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Citations for Bio-Rad :
9001 - 9050 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... resolved on an Anykd™ Criterion™ TGX™ Precast Midi Protein SDS-PAGE gel (cat. no. 5671124, Bio-Rad) and electrotransferred onto Immun-Blot PVDF membranes (150-160 μg/cm2 ...
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bioRxiv - Immunology 2024Quote: ... 75 μg of protein per sample was mixed with 1× Laemmli sample buffer (cat. no. 1610747, BioRad, Hercules, CA, USA), resolved on an Anykd™ Criterion™ TGX™ Precast Midi Protein SDS-PAGE gel (cat ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... the protein bands on the SDS-PAGE gels were scanned and quantified using a ChemiDoc MP imaging system (Bio-Rad) and Image J software ...
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bioRxiv - Molecular Biology 2024Quote: ... the separated proteins were transferred to a 0.2 μm PVDF membrane using the Trans-Blot Turbo transfer system (Bio-Rad) according to the mixed molecular weight protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μg of total protein from each sample was prepared by mixing with 6.25 μL of Laemmli Buffer 4X (Bio-Rad), 1.25 μL β-mercaptoethanol (BME) ...
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bioRxiv - Microbiology 2024Quote: ... Immunoblotting was performed as described above in “Protein photocrosslinking and analysis” using anti-SNARE antibodies and Tidyblot (1:1000; BioRad) in 5% nonfat milk in TBST ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were diluted to a concentration of 75 μg protein in 25 μL native sample buffer (Bio-Rad, catalog #1610738) and lysis buffer to a final volume of 50 μL ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant proteins corresponding to the SUMO-IQ fusion of myosin XI-A were purified using Profinity Ni-IMAC column (BioRad) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration of the isolated EVs samples was determined using the Bradford assay with a reactive solution from BioRad, following the instructions provided by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... the protein signals in the membrane were visualized and recorded using SuperSignal West Pico PLUS Chemiluminescent Substrate by Bio-Rad chemiluminescence system ...
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bioRxiv - Microbiology 2024Quote: ... boiled for 5 min at 95°C and the proteins were separated on 10–20% gradient polyacrylamide gels (Criterion precast gel, Biorad). The virus proteins were visualised by staining with Coomassie Brilliant Blue.
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was conducted by loading 2μg protein/lane on a 4% to 15% TGX stain-free gel (Bio-Rad) nonreducing and 100 mM DTT reducing conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Neuroscience 2024Quote: ... Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well (ThemoFisher, NP0321BOX) and transferred to polyvinylidene fluoride (PVDF) membranes (Biorad, 1704156EDU). PVDF membranes were blocked with 5% nonfat dried milk in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... protein bands (IP or TCE) were transferred from polyacrylamide gel to a PVDF membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 8% blocking milk in Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was transferred to a clean tube and protein concentration was determined using a Bradford assay (Bio-Rad). 200 μg tissue homogenate or 10 μg cell lysate was diluted in 50 μl CB lysis buffer and was used for the reaction with CatB Substrate (RR-amino-4-trifluoromethyl coumarin (AFC)).
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bioRxiv - Neuroscience 2024Quote: ... 80-100 μg of protein were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Gel (Biorad, 4561093EDU, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were denatured in Laemmli loading buffer as previously described,30 25 µg of protein was separated by 7.5% SDS-polyacrylamide acrylamide gel electrophoresis (SDS-PAGE) (Criterion, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... and the separated proteins were transferred onto PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked using 3% BSA in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Biochemistry 2024Quote: ... lysates were then spun down at 14,000 rpm for 10 mn before determining protein concentration using Bradford reagent (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by gel electrophoresis and transferred to nitrocellulose membranes using a semi-dry blotting system (Bio-Rad Laboratories). Membranes were blocked with 3% milk or 5 % BSA in TBST for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Cholesterol values were calculated using known cholesterol solutions and normalized to protein content as measured by DC assay (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: Polar flagellins purified from bacterial growths of each mutant strain (2 µg) were separated by 12% SDS-PAGE as described previously [31] and stained with Coomassie Blue total protein stain (BioRad), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...
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bioRxiv - Cell Biology 2024Quote: ... Equivalent micrograms of protein from each sample were diluted using 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA, Cat # 1610747) supplemented with β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were detected with Super ECL Detection Reagent (Yeasen, 36208ES60) in a ChemiDoc™ MP Imaging System (Bio-Rad). Native proteins were extracted using Column Tissue & Cell Protein Extraction Kit (Epizyme ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD and pA-LS NP protein expression was analyzed using SDS-PAGE and subsequent Western blot on PVDF membrane (Biorad) using StrepMAB-Classic HRP 1:3000 (2-1509-001 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... 7.5 µg of proteins were separated on polyacrylamide gel and then transferred on nitrocellulose membrane by the Trans-Blot dry system (Biorad). Membranes were blocked on milk (5% ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissue lysates containing equal amounts of total protein were mixed with 4x Laemmli Sample Buffer (Bio-Rad, Hercules, CA, USA) and incubated at 96 °C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of proteins at 10 µM mixed in a 3:1 ratio with 4X Laemmli Buffer Sample (Biorad) provided with 10% DTT ...
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bioRxiv - Plant Biology 2024Quote: ... the indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-PROTEAN TGX precast gel; Bio-Rad) and blotted either on PVDF Immobilon-P (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... and 10-20 μg of total protein were separated by SDS-PAGE using a 4-20% gradient (Bio-Rad 4561094), transferred to polyvinylidene difluoride membranes (EMD Millipore IPFL00010 ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of total protein per sample was separated using SDS-PAGE on 10% stain-free polyacrylamide gels (Bio-Rad) and then transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting tissue lysate was centrifuged at 13,800 g for 15 minutes and the protein concentration was determined using a Bradford assay (Bio-Rad). The protein extracts were then separated by SDS-PAGE on 12% gels and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... For preparation of western bot: eluted proteins were separated by SDS-PAGE and subsequent transfer to nitrocellulose membrane (Bio-Rad). Membranes were then blocked in tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were loaded and resolved by polyacrylamide gel electrophoresis (PAGE) using Criterion TGX 4–20% Precast Gels (Biorad) and transferred onto PVDF membrane using iBlot gel transfer system (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The results were normalised to the protein concentration of the homogenates obtained in a Bradford assay (5000006, Bio-Rad, UK).
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bioRxiv - Molecular Biology 2024Quote: ... Four micrograms of protein/sample were loaded into 4%– 20% Mini-PROTEAN TGX stain-free polyacrylamide gels (Bio-Rad; 4568093), transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Cellular lysate was prepared from each samples and an equal amount of protein was loaded into a single lane of the 4-15% SDS-polyacrylamide gel (BioRad). After electrophoresis and transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto a nitrocellulose membrane using Trans-Blot Turbo Transfer Pack (BIO-RAD, Cat. No. 1704158). Ponceau S was used to check the completeness of the protein transfer ...
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bioRxiv - Microbiology 2024Quote: ... the proteins are transferred from the gel to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad), using the pre-programmed turbo transfer program ...
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bioRxiv - Molecular Biology 2024Quote: The SDS-PAGE was used to run the prestained protein markers before they were transferred onto PVDF or nitrocellulose membranes (BioRad) at 80 mA for one hour using the transfer buffer Tris-glycine SDS (48 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... and VMAT2-associated proteins were separated from the beads using SDS Tris–Glycine sample buffer (Bio-Rad, Hercules, CA, USA) and heating (10min at 70°C) ...
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bioRxiv - Physiology 2024Quote: ... 20 μl of the protein sample was loaded on 4-20% Criterion TGX Gels (Bio-Rad Laboratories, Hercules, CA, USA). Gels were transferred onto 0.2 μm nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... on 4-20 % Mini-PROTEAN TGXTM Stain-Free Protein Gels (Bio- Rad) and 1x Tris/Glycine/SDS buffer (Bio-Rad). Proteins were then transferred onto a Trans- Blot TurboTM Mini PVDF Transfer membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 170 V for one hour in 1X Tris/glycine/SDS running buffer ...