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Citations for Bio-Rad :
8951 - 9000 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10 well, 50 μl; Bio-Rad) at 160 V for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Microbiology 2021Quote: Protein samples were resolved by SDS-PAGE using 4-12% Bis-Tris NuPAGE precast gels with MES running buffer alongside the Precision Plus prestained protein standard (Bio-Rad). Proteins were transferred to nitrocellulose membranes following SDS-PAGE using an iBlot 2 Dry Blotting System (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of interest were further purified using a Superdex 200 pg column on an NGC liquid chromatography system (Bio-Rad). Each fraction was resolved on Novex 4–20% tris–glycine gels with Coomassie blue staining to assess protein purity.
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Cancer Biology 2020Quote: ... 30–40 µg of total protein were loading into each well of a 10% SDS-PAGE gel and separated using an electroporation system (Bio-Rad). Proteins were transferred onto an NC membrane (Whatman) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated via SDS-PAGE and transferred to a PVDF membrane using Trans-Blot Turbo System (Bio-rad). The following antibodies were used for detection ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Ref: 4568085) at 160V ...
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bioRxiv - Cancer Biology 2021Quote: ... Fifty micrograms of protein from each sample was loaded and separated in 7.5% SDS-PAGE gel (4561024, Bio-Rad, Hercules, CA) and transferred onto a 0.2 mm PVDF membrane using the wet transfer method (90 V for 90 min) ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of protein were then separated by SDS-PAGE on 4-20% polyacrylamide gels (Bio-Rad Laboratories, Richmond, VA, USA) at 200V for 40min ...
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bioRxiv - Neuroscience 2020Quote: ... Equivalent total protein amounts from BH and EVs were separated on 4−15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-fusion lysate (input) and proteins eluted from the beads were separated on a 4–20% TGX SDS-PAGE gel (Bio-Rad) and transferred to a PVDF membrane for immunoblot analysis ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 10 µl 4M Urea and the protein concentration of both pellet and supernatant was determined by using the method of Bradford (5000006, Bio-Rad). Finally ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg of protein were loaded per lane and electrophoresed on a 10% TGX precast SDS PAGE gel from Bio-Rad. Protein was transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... were then activated with 100% methanol and total protein extracts were transferred for 14 min at 25 V using a Trans-Blot Turbo Transfer System (Bio-Rad). Membrane-bound proteins were visualized with Ponceau S (78376 ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent amounts of protein from cellular lysates or fractions were electrophoresed on pre-casted gels (Bio-Rad Laboratories, Hercules, CA, USA) or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred into a new tube and and the protein concentration was determined using a Bradford assay (Biorad #5000006) as described by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... The labelled proteins were further purified and separated from free Alexa Fluor dye using a Superdex 75 10/300 GL gel filtration column (Cytiva) using either an ÄKTA protein purification system (Cytiva) or NGC Chromatography System (Bio-Rad). The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were transferred to a new tube and an aliquot were used for protein quantification using the Bradford assay (Bio-Rad). A total of 10-20 μg of protein lysates were separated on 8% gels by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were sonicated and then resolved by SDS-PAGE on 10% gels along with Precision Plus All Blue protein prestained standards (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes in a Turbo blotter (Trans-Blot Turbo Blotting System, 170-4155, Bio-Rad Laboratories). The total protein amount was visualized using the stain-free image of the blot (Figure 1B) ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10 µg protein was then separated by SDS/PAGE and transferred to Immuno-Blot PVDF membranes (Bio-Rad). Membranes were incubated in 5% non-fat milk for 1 hour at room temperature to block nonspecific binding ...
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bioRxiv - Genetics 2022Quote: ... The 13 µl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 2 hours 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The Cy7.5 signal corresponding to persulfidated proteins was recorded at 800nm and the NBF-adducts signal was recorded at 488nm on a Chemidoc Imager (BioRad, France). Persulfidation levels were assessed by normalizing the Cy7.5 intensity to Alexa488 (NBF-adducts ...
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bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
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bioRxiv - Bioengineering 2022Quote: ... run at 120 V constant voltage (Pharmacia LKB, ECPS 3000/150) using a Hoefer gel system and Precision Plus Protein Kaleidoscope standards (Bio-Rad). Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2022Quote: The source lysates of Brl1 APs 90 min post labeling were adjusted to 50 µL with a protein concentration of 4 µg/µL with lysis buffer as determined by the Bradford method (Bio-Rad). Samples were diluted with 200 µL guanidine chloride (7 M in 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands visualized using film exposures in the linear range were imaged using a GS-900 calibrated imaging densitometer (Bio-Rad) and quantified using Image Lab Software (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... heated for 10 min at 70C then run out on a 4-12% Novex NuPAGE gel in 1X NuPAGE MOPS buffer with a protein ladder(Bio-Rad). The gel was transferred to a 0.2um PVDF membrane for 2 hours at 200mA at 4C ...
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bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Genomics 2021Quote: ... Proteins were subjected to electrophoresis through a 12% Glycine SDS-PAGE gel (Mini-PROTEAN TGX Stain-Free Precast gel, Bio-Rad) and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of protein were then resolved by SDS-PAGE on either 7.5% or 10% polyacrylamide gels (Bio-Rad, Hercules, CA). Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... and eletro-transfer at 100V for 2h were performed to separate protein extracts and transfer them to PVDF membranes (Bio-Rad). Non-specific sites were blocked with 5% BSA in TBS 0.05% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 20 μg of protein was separated using a 3–8% Criterion™ XT tris-acetate or a 4–15% Criterion™ XT tris-glycine protein gels and then transferred to PVDF membrane with a Trans-Blot Turbo Transfert system (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Fifty microliters of homogenate were used to measure cytokine and chemokine protein abundance using a Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples with normalized protein content were loaded into each lane (20 μg/lane) and resolved by SDS-PAGE (Bio-Rad, 456809). Total protein contents were imaged with the Bio-Rad Chemidoc MP Imaging System ...
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bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked and probed overnight with corresponding primary antibodies (MERTK ...