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Citations for Bio-Rad :
1251 - 1300 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (RT-qPCR) analysis was performed using a CFX384 Real-Time System (C 1000 Touch Thermal Cycler, Bio-Rad, Hercules, California) using SensiFAST SYBR No-ROS kit (Bioline ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and stored at −80□°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2023Quote: ... Primary antibody staining was performed overnight at 4&C using rabbit anti-mouse Iba-1 (1:500; Wako Chemical Cat #019-19741) and CD68 (1:200; Bio-Rad Cat #MCA1957) with secondary staining performed for 45 minutes RT using Alexa Flour 594 donkey anti-rabbit (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Cell Biology 2023Quote: ... and the lysates were heated for 15 minutes at 50 °C before being loaded onto 4-15% or 4-20% Mini-PROTEAN SDS-PAGE gels (Bio-Rad, Hercules, CA) in 1X TGS solution (250 mM Tris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 min at 70°C and then loaded on a 12% Mini-protean TGX gel (Bio-Rad Cat# 4561044). The gels were run for approximately 1 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence signal was measured at 30 s intervals at 37°C using a CFX96 touch real-time PCR system (Bio-Rad, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was performed using 3.5 μl of diluted cDNA and 5.5 μM primers listed in Table 2 (C.-M. Lee and Thomashow, 2012; Wu et al., 2008)using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, cat# 1725121) with the CFX 384 TouchTM Real-Time PCR Detection System (Bio-RAD) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: ... Equal volumes of the cell suspension (RT) and the agarose solution (75°C) were mixed and immediately transferred to plug molds (Bio-Rad Laboratories, Cat. # 1703713), ∼100 μl mixture per plug ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were transferred to C. sporogenes by conjugation as described before (Heap et al., 2009) using E. coli CA434 (E. coli HB101 (Bio-Rad Laboratories, The Netherlands) harboring the broad host IncPß+ conjugational plasmid pRK24 (Williams et al. ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Biochemistry 2022Quote: ... 10μL of each time point were mixed with RNA loading dye and heated to 95°C before loading on a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Samples were run on a 7.5% Urea PAGE gel for 30 minutes at 300V in 0.5X TBE buffer ...
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bioRxiv - Microbiology 2022Quote: ... Vesicle preparations were treated with 6xSDS loading buffer and boiled for 10 min at 100°C and run on a 10% SDS-PAGE gel (Bio-Rad Laboratories, Hercules, CA), stained for 15 min with Coomassie Brilliant Blue Stain ...
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bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Immunology 2020Quote: ... of protein were incubated in 1X Laemmli buffer with 0.1 M dithiothreitol at 65°C for 15 min and electrophoresed on 4-15% gradient TGX gels and transferred to PVDF membranes (Bio-Rad Laboratories, Mississauga, Canada). The membranes were blocked with Tris-buffered saline with 0.05% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2020Quote: ... tissue samples were incubated with 20 μl of base solution (25 mM KOH, 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Neuroscience 2023Quote: ... frozen in liquid nitrogen and stored at −80°C until the RNA isolation with Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA, USA), as described (Makarava et al. ...