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Citations for Bio-Rad :
9051 - 9100 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 4-HNE-conjugated protein bands between 100-25 kDa were visualized by the chemiluminescence imaging system (Chemi-Doc, Bio-Rad). Membranes were stained with Ponceau S dye to verify equal loading and transfer of proteins.
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bioRxiv - Physiology 2024Quote: ... The separated S-nitrosylated proteins were then analyzed using 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules). A monoclonal anti-Cx43 antibody (#C8093 ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein samples were resolved on a 7.5% SDS-PAGE gel (7.5% Mini-PROTEAN TGX Precast Protein Gels, 10-well; Bio-Rad, #4561023). The proteins were transferred onto a PVDF membrane and blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Neuroscience 2024Quote: ... were electrophoresed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and transferred proteins from gel onto polyvinylidene difluoride fluoride (PVDF) membranes (BioRad). After transformation ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were separated by SDS-PAGE on a 10% acrylamide gel using the Mini- PROTEAN Tetra System (Bio-Rad). Migration was performed in 1X Tris-Glycine electrophoresis buffer for 30 minutes at 100 V to gather the proteins ...
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bioRxiv - Biophysics 2024Quote: ... Protein band intensities were measured via densitometric analysis of Western blot data using the Image Lab software from Bio-Rad.
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bioRxiv - Biophysics 2024Quote: ... The protein samples were analyzed for yield and purity by SDS-PAGE Criterion gels in a Dodeca cell (Bio-Rad). Proteins with visible expression by SDS-PAGE were analyzed for homogeneity by analytical size exclusion chromatography on a BioInert HPLC (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred on nitrocellulose membrane at 100V for 2 h using the Mini trans-blot cell (Bio-Rad, #1703930). Membranes were blocked with 5% skim milk diluted in PBST (PBS + 0.1% Tween 20 ...
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bioRxiv - Genomics 2024Quote: GATA4 DBD purity was evaluated with SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4× loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Biochemistry 2024Quote: Protein stability was measured in triplicates by differential scanning fluorometry49 using a CFX Opus 96 Real-Time PCR System (BioRad) in a 96-well reaction plate with 20 μl sample ...
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bioRxiv - Biophysics 2024Quote: ... A total of 15 µg of protein was loaded into a 4-20% TGX 10-wells gel (Bio-Rad, USA) and the transfer step was performed using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... Protein samples were separated by SDS-PAGE using Mini-PROTEAN TGX Stain-Free Precast 4-15% Gels (Bio-rad; 4568083) and transferred onto a PVDF membrane (Bio-rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a methanol-activated PVDF membrane (Cytiva) using a Mini Trans-Blot Transfer Cell (Bio-Rad) at a constant 200 mA and 4 °C for 2.5 hours in cold transfer buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein were loaded in 2x Lämmli Buffer containing β-Mercaptoethanol on a Tris-Glycin-Gel (4568096, BioRad). Immunoblots were transferred on membranes (Trans-Blot Turbo Transfer System ...
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bioRxiv - Cell Biology 2024Quote: For detection of protein band by WB protein bands were transferred from polyacrylamide gel to polyvinylidene fluoride (PVDF) membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 10% blocking milk (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were boiled for 10 min at 95°C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747).
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were generated by sonicating and boiling 1*10^6 cells in 100ul of 2X Laemmli Buffer (Bio-Rad) with BME ...
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bioRxiv - Biochemistry 2024Quote: Protein purity was analysed using SDS-PAGE 4-15% or 4-12% gels with a molecular weight ladder (Precision Plus Protein, Biorad) as a control ...
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bioRxiv - Bioengineering 2024Quote: Protein concentration levels of lysates were analyzed using a BCA protein assay and quantified using a colorimetric 96-well microplate reader (Biorad). A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescently labeled protein was diluted to 10 nM according to Amax and aliquoted by mixing 10 µl of protein + 10 µl of 2x Laemmli sample buffer (Biorad). Therefore ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto PVDF membranes (Amersham Hybond PVDF, #10600023 – 0.45 µM (300mm)) using a wet transfer system (Bio-Rad) at constant 80V for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Supersignal West Femto (Pierce) incubation was used to visualize the protein bands of interest on the ChemiDoc XRS+ Imaging system (Biorad).
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bioRxiv - Cancer Biology 2024Quote: ... A 13-point standard curve was generated to extrapolate the soluble protein levels and the data was analyzed using the Bioplex Manager Software (Biorad).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins (10-15 μg) were resolved on 4-15% SDS-PAGE gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... The separated proteins were then transferred to Trans-Blot Turbo Midi 0.2 μm PVDF membranes (1704157, Bio-rad, the U.S.A) using the Trans-Blot Turbo System (2,5 A ...
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bioRxiv - Cancer Biology 2024Quote: Protein extracts were subjected to SDS-PAGE and transferred to a nitrocellulose membrane by wet western blot transfer (Bio-Rad). The membrane was blocked in TBST buffer (TBS + 1% Tween ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins on the gel were transferred to a Trans-Blot Turbo Midi 0.2 µm PVDF Transfer Packs (Bio-Rad) nitrocellulose membrane using the Mix MW program of the Trans-Blot Turbo Transfer System ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by SDS–PAGE under denaturing conditions using 4- 20% Mini-PROTEAN® TGXTM precasted polyacrylamide gels (Biorad). Proteins were transferred to nitrocellulose membranes (Biorad) ...
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bioRxiv - Genetics 2024Quote: ... The rabbit polyclonal anti-Sakura antibodies were affinity purified using His-MBP-Sakura recombinant protein and Affigel-l5 (Bio-rad), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein quantifications were determined with a Bradford assay then 30μg of protein was separated on a 8-16% or 12% pre-cast gel (Bio-Rad) using primary antibodies diluted 1:500-1:1000 and secondary antibodies (1:2500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Upon removal of genomic DNA (TURBO DNA-free™ Kit or RNase-free DNase Set) cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). PCR reactions were run in triplicate with 6.25-12.5 ng cDNA per reaction using the CFX96 Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using the reverse transcription kit (PrimeScript RT reagent Kit) and quantitative (q)PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The housekeeping gene GAPDH was used for reference ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the cytosolic fraction from each sample was measured using the Bradford assay (Protein Assay Dye Reagent Concentrate; Bio-Rad). After normalization ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Aliquots of larval homogenate were removed prior heat-treatment to measure soluble protein using a Bradford assay (Bio-Rad 500-0006) with BSA standard curves.
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat. # 500-0205) from Biorad. This was followed by colorimetric estimation using TECAN Infinite M200 pro-multimode plate reader in 96-well format ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated using an Any kD gel (SDS-PAGE) and transferred to a nitrocellulose membrane (BioRad, Temse, Belgium). Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad ...
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP was normalized to total protein bands per lane (visualized by a stain-free scan on a Bio-rad gel doc). Each experiment was repeated at least two times from biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...