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Citations for Bio-Rad :
8851 - 8900 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to a nitrocellulose membrane (Cytiva) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad). The membrane was blocked in 5% milk in TBS-T (tris-buffered saline ...
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bioRxiv - Immunology 2023Quote: ... Buffer containing eluted protein was filtered through a 0.22-μm spin filter and directly loaded on the Enrich SEC650 column (Bio-Rad) for SEC ...
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bioRxiv - Neuroscience 2022Quote: ... were resolved by SDS-PAGE using stain-free precast gels (4-15% Mini-Protean Tgx Stain-Free Protein Gels, Biorad). Gels were then activated by Ultraviolet light with the ChemiDocTM Touch Imaging System (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and equal amounts of proteins were subjected to SDS-PAGE (TGX Stin Free FastCast Acrylamid, 12%, Bio-Rad, 161-0185) and transferred onto nitrocellulose membrane (Transblot Turbo ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The luminescence was calculated as an average of three technical replicates and normalized to the total protein levels quantified using the Bradford’s method (Bio-Rad). Results are from the two or three biological replicas and are expressed as a percentage of the normalized luminescence of the control.
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bioRxiv - Cancer Biology 2022Quote: ... Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad) staining.
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... A representative size-exclusion chromatography chromatogram is shown in Figure S4 with a gel filtration protein standard (Bio-Rad Laboratories). The fractions from the single monomer peak were combined and diluted ten-fold and buffer exchanged three times into 10 mM sodium phosphate buffer at pH 6.5 with an Amicon Ultra-15 Centrifugal Filter Unit Ultracel-10 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents of ~1.5×106 cells per lane were separated by 10% SDS-PAGE along with the Precision Plus Protein Kaleidoscope standard (BioRad). The fractionated proteins were transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2024Quote: ... 20μg of total protein per fraction were collected and diluted with their respective solvents and 4X Laemmli buffer (Biorad, 1610747) to obtain aliquots of equal volume ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a hybond ECL nitrocellulose membrane using a wet transfer system (Bio-Rad Laboratories, Hercules, CA, USA). The membrane was incubated in α-Ty antibody (1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... and purity was determined using SDS-PAGE on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) stained with Biosafe Coomassie (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated in SDS-PAGE gels were transferred onto a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The protein concentrations in each cell extract were measured with the Bradford assay (SmartSpec Plus spectrophotometer, Bio-Rad, Hercules, CA) using the Bradford dye reagent and bovine standards (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... The adapted protein quantity per lane was loaded into 4-20% 1.5 mm Tris-glycine 15 well mini gels (Bio-Rad) with 1X Laemmli sample buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 μg protein per sample were loaded onto SDS-page gels for protein separation and then transferred onto nitrocellulose or methanol-activated PVDF membranes via full-wet transfer assay (BioRad) or semi-wet transfer ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 180 V for one hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 30µg of total proteins were loaded onto each well of Mini-PROTEAN TGX Stain-Free Precast Gels (10%, Biorad, # 4568031), and migrated for 45 min at 120 Volts ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by electrophoresis through 12% agarose polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad Cat #1620175) by electroblotting ...
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bioRxiv - Immunology 2024Quote: ... protein was transferred to a 0.45-μM nitrocellulose membrane (Cytiva) with a Transblot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked with 5% BSA and 0.1% Tween 20 (IB blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Developmental Biology 2023Quote: ... and sample aliquots were diluted 1:50 in water before Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were normalized to 1 mg/mL in 0.1% Azo ...
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Genetics 2023Quote: ... The supernatant was transferred to a fresh 1.5 ml Eppendorf tube and protein concentration was measured using Bradford assay (Bio-Rad Protein assay Dye reagent Concentrate (#5000006 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins on the gel were transferred to a polyvinylidene fluoride membrane using a Transblot SD semi-dry transfer system (BioRad) at 25V for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... wet blotting (at 200 mA per gel for 1 hour) was performed to transfer proteins onto 0.2 µm nitrocellulose membranes (Bio-Rad). Total protein staining was performed according to the manufacturer’s protocol (Revert™ Total Protein Stains kit ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad) ...