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Citations for Bio-Rad :
8751 - 8800 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography for MEZ was run on a S200 HiLoad 16/600 column at 0.5mL/min with protein standards from Bio-Rad to determine the oligomeric state of MEZ in solution ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Immunology 2020Quote: ... Equal volumes of 30 μg total protein were denatured by adding Sample Loading Buffers (BIO-RAD Laboratories; Hercules, CA, USA). Denatured protein samples were loaded onto 4-20% Tris-Glycine Gel Novex WedgeWell (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 12.5-50 μg of protein was mixed with one third volume of 4X Laemelli Sample Buffer (Bio-Rad Laboratories 1610747) and boiled at 100 °C before loading into a polyacrylamide gel alongside either the PageRuler™ or PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE and then transferred on PVDF membranes using the Trans-Blot Turbo blotting system (Biorad). Pyocins S5 and AP41 were detected using rabbit specific polyclonal affinity-purified ɑ-S5 and ɑ-AP41 antibodies ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Microbiology 2020Quote: Supernatants of DMSO-and RAP-treated 1B4int schizonts samples following egress and invasion in Albumax-free RPMI 1640 medium were subjected to SDS-PAGE on 16.5% Mini-PROTEIN® Tris-Tricine gels (BioRad). Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade ...
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bioRxiv - Biochemistry 2020Quote: Aliquots (250 μg total protein) of hippocampal extracts of WAR animals subjected to freeze/thaw were treated with 5 μL of dithiothreitol (Biorad) 50 μg/μL ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were homogenized using a handheld cell disruptor and sample concentrations were determined using a DC Lowry protein assay (BioRad). For cell lysates ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins migrated for 60 minutes at 200 V and were then transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories) for 2 hours (70 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were loaded on 4-12% Bis-Tris gels and run in 1X MOPS SDS Running Buffer in the Mini Gel Tank according to manufacturer’s Precision Plus Protein Dual Color Standards (Bio-Rad). Following electrophoresis ...
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bioRxiv - Synthetic Biology 2020Quote: ... Soluble and insoluble fractions were separated by centrifugation at 20,000 x g for 30 minutes and protein expression was screened by running both fractions on SDS-PAGE (Bio-Rad) and for selected samples also by negative stain EM ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred onto PVDF membrane by wet western transfer using the Mini Trans-Blot® Cell Assembly (Bio-Rad) in buffer containing 25 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad), according to the supplier’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Samples (1 µg total protein) were loaded onto non-reducing 12% Mini-PROTEAN® TGX™ precast gels (Bio-Rad), and molecular weight shifts and relative band intensity used to detect the presence and relative abundance of the crosslinked complex ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were detected by scanning the gel for green (GFP) or red (mCherry) fluorescence using a ChemiDoc MP system (BioRad) and proteolytic cleavage was assessed from the changes in banding patterns ...
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bioRxiv - Genetics 2020Quote: ... TGX stain-free gels had total protein visualized prior to transfer and analyzed on Image Lab software (Image Lab 6.0, Bio-Rad) 41 ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: Tissue lysates (30 μg protein) were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against ROCK1 (Cat# ...
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bioRxiv - Neuroscience 2021Quote: ... 20 to 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 was use to block the membranes ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total protein/sample were loaded in each well of a 10% PAGE gel and transferred onto a nitrocellulose membrane (#1704158, BioRad) in a Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... ECM and RPE cell lysate (15-20 μg protein) were run separately on precast Mini-Protean TGX gels (Bio-Rad) and transferred onto PVDF membrane (Immobilon) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were separated in 10% SDS-polyacrylamide gels and electrotransferred to PVDF membranes using a semi-dry system (Bio-Rad). Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a nitrocellulose membrane using a Mini Trans-Blot Cell Wet/Tank blotting system (BioRad). After transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
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bioRxiv - Biochemistry 2021Quote: ... Ni-NTA and FLAG elutions were analyzed by SDS-PAGE on AnykD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). The samples were not heated before loading to preserve the fluorescence of the GFP and mCherry tags ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized via chemiluminescence (ECL Enhanced Chemiluminescence; Pierce ECL Western Blotting Substrate) using the ChemiDoc MP system (BioRad).
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bioRxiv - Developmental Biology 2021Quote: ... Loading was normalized to total protein for monoubiquityl-Histone H2A and Ubiquitin blots using AnyKD TGC Stain-Free gels (BioRad). Loading for monoubiquityl-Histone H2B blots was normalized to mouse anti-β-tubulin (1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... The amount of ATP for one cell was derived from the amount of protein in one cell found via Bradford assay (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were separated on 10 or 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... and equal volume and quantity of protein samples were made by addition of 4x Laemmli Sample Buffer (#1610747, Bio-Rad) containing 2-merchatoethanol (#1610710 ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Supersignal West Femto (Pierce) incubation was used to visualize the protein bands of interest on the ChemiDoc XRS+ Imaging system (Biorad). Images acquisition and densitometry analysis was performed using the Image Lab software (Biorad).
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bioRxiv - Cancer Biology 2020Quote: ... Around 40 μg of whole-cell lysate was resolved in 10% SDS-PAGE gel (Mini protein Tetra cell Bio-Rad) and blotted to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... and the separated proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The immunodetected protein bands were then analyzed using ChemiDoc XRS system with Quantity One software (Bio-Rad, Richmond, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... and 30 μg of total protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). Correct protein loading was confirmed by Ponceau staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled with SDS sample buffer and resolved on a 10% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
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bioRxiv - Microbiology 2021Quote: hCMP-mRBD protein kinetic binding studies to ACE2-hFc and CR3022 antibody were performed on a ProteOn XPR36 Protein Interaction Array V.3.1 (Bio-Rad). The GLM sensor chip was activated with sulfo-NHS and EDC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and vasopressin) peptides were spotted onto a 0.2-μm polyvinylidene fluoride membrane (Immun-Blot PVDF Membrane for Protein Blotting, BIO-RAD). The membrane was air dried at room temperature and was washed for 10 min in 0.05 M Tris buffer (pH 7.6 ...