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Citations for Bio-Rad :
801 - 850 of 1416 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... SOB motility plates (containing 10-4 M IPTG and 2.4 g/L MgSO4) at 37°C and pictures were taken using a Gel Doc (Biorad) imager at the beginning and the end of the linear swimming motility period (from 5 to 8 hours) ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated ≈20-24 hours at 37°C and then photographed using a ChemiDoc XRS+ System (Bio-Rad). At least three independent transformants were used to perform replicates for phenotypic assays.
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bioRxiv - Biochemistry 2021Quote: ... the samples were gradually heated from 4 to 98°C with a rate of 0.2°C/10 s in the CFX96 Real-Time System C1000 Touch Thermal Cycler (Biorad). The fluorescence intensity was measured using an excitation wavelength of 470 nm while monitoring emission at 570 nm.
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Genetics 2020Quote: ... 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Molecular Biology 2022Quote: ... followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 mins at 70°C and loaded onto 4-20% polyacrylamide gradient gels (Bio-Rad) for electrophoretic separation in Tris-glycine-SDS buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were incubated for 18 to 24 h at 37°C and imaged in a ChemiDoc Imaging System (BioRad) to detect RFP and GFP fluorescence (Cy3 and Cy2 channels ...
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bioRxiv - Molecular Biology 2023Quote: ... at 95°C for 10 min and separated by electrophoresis in a 4–12% SDS–PAGE gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluent was isolated by centrifugation at 100 g for 4 min at 4°C (Bio-Rad 732-6204), buffer exchanged (Amicon Ultra 10 kDa MWCO UFC501024 ...
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bioRxiv - Physiology 2023Quote: ... and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad). Membranes were blocked in EveryBlot Blocking Buffer (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... boiled 10 min at 95°C and run in 4% - 15% Mini-Protean TGX Stain-Free gels (Bio-Rad) in non-reducing conditions (no β-mercaptoethanol nor DTT) ...
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bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
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bioRxiv - Microbiology 2023Quote: ... The resulting mixtures were put at 37°C for 30min and transferred to 0.4 cm electroporation cuvettes (Gene Pulser/MicroPulser, BioRad), which were submitted to electroporation using 10ms pulses at 180V repeated three times at one-second intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... by boiling for 10 min at 98°C before loading onto a 4-20% gel (Bio-Rad, Hercules, CA), then transferred to PVDF 0.22 m membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulse-field gel electrophoresis was carried out in a 0.9% agarose gel in 0.5× TBE at 14°C with a CHEF DR III from Bio-Rad with a constant switch time of 20 s during 24 h ...
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bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1% SDS for 10 min at 60°C and images captured using a ChemiDoc MP Imaging system (BIO-RAD).
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Cancer Biology 2024Quote: ... which were boiled at 95°C for 5 minutes before loading in pre-cast PAA gels (Bio-RAD, 4561084) and run at 100V for approx ...
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bioRxiv - Microbiology 2024Quote: ... samples were incubated at 95°C for 10LJminutes and then resolved on a TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2LJµm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 58°C for 30 sec using a CFX ConnectTM real-time PCR detection system (Bio-Rad, Philadelphia, PA, USA). List of genes validated and corresponding primer sequences are given in Table S8.
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Genetics 2021Quote: ... unspecific binding sites were blocked over night at 4°C with 3% milk powder (Marvel) in Tris Buffered Saline solution (BioRad) including 1% Tween 20 (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 14,000×g for 30 min at 4°C and equalized using quick start Bradford 1x dye reagent (BioRad, 5000205). Ni-NTA resin was washed three times with lysis buffer containing 20mM imidazole pH 8.0 and 5mM β-mercaptoethanol while samples were prepared ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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bioRxiv - Cell Biology 2022Quote: ... using the BioRad transfer system at 100 V for 60 min at 4°C or with Trans-Blot Turbo Mini PVDF Transfer Packs using Trans-Blot Turbo Transfer System (Biorad). The membrane was blocked for 30 min with the Odyssey Blocking Buffer (TBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on denaturing SDS-PAGE followed by an electrotransfer at 4 °C onto a PVDF membrane (0.2 µM, Bio-Rad). NRT2.1 was detected using three different anti-NRT2.1 antisera produced by Eurogentec against either the synthetic peptide TLEKAGEVAKDKFGK (anti-NRT2.1 19) ...
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bioRxiv - Cancer Biology 2019Quote: ... and extension at 72°C for 30 seconds using the CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.). Analysis and fold differences were determined using the comparative threshold cycle method ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...