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Citations for Bio-Rad :
9101 - 9150 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were electrophoresed for 70 min at 80 V in a BioRad MINI-PROTEAN Tetra system (BioRad Laboratories, CA, USA), and the gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein (typically 10 μg) were separated under reducing conditions on 10 or 12 % TGX-polyacrylamide gels (Bio-Rad) and transferred to Hybond-P membrane (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Cell Biology 2022Quote: ... were normalized by the level of total proteins in each lysate (determined using the TGX-stain free imaging technology; Bio-rad).
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Biochemistry 2020Quote: ... was confirmed by both SDS-PAGE using a precast Mini-PROTEIN® TGX Stain-Free™ Gel (Bio-Rad Laboratories, Inc.) and by measuring the direct absorbance at 280 nm using a NanoDrop™ Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The amount of bound protein was determined by boiling in SDS sample buffer and analysing using Bradford reagent (BioRad, Hercules, CA). The eluted protein was resolved on a BioRad 15% TGX gel ...
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bioRxiv - Immunology 2021Quote: ... Lysates were collected and equal protein amounts were separated by SDS-PAGE and blotted onto a nitrocellulose membrane by wet-blotting (Bio-Rad). Membranes were blocked with TBS-T containing 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... of total protein for each sample were separated by SDS-PAGE (10% acrylamide gel) and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked in TBST buffer containing 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and the proteins in the gel were transferred to a PBDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). After probing the membrane with the primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12%) and transblotted onto nitrocellulose membranes (Bio-rad, California, USA). The membranes were blocked with 5% dry milk (overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heat denatured and equal concentrations of protein were electrophoresed on 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad) and transferred onto 0.2 µm PVDF membrane in Tris-Glycine transfer buffer containing 20 % methanol ...
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bioRxiv - Cell Biology 2020Quote: ... Band intensities were quantified in ImageJ FIJI and normalized to total protein content quantified with the Stain-Free technology (Bio-Rad). Stain-Free total protein signals are shown as loading controls in the figures.
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bioRxiv - Biochemistry 2020Quote: ... 40 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline with+ 0.1% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... of ssDNA-Cy5-SWCNT and FAM-protein at 2X working concentration were added via multichannel pipette into a 96-well PCR plate (Bio-Rad) and mixed by pipetting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 8μg of proteins per well were loaded onto precast 12% Criterion™ XT Bis-Tris polyacrylamide 26 wells gels (Bio-Rad) and electrophoresis was achieved by applying a tension of 200V during 60 min using a Criterion™ Cell with the NuPAGE MOPS SDS running buffer (1X) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Two protein standards were run in the first two lanes of each gel: BioRad Kaleidoscope Pre-stained Standard (BioRad, #161-0324) and Magic Mark XP Western Standard (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 10% of the bead volume was transferred to a new tube and were boiled in 30 μL of 2X protein loading buffer (Biorad, 1610737) supplemented with 2 mM biotin and 20 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2021Quote: ... The cAMP levels were normalized to the total protein concentration in the supernatant determined by Bradford Assay using BSA standard curve (Bio-Rad). The final cAMP concentration is expressed as pmol cAMP per mg of total protein ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to westernblot analysis using precast polyacrylamide gels (10-20% Tris-HCl or 4-12% Bis-Tris, Bio-Rad). For immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Incubation with appropriate HRP-conjugated secondary antibodies (Thermo) was for 2 hours at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD). Densitometric analysis was performed using ImageJ software (NIH).
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Criterion TGX Stain-Free Precast Gels (Bio-Rad) and transferred onto Immobilon-P membranes (MilliporeSigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM β-mercaptoethanol) until the washes indicate a protein concentration of less than 0.1 mg/mL by Bradford assay (Bio-rad). The protein was eluted with elution buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The denatured samples were loaded onto AnyKD™ Mini-PROTEAN® TGX™ precast protein gels (Bio-Rad, Hercules, CA, USA) and separated for 40 min at 40 mA ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... The proteins were characterized by electrophoresis using a 12% SDS polyacrylamide gel and imaged using a ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Zoology 2021Quote: ... These standardized data were then normalized to total protein from the same membranes using a SYPRO Ruby Blot Stain (Bio-Rad). The averages of phosphorylated MAPK/total MAPK/total protein in the hearts from treatment fish ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
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bioRxiv - Genetics 2021Quote: ... The 13 μl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 90 min ...
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bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10 well, 50 μl; Bio-Rad) at 160 V for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Microbiology 2021Quote: Protein samples were resolved by SDS-PAGE using 4-12% Bis-Tris NuPAGE precast gels with MES running buffer alongside the Precision Plus prestained protein standard (Bio-Rad). Proteins were transferred to nitrocellulose membranes following SDS-PAGE using an iBlot 2 Dry Blotting System (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of interest were further purified using a Superdex 200 pg column on an NGC liquid chromatography system (Bio-Rad). Each fraction was resolved on Novex 4–20% tris–glycine gels with Coomassie blue staining to assess protein purity.
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Cancer Biology 2020Quote: ... 30–40 µg of total protein were loading into each well of a 10% SDS-PAGE gel and separated using an electroporation system (Bio-Rad). Proteins were transferred onto an NC membrane (Whatman) ...