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Citations for Bio-Rad :
8951 - 9000 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... a part of each sample was run on a 4-15% Criterion TGX Stain-Free Protein Gel (26 wells, Bio-Rad, #5678085) in 1x SDS running buffer at 200 V for 32 min ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 4°C for 15 minutes at 16,000 g and protein concentration was measured by the Bradford method (Bio-Rad, Hercules, USA). Next ...
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bioRxiv - Molecular Biology 2022Quote: 15 µg of nuclear extract in SDS loading buffer or 30 µg of total protein extract were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto 0.22 μm nitrocellulose membranes with the semi-dry transfer protocol in TurboBlot®-buffer (Bio-Rad Laboratories, Inc.). The membrane was blocked with Tris-buffered saline ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected by chemiluminescence using the Clarity™ Western ECL Substrate coupled to a ChemiDoc XRS+(Bio-Rad, Hercules, CA, USA). Quantification of Western blots relied on ImageJ software [42].
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... except that the protein was further purified by anion exchange chromatography with an Econo-pac High Q cartridge (BIO-RAD, Hercules, CA) before it was concentrated.
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bioRxiv - Neuroscience 2022Quote: ... the TCE was activated by UV light (300 nm) and the cross-linked proteins were imaged with the ChemiDoc MP Imaging System (Bio-Rad Laboratories) as a loading control (Ladner et al ...
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bioRxiv - Cell Biology 2022Quote: ... Appropriate volumes of samples were diluted to provide equal protein amounts and used for SDS-PAGE (BIO-RAD, Mini-PROTEAN TGX Gels). Western blot analysis was performed on 3 samples for wild-type and banprw337 mutants ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE of the re-oxidized samples was performed on a 4-15% Criterion TGX precast midi protein gel (Bio-Rad, 5671085) under non-reducing conditions ...
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bioRxiv - Cell Biology 2022Quote: ... equal amounts of lysate were loaded into wells of 12% Mini-PROTEAN® TGX 4-20% pre-cast protein gel (BIO-RAD) installed in Mini-PROTEAN® tetra vertical electrophoresis cell filled with 1X TG-SDS solution (Euromedex) ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 μg of total protein was loaded onto 8 to 15 % SDS-PAGE mini gels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Microbiology 2021Quote: ... Equal total protein was loaded from each of the indicated cell lysates and run on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad). Protein was transferred at 4°C to nitrocellulose membrane for 2 hours at 50V ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad, USA). The details of protein purification protocols like TCA/Acetone ...
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Plant Biology 2021Quote: ... Samples of about 120-160 μg were loaded into wells of a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad Laboratories) and run for 2.5 h at 90 V in buffer consisting of 25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then boiled at 95°C for 10 mins and resolved in 4-15 % Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) for immunoblot analysis ...
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bioRxiv - Plant Biology 2021Quote: ... Afterwards protein bands were transferred to a nitrocellulose membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad, Hercules, CA). After incubation with 5% nonfat milk in TBST (50mM Tris-Cl ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract was loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Biorad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... 40μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Membrane was blocked with 5% BSA for 60 min at room temperature on shaker ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg/lane of S was run on 4–20% Mini- PROTEAN® TGX Stain-Free™ Protein Gels (BioRad, Cat#4568081), transferred to nitrocellulose (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal protein (20ug) content were combined with loading buffer and separated using standard gel electrophoresis on 4-20% polyacryllamide gel (Bio-Rad Techonolgies) and then partial-wet transferred to nitrocellulose membranes (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... 40 mg of total protein was loaded onto a 12-15% polyacrylamide gradient gel (Mini-PROTEAN TGX Precast Mini Gel; Bio-Rad Laboratories) and transferred to a nitrocellulose membrane following separation ...
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were sectioned by freezing microtome at 50 μm intervals and immunohistochemical staining was performed for protein gene product 9.5 (PGP 9.5; rabbit polyclonal; AbD Serotec, a Bio-Rad Company, Kidlington, UK) using a free-floating protocol ...
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bioRxiv - Pathology 2019Quote: ... Affi-gel 10 affinity resins were covalently cross-linked to his-tagged GT198 protein as antigen for affinity purification of anti-GT198 according to the manufacturer’s protocol (Bio-Rad, Hercules, CA). FFPE tumor sections or tumor microarrays were deparaffinized and dehydrated through xylene and ethanol series ...
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bioRxiv - Biophysics 2019Quote: ... Fractions were analyzed after separation in stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad). These stain-free gels contain trihalo compounds that undergo UV-induced covalent modification of tryptophan residues for subsequent fluorescent detection ...
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bioRxiv - Biophysics 2019Quote: ... The purified protein samples obtained after the final SEC step were analyzed by stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad).
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bioRxiv - Biophysics 2019Quote: ... was utilized to monitor the elution of the proteins and fractions were analyzed after separation on 4– 15% precast gradient TGX Stain-FreeTM gels (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Physiology 2019Quote: ... The immunoreactive protein bands were revealed by ECL prime Western blotting detection reagent (Ammersham™) and detected using ChemiDoc™ Imaging System (Biorad). Densitometry quantification was performed using image lab software (v5.2.1 ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1X phosphatase inhibitor cocktail and equal amounts of protein samples were loaded and run in a 4 % to 20 % SDS gel (Bio-Rad Laboratories). Protein was transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Lysates were collected and equal protein amounts were separated by SDS-PAGE and blotted onto a nitrocellulose membrane by wet-blotting (Bio-Rad, Germany). Membranes were blocked with 5% milk or 5% BSA in TBS containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Microbiology 2019Quote: The purity of the proteins was checked using SDS-PAGE electrophoresis in a vertical electrophoretic mini-cell unit (Bio-Rad, Hercules, CA), in Tris-glycine running buffer (25mM Tris ...
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bioRxiv - Plant Biology 2019Quote: ... The mixed beads were washed once with the washing buffer and biotinylated proteins were eluted by 30 μL 2× SDS sample buffer and separated by SDS-PAGE (Biorad, Hercules, CA).
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bioRxiv - Microbiology 2020Quote: ... 20 μL of normalized lysates were loaded into pre-cast 4-20% SDS-PAGE gels (4-20% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad) alongside size standards (Precision Protein Plus™ ...
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bioRxiv - Developmental Biology 2020Quote: ... and separated protein was transferred from gels to polyvinylidene fluoride membranes (PVDF) using a Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). The membrane was blocked with 5% Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Total 20 to 25 μg of protein samples were resolved by sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE; Bio-Rad, Madrid, Spain) followed by Western blot ...
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bioRxiv - Pathology 2021Quote: ... CST #2118) in 5% bovine serum albumin (total protein antibodies) or 5% dry milk (phospho-specific antibodies) Tris buffered saline (Bio-Rad #1706435) with 0.1% Tween-20 ...
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bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein (20 µg) was separated electrophoretically on SDS polyacrylamide gels and then transferred to a nitrocellulose membrane (BIO-RAD Laboratories, Mississauga, ON). After blocking ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) and transferred to a nitrocellulose membrane (Bio-Rad, Canada). The expression levels of ABCB1 ...
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bioRxiv - Microbiology 2020Quote: ... Forty μg of total proteins were separated by electrophoresis on 10% polyacrylamide gels and transferred to PVDF membranes (Bio-Rad, CA, USA). The membranes were blocked with 5% BSA in Tris-buffered saline for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were run under reducing conditions on an Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gel (BioRad) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Neuroscience 2019Quote: Protein extracts were loaded at 20μg per lane and resolved by standard electrophoresis in 4-20% Mini-PROTEAN TGX Precast polyacrylamide protein gels (Bio-Rad, Hercules, CA). Gels were transferred onto polyvinyl difluoride membranes (PVDF ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... using 7.5 % polyacrylamide gels for Rad53 detection or a gradient of 4-15 % polyacrylamide for all other proteins in Mini-PROTEAN® electrophoresis chambers (Bio-Rad). Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Bioengineering 2020Quote: Mouse tissues from mdx4cv mice treated with or without AAV9-NG or AAV9-iNG were lysed with cold RIPA buffer supplemented with protease inhibitors and extracted protein samples were separated by SDS-PAGE (BioRad, 4-15%) and transferred onto Nitrocelluloase membranes (0.45 μm) ...