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Citations for Bio-Rad :
8901 - 8950 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Biophysics 2021Quote: ... We centrifuged the dialysis contents to remove any insoluble protein and then purified using cation exchange chromatography (BioRad UNOsphere Rapid S column) with an NaCl gradient ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGCTM chromatography system with EnrichTM SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Microbiology 2022Quote: ... Brain homogenates were assayed at neat for all analytes and results were normalised to their total protein concentrations (Bio-Rad, Hercules, California).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was detected using an ECL system (Amersham Pharmacia, Piscataway, NJ, USA) and was analysed using Quantity One software (BIO-RAD, USA).
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Biophysics 2019Quote: ... The concentration of the dialyzed protein was estimated using the Quick Start®Bradford Dye Reagent 1X (BIO-RAD Cat. #500-0205). The protein was stored at 4 °C before use.
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad, Catalog number: 4561083) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2019Quote: ... and 50 μg protein per sample were run on an SDS-PAGE gel and transferred to an Immun-Blot PVDF Membrane (Bio-Rad Laboratories) using standard protocols ...
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bioRxiv - Physiology 2019Quote: ... Diluted proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient Criterion TGX Precast Midi Protein gel (Bio-Rad, CA), and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Biochemistry 2020Quote: ... protein was eluted from the beads by boiling for 5 minutes at 95°C in Laemmli Sample Buffer (BioRad, catalog #161-0747). Proteins were stored at −80°C until western blotting.
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bioRxiv - Bioengineering 2021Quote: ... Produced protein was sequentially purified by affinity chromatography using Ni-NTA agarose beads and fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography system, Biorad, Hercules, CA). Protein fractions detected at λ = 280 were collected ...
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bioRxiv - Bioengineering 2020Quote: ... Tumors were homogenized and lysed using RIPA buffer and the total protein concentrations were determined using DC assay reagent (Bio-Rad Laboratories). Cell lysates (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... A gel slice corresponding to 100 to 150 kd was isolated (based on size markers in an adjacent lane; Precision Plus Protein™ Dual Color Standards from Bio-Rad). The gel slice was transferred to a D-Tube Dialyzer Midi Tube (EMD-Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The protein extract was first separated by loading equal amounts of protein on 4-20 % Mini-PROTEAN® TGX™ Gel SDS–PAGE gradient gels (456-1096, Biorad) and then transferred to the nitrocellulose membrane with Trans-Blot Turbo Transfer Pack (170-4159 ...
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bioRxiv - Physiology 2021Quote: ... 10 μg of total protein for each sample was separated by SDS-PAGE and transferred to Immun-Blot PVDF membrane (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant protein extracts were subjected to SDS-PAGE and proteins were transferred to a nitrocellulose membrane according to manufacturer’s instructions (Bio-RAD, Hercules, CA). Protein blots were first probed with GL2-specific antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 3–8% Tris-acetate gels (Life Technologies. Proteins were transferred to nitrocellulose membrane by semi-dry transfer (Trans-Blot Turbo transfer system; Bio-Rad) or ...
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bioRxiv - Molecular Biology 2020Quote: ... LC3B-I and LC3B-II protein levels were measured by densitometric analysis of Western blots and normalized to the amount of total protein as determined by densitometric analysis of stain-free gels using ImageLab™ software (Bio-Rad). Using the normalized values as determined previously by densitometric analysis ...
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bioRxiv - Cell Biology 2021Quote: ... 20μg of protein were loaded into each lane of a Criterion™ XT Bis-Tris Precast Gel 4-12% (Bio-Rad, UK) for SDS-Page separation ...
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Neuroscience 2021Quote: A 30 μL aliquot of either medium or lysate for each sample was heated at 95 °C for 10 min and loaded on two separate SDS-PAGE gels (Any kD™ Mini-PROTEAN® TGX Stain-Free Protein Gels; BioRad). Proteins were electrophoretically transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25V ...
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bioRxiv - Synthetic Biology 2021Quote: ... The treated samples were subjected to SDS-polyacrylamide gel electrophoresis on 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The separated protein samples were then transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 25 μg of protein was loaded onto a 4–20% Mini-PROTEAN® TGX™ Precast Gel (Bio-Rad) and transferred onto a PVDF membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were mixed with standard Laemmli loading buffer and 30 μg protein loaded on mini-Protean TGX precast gel (Bio-Rad, USA). After SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated under reducing and non-reducing conditions on Mini-Protean TGX Any-kD precast gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose membranes (GE / Amersham ...