC protein assay from Bio-Rad.
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bioRxiv - Microbiology 2019Quote: ... Protein concentrations of the DMSO control samples were measured in triplicate using a detergent compatible (DC) assay (Bio-Rad, USA). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Samples from PKC and cross-linking assays were loaded on 18% Tris-Glycine Stain-Free gels (Bio-Rad, 5678073 CA); samples from heart lysates were loaded on 10% Tris-Glycine Stain -Free gel (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... The exchanged sample was diluted to either 0.8 or 0.4mg/ml via the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, CA). Samples were divided into 100µL aliquots and flash frozen with liquid nitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant representing the nuclear fraction was collected and protein concentration of both fractions was determined using the DC protein assay (Biorad). Cytosolic and nuclear fractions were further diluted to the desired protein concentration using the corresponding lysis buffers ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Immunology 2021Quote: Multiplexed cytokine assays were performed according to the manufacturer instructions by using a Bio-Plex Pro Human cytokine screening panel (Biorad) containing 48 human cytokines ...
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bioRxiv - Biochemistry 2020Quote: ... Tissue lysates for Western blot were prepared as previously described [11] and sample protein content was determined by Bradford assay (BioRad). Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared of insoluble debris by centrifugation prior to determination of protein concentration by BCA assay (BioRad, CA, USA). Lysates were electrophoresed through either 4-12% NuPAGE or 8% E-PAGE gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of the supernatant was used to normalize the level of total protein in each sample via BCA assay (BioRad) and the supernatant was then flash frozen on dry ice ...
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bioRxiv - Neuroscience 2021Quote: ... AS and EVs were lysed in RIPA buffer and protein concentration was measured with DC Protein Assay (Biorad, 500-0116), using BSA as standard ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatants were collected and protein concentration was determined using the Bradford-based Bio-Rad protein assay (Bio-Rad). Samples were then denatured and separated using SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... Each qPCR assay was performed in 50 μl with PCR master mix (iQ™ Multiplex Powermix, Bio-Rad Life Science), 250 nM primers and 100 nM probe ...
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bioRxiv - Immunology 2020Quote: ... were determined by Luminex assays using the Luminex platform Bio-Plex Multiplex 200 (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Plasma samples from four healthy volunteer donors were used as controls.
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bioRxiv - Microbiology 2021Quote: ... SYBR green-based qPCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA). The reaction mixture (20 µL ...
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bioRxiv - Neuroscience 2019Quote: The amount of protein was prepared from homogenized tissues or tells and determined using the Bradford quantification assay (Bio-Rad). Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2020Quote: Real time quantitation of target gene to B2M mRNA was performed with a SYBR-Green real-time PCR assay using an ICycler from BioRad. The threshold cycle (Ct ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Genetics 2022Quote: ... Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs) against total protein content (calculated with the Bradford protein assay, BioRad). Each condition was measured in quadruplicates and each experiment was performed at least twice.
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration for each condition was measured directly from 1 µL aliquot of the sample using NanoDrop (record A280nm measurements and use the exact same protein sample buffer as blank) and the Bradford assay (Biorad). After incubation for different amounts of time (1 hour after dialysis or dilution to allow droplet maturation ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... 1x PIC) using a sonifier BRANSON 250.The protein in crude homogenates was determined by Bradford protein assay (BioRad, USA) and 50 μg of protein was used for a subsequent chloroform/methanol extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble extract was collected and total protein concentration determined using Protein Assay Dye Reagent Concentrate (BIO-RAD, Cat No 5000006) with BSA as standard ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was dissolved in RapiGest SF (Waters) and the protein concentration was determined (Bio-Rad protein assay, Bio-Rad). The mitoribosomal lysates were frozen in liquid nitrogen and analyzed at the Proteomics core facility of the Medical University of Vienna ...
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bioRxiv - Microbiology 2020Quote: ... 10 U of HindIII restriction enzyme and 1.1 μL of a 20X primers-probes mix (ddPCR mutant assay) provided by Bio-Rad (concentrations and sequences of probes are not provided by the manufacturer but they guaranty the validation by Minimum Information for Publication of Quantitative Real-Time PCR Experiments [MIQE] guidelines) ...
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bioRxiv - Cancer Biology 2021Quote: ... centrifuged for 20 minutes at 13,000 rpm at 4°C and the protein concentration in the supernatant was measured with an DC protein assay (BioRad). Samples were separated by SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatant was collected and analyzed by Bio-Plex ProTM Human Cytokine Th1/Th2 Assay according to the manufacturer’s instructions (Bio-Rad). All tests were performed in duplicates ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 ng of cDNA was analyzed with isoform-specific droplet digital PCR assays and ddPCR Supermix for probes (Bio-Rad). Primers and probes (Integrated DNA Technologies ...
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bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The qRT-PCR assays were carried out on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system was of 10 μl ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies used for staining plaque or TCID50 assays were goat anti-mouse horseradish peroxidase and goat anti-rabbit horseradish peroxidase (Biorad).
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bioRxiv - Immunology 2019Quote: Cytokine levels in culture medium and serum were determined by magnetic bead-based multiplex assay using Luminex technology (Bio-Rad), IL-1β ELISA (R&D Systems ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantification of protein concentration was done using the RC DC Protein Assay (Bio-Rad, Hercules, CA; Cat. No. 500–0119). Equal amounts of lysate were denatured and loaded onto a 10% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2019Quote: ... The qRT-PCR assays were carried out on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system was of 10 μl ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to myosin II was estimated by a gel-shift assay (4–15% gradient gel, Biorad) and determined as >99% for both S1 and lever-arm-less S1 (Supplementary Fig ...
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bioRxiv - Biophysics 2020Quote: ... Western blot assays were performed after resolving the samples on a 4-15 % Tris-HCl gradient gel (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were homogenized using a handheld cell disruptor and sample concentrations were determined using a DC Lowry protein assay (BioRad). For cell lysates ...
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bioRxiv - Immunology 2021Quote: ... The amount of protein in each tissue sample was quantified using detergent compatible protein assay (Bio-Rad, Hercules, CA, USA). Then ...