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Citations for Bio-Rad :
901 - 950 of 1329 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... using pre-treated (95°C for 10 min to reduce adherence) PCR tubes and optical flat caps (Bio-rad, Belgium). The final reaction volume was 20 µL (10 µM of miniMeCP2 or 20 µM of miniMeCP2T158M) ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Microbiology 2022Quote: Real-time-PCR was performed with Maxima SYBR Green qPCR Master Mix (Applied Biosystem, #4309155) at an annealing temperature of 60°C on a CFX96 touch Real-Time PCR machine (Biorad) using the following primers:
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Genetics 2023Quote: ... DNA was resolved by electrophoresis in a 0.7% agarose gel which was dried for two hours at 50°C using 583 gel dryer model from Bio-Rad. Dried gel was hybridized in Church-Gilbert solution (1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Systems Biology 2023Quote: ... and heated at 95°C for 60 minutes in a thermal cycler (Bio-Rad S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng of purified RNA was immediately reverse transcribed at 37 °C for one hour in an S1000 Thermal Cycler (BioRad) via the OmniScript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was then eluted and reverse-crosslinked by incubating the beads at 95°C for 10 min in presence of 100 µl of 10 % Chelex resin (BioRad), treated with Proteinase K (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Microbiology 2023Quote: Protein samples were centrifuged (150,000 × g for 10 min at 4°C) and loaded onto an Enrich SEC 650 10 × 300 column (Bio-Rad) using the AKTA explore system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Microbiology 2023Quote: ... the plates were developed at 100 °C for 20 min and further analyzed with the ChemiDoc-System (Bio-Rad, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled 5min at 90°C and 10µl was loaded on a 4-20% gradient SDS-PAGE gel (Biorad #4561096). Gel was stained with Coomassie brilliant blue R.
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and the supernatant was incubated at 95°C for 10 minutes in the presence of Laemmli sample buffer (Bio-Rad). Proteins (20 μg per sample ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2024Quote: ... the samples were boiled at 95°C for 10 minutes and resolved on a TGX stain-free gel (Bio-Rad) for SDS-PAGE analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... and heat denatured at 95°C for 10 min before loading onto a 4-15% Tris-glycine gel (Bio-Rad) and electrophoresed at 125 V ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 35 °C to 79°C at a rate of 0.3 °C per 15 s in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Fluorescence intensity was determined using excitation (λem=525/20 nm ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies overnight at 4 °C and then incubated with host species-matched HRP-conjugated secondary antibodies (BioRad) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... chromosomal DNA was prepared in 1.3% of low-melting point agarose plugs as described in 74 and run at 14 °C with 1X TBE buffer in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120°angle for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... denatured for 10 min at 96°C before it was separated at a 4-20 % PROTEAN TGX stain-free gel (BioRad) at 200 V for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... Linearized plasmid was mixed with 250 μL of cells at 15°C in a 0.4 cm gap electroporation cuvette and transformed immediately by electroporation using a Gene Pulser II (Bio-Rad) set to 800V and 25 μF ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were eluted by heating at 100°C for 5 min in Laemmli sample loading buffer (Bio-Rad, USA), and resolved on an SDS/PAGE ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...