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Citations for Bio-Rad :
9101 - 9150 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Collected protein samples were boiled with 1× Laemmli buffer before SDS-PAGE procedure with Mini-PROTEAN TGX Precast Gel (Bio-Rad) in Tris/Glycine Buffer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was loaded (10-50 µg per well, depending on protein of interest) and transferred using semi-dry turbo blotter (Bio-Rad). Blots were placed into 5% blocking solution containing primary antibodies and were incubated overnight at 4°C (Table 2) ...
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bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates containing approximately 40 μg of protein were boiled for 10 min prior to separation by 4-20% SDS-PAGE (BIO-RAD). After transferring at 90V to a nitrocellulose membrane for 90 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of proteins were separated on either 10% SDS/PAGE gels or 4-20% Mini-PROTEAN TGX Stain-Free Gels (#4568094, Bio-rad). Proteins were then transferred onto PVDF membranes and probed with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... The total content of glycogen was normalized to content of soluble protein measured using the Bradford assay (Bio-Rad 500–0006) with BSA standard curves.
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bioRxiv - Molecular Biology 2022Quote: ... A calibration curve was generated by measuring the elution volumes of a series of standard proteins of known molecular mass (Bio-Rad). The molecular masses of pAgos complexes were calculated by interpolating their elution volume onto the calibration curve ...
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bioRxiv - Immunology 2022Quote: ... protein concentration was evaluated using a Bradford protein assay (adjusted to 1µg / µl in 1% sodium dodecyl sulphate) and additionally corrected by the evaluation of protein band intensities using a stain-free acrylamide solution (TGX FastCast, Bio-Rad). Prior to blotting bands were visualized by an imaging system (ChemiDoc ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo Nitrocellulose Transfer Pack and Transfer System (Bio-Rad). The membrane was blocked for 1 h with 5% skim milk in PBS supplemented with 0.05% Tween 20 (PBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (BioRad, California, USA), which were blocked with 5% skim milk for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Biochemistry 2022Quote: ... Equal quantities of protein were mixed with Laemmli sample buffer and loaded into a gradient SDS-PAGE gel (Ref 4561086 Bio-Rad). The proteins were transferred from the gel onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Membranes were visualized by chemiluminescence and under epi-blue to visualize total protein No-Stain labeling using a ChemiDoc MP imaging system (Bio-Rad). Densitometric analysis was performed using ImageLab software (Bio-Rad).
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... the displayed S-trimer protein bands on SDS-PAGE gels were scanned and quantified by ChemiDoc MP imaging system (Bio-Rad) and ImageJ.
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Twenty-five µg of protein from each sample was ran (100 V, 100 min) on polyacrylamide gels (Cat. No. 5671095, Bio-Rad) using tris-glycine SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (25 µg) were separated by electrophoresis on 4-15% mini-PROTEAN TGX precast gels (Bio-Rad, cat# 456-1083) and transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification of the relative protein expression in ΔPr-A9 cells compared to the WT cells was determined in Image Lab software (Bio-Rad). The average signal of duplicate spots subtracting background value from negative control spots represented the levels of each protein ...
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bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
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bioRxiv - Physiology 2022Quote: ... diluted with 5X Laemmli buffer and boiled for 5 minutes at 95°C before being subjected to SDS-polyacrylamide gel electrophoresis using 15% (made in-house) or 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Physiology 2022Quote: ... after which the separated protein was transferred to a polyvinylidene fluoride (PVDF) membrane in a Trans-Blot Turbo System (Bio-Rad) at 25 V (1.3 A ...
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bioRxiv - Physiology 2022Quote: 20 µg of protein were loaded and resolved on 10% TGX fast cast gels (cat#1610173, Bio-Rad, Hercules, California, USA) for 30 min at 250 V ...
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 60 μg of each protein sample was loaded on the polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membrane (TransBlot Turbo, Bio-Rad) with semi-dry transfer (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Western blot analysis was performed by loading 7.5 g of total protein per lane into 7.5% Tris-glycine SDS-PAGE gels (Bio-Rad, catalog #4561026) and transferring to methanol-activated PVDF membranes (Bio-rad ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
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bioRxiv - Bioengineering 2022Quote: ... SDS page was performed on the samples and the proteins were transferred to nitrocellulose membranes using wet transfer (Bio-Rad Laboratories). The membranes were blocked with PBS with 0.1% Tween-20 (PBST ...
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bioRxiv - Cell Biology 2022Quote: ... The same amount of proteins for each lysate was then separated by SDS-PAGE on 4-15 % precast gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Cell Biology 2024Quote: The proteins were separated on polyacrylamide gels and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were blocked with 5% NFDM in TBST (50 mM Tris/HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins transferred from SDS-polyacrylamide gels to nitrocellulose membranes were visualized using a ChemiDoc analysis system (Bio-Rad, Hercules, CA, USA). Several exposures were analyzed to determine the linear range of the chemiluminescence signals ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then loaded onto the gel and run on ice for 45 min at 150 V using 10 µL Precision Plus Protein Standards All Blue (Bio-Rad) as ladder ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...