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Citations for Bio-Rad :
1301 - 1329 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... by tank blotting at 250mA for 90 min in a cold room (4 °C) in blotting buffer (catalog#1610734, Bio-Rad, Hercules, CA, USA). The membrane was blocked for 2 h in TBS-T (tris buffered saline supplemented with 0.1 % Tween ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at 70°C. The following primary antibodies were used (see also Suppl. Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 seconds at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 10°C during the LC-MS measurements ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Biochemistry 2023Quote: ... DsbAΔSP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 °C and then the oxidant or reductant were removed using Micro Bio-Spin™ P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP™-5 columns (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180 °C using a plate sealer (PX-1; Bio-Rad, Hercules, CA, USA) and maintained at 10 °C during the LC-MS measurements ...
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bioRxiv - Bioengineering 2021Quote: ... All assays were incubated in a thermocycler maintained at 65 °C for 60 min following which endpoint OSD fluorescence was imaged using a ChemiDoc camera (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 25 μL of double distilled water (boiled at 95 °C for 5 minutes) containing loading buffer (4X; Bio-Rad Laboratories, Mississauga, ON, Canada) and reducing agent (20X ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... The samples were heated with Laemmli sample buffer with 2-mercaptoethanol at 95°C for 20 min and separated on SDS-PAGE using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA).
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bioRxiv - Systems Biology 2022Quote: ... shortly centrifuged (2,000xg, 1 min) and the plate heated at 95°C for 60 min in a thermal cycler (Biorad’s S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories, Inc., CA, USA). ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 30 seconds and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). PCR products were cleaned using an ExoAP reaction (see Supplemental Methods ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 3:40 min and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). Amplicons were run on a 1% agarose gel and the highest molecular weight fragments (> 5kb ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein estimations were performed using the Bio-Rad microtiter Bradford protein assay (California, USA) according to the instructions provided (Bio-Rad version: Lit 33 Rev C) and measurements were made at 595 nm using a SpectraMax Plus ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated overnight at 37°C and the optical density (OD) readings at 595nm were recorded using an iMark™ Microplate Absorbance Reader (Bio-Rad laboratories, Hercules, CA, USA). Synergistic killing of planktonic cells is defined as a greater reduction in viability than can be expected when adding the effects of individual treatments 27.
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-cPLA2, 1:100, Cell Signalling; mouse anti-LaminA/C, 1:500, ABCAM; rat anti-tyrosinated α-tubulin 1:500, Bio-Rad; mouse γ-H2AX, 1:2000) in blocking solution for 1h at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... Precision Plus Protein™ Western C™ Blotting standards and Precision Protein™ Streptactin-HRP conjugate were used to estimate molecular weight (BioRad, cat# 1610380 and cat#1610376). Relative quantification was performed using ImageJ software (version 1.53p (22) ...