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Citations for Bio-Rad :
1001 - 1050 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... After rinses with PBS-0.1% Tween 20 (PBST), sections were incubated overnight at 4°C with rabbit anti-Iba1 (Wako, 019-19741) and anti-BrdU (Biorad, MCA2060). After washes with PBST ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Neuroscience 2021Quote: ... and then heated to 37°C for 30 min and separated via SDS-PAGE on a Mini-Gel apparatus (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was incubated for 30 mins at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad).
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...
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bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 10% neutral buffered formalin at 4°C overnight and then mCherry-positive plaques were visualized using a ChemiDoc MP imaging system (Bio-Rad). Afterwards ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated at 37°C for 1 h with goat anti-mouse IgG StarBright Blue 520 or anti-rabbit IgG Starbright Blue 700 (Bio-Rad) secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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bioRxiv - Plant Biology 2020Quote: ... heated for 30 min at 37°C and loaded into a polyacrylamide gel (any kD™ precast protein gel, Biorad #4569034). To avoid cross-contamination ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad). Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat) ...
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bioRxiv - Biochemistry 2020Quote: ... in 1X TBE buffer at 220V for ∼13 min at 4°C and were imaged with a Gel Doc XR+ (Bio-RAD) and Quantity One 4.6.9 software (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Protein extract was then added to the mixture and gently rotated at 4°C overnight then beads were washed 4 times with CHAPS buffer and then boiled in Laemmli Sample Buffer (Bio-Rad) for 10 min ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... we used 40 cycles of denaturation at 94° C for 15 seconds followed by annealing/elongation at 60° C for 30 seconds in a C1000 thermal cycler CFX96 real-time system (Bio-Rad). We normalized gene expression to levels of β-actin mRNA ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The formaldehyde crosslinks were reversed by heating the samples for 1 h at 70 °C and RNA was extracted using PureZOL™ (BioRAD) as described in the RNA extraction section ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was cleared by centrifugation at 21000 g for 5 min at 4°C and supernatant was mixed with one-third volume 4X Laemmli Sample Buffer (Bio-Rad). For lysozyme lysis ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... according to the respective manufacturer’s protocols with an annealing temperature of 58 °C during real-time PCR in a CFX96 real-time system (Bio-Rad) thermocycler.
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bioRxiv - Synthetic Biology 2021Quote: ... microwells containing assembled protocell arrays were all incubated at 37 °C for 3 hours before imaging on a ChemiDoc MP (Bio-Rad) imaging system ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1.0 V/cm for ∼48 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The buffer was changed every ∼24 hr.
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bioRxiv - Neuroscience 2019Quote: ... Dynabead-antibody complexes were washed 3x with 1 ml IP buffer and 3x with 1X PBS and proteins were eluted at 70°C for 10 min in 2X Laemmli sample buffer (50 μl; Bio-Rad) containing 5% 2-mercaptoethanol ...
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bioRxiv - Pathology 2021Quote: ... 0.10 M) at 50°C overnight followed by cleanup on 150 μL cation exchange media (AG50WX8, Bio-Rad, Hercules, CA, US) on top of C18 SPE columns (Strata C18-E ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Cell Biology 2021Quote: ... against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad) equilibrated with the ion-exchange buffer A (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... The reaction was incubated at 37°C for 30 minutes then left to cool for 10 minutes before adding 6 μL of 0.2% 2-mercaptoethanol in SDS-page loading dye.38 Samples were then heated to 94°C for 2 minutes and added to the wells of a pre-cast 12% polyacrylamide gel (Bio-Rad). The gel was run for 45 minutes at 120 V in running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... run with radioactive sample for 15 min at 200 V, dried (80°C, 3 hours) on a gel dryer (Bio-Rad), and exposed to a phosphor screen ...
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bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...
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bioRxiv - Genetics 2021Quote: ... Samples were denatured at 100°C for 10 min and 5% of samples were loaded and run on a 10% Mini-Protean TGX gel (Bio-Rad). Electrophoresed proteins were transferred onto a nitrocellulose membrane using Turbo-blot Transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Expression and secretion samples were incubated at 95 °C for 10 minutes and then resolved on TGX Stain-free gel (Bio-Rad). Next ...
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bioRxiv - Genomics 2020Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed on a CFX96 Touch Real-Time PCR Detection System using white Hard Shell 96-well PCR plates and Microseal ‘C’ Adhesive seals (Bio-Rad) or 0.2 mL white 8-tube PCR strips and optical caps (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were incubated in the dark for 3 days at 30°C and then photographed in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... 6µg of Round or Rod-shaped CCMVTT-VLPs were mixed with 2x mercaptoethanol and heated at 95°C for 3 minutes and then loaded into Any kD Mini-PROTEAN TGX precast protein gels (BIO-RAD). Gel was run for 35min at 180V ...
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bioRxiv - Microbiology 2021Quote: ... boiled for 5 min at 95 °C and separated by discontinuous sodium dodecyl sulfate (SDS) 4-15% polyacrylamide gel electrophoresis (SDS-PAGE precast gels, Bio-Rad). The SDS-PAGE gels were stained in Coomassie Blue solution (Bio-Rad).
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bioRxiv - Genomics 2020Quote: ... FLAG immunoprecipitates were eluted at 95°C for 10 minutes and GFP immunoprecipitates were eluted at 60°C for 20 minutes into 1 X SDS-Page Buffer (Bio-Rad) supplemented with 5% BME ...
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bioRxiv - Neuroscience 2022Quote: Samples from m7GTP pull-down assays and lysates were boiled at 70°C for 10 min in Laemmli sample buffer (Bio-Rad) and resolved in 10% or 4-15% gradient SDS-PAGE gels ...